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Specifications for L.S. Starrett C12-12 1/2x12ft Chrome T 681-65418:
miltenyi 美天旎磁珠CD25 MicroBeads II, human – lyophilized
CD25 MicroBeads II were developed for positive selection or depletion of CD25+ cells.
miltenyi 美天旎磁珠CD25 MicroBeads II, human – lyophilized
Background information
CD25, the low-affinity interleukin-2 receptor alpha chain (IL-2Rα), is expressed on activated T and B cells and monocytes. CD25 is also present on a subset of non-activated CD4+ T cells, which has regulatory function.
The CD25 MicroBeads II recognize epitope A of the CD25 molecule.
For control staining of cells separated with CD25 MicroBeads II, it is recommended to use MACS® CD25 Antibodies, which bind to epitope B of the CD25 antigen.
For isolation of CD25+ cell subsets, the CD25 MicroBeads II can be combined with MACS® Cell Isolation Kits or MultiSort Kits. The CD4+CD25+ Regulatory T Cell Isolation Kit is recommended for most efficient separation of CD4+CD25+ regulatory T cells.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.
EPTOMICS代理Atg12 (ATG12) antibody Eptomics公司抗体供应商Recommended ApplicationsWB, IHC, ICC, IP, FC
详细介绍
EPTOMICS代理Atg12 (ATG12) antibody Eptomics公司抗体供应商
Rabbit Monoclonal Antibody
Description
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy (1). It is essential for Atg12 conjugation, and the ATG12-ATG5 conjugate is necessary to form autophagic vesicles (2). The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required (3).
Western blot analysis on (A) THP-1, (B) HepG2, (C) Raji, and (D) fetal kidney lysates using anti-Atg12 RabMAb (cat. #3473-1).
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 RabMAb (cat. #3473-1).
Western blot analysis on (A) Atg12 transfected 293T and (B) 293T lysates using anti-Atg12 RabMAb (cat. #3473-1).
Specificity
A synthetic peptide corresponding to residues in human Atg12 was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
ATG12 antibody, APG12 antibody, APG12L antibody, FBR93 antibody, HAPG12 antibody, Ubiquitin-like protein ATG12 antibody, Autophagy-related protein 12 antibody
Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8. 2. Phillips AR et al. Genetics. 2008 Mar;178(3):1339-53. 3. Williams RA et al. Autophagy. 2009 Feb;5(2):159-72.