标签归档：染料

ProLite 橙色蛋白凝胶染料* 5000X * 货号18000-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ProLite 橙色蛋白凝胶染料* 5000X *

ProLite 橙色蛋白凝胶染料* 5000X *

ProLite 橙色蛋白凝胶染料* 5000X * 货号18000 货号 18000 存储条件 在零下15度以下保存, 避免光照
规格 100 ul 价格 1164
Ex (nm) 484 Em (nm) 586
分子量 486.72 溶剂 Water
产品详细介绍

简要概述

Prolite Orange是一种蛋白凝胶染料，可用于替代SYPRO Orange蛋白凝胶染料（SYPRO是ThermoFisher的商标）。 Prolite Orange是一种灵敏的即用型荧光染料，可在一维凝胶中检测总蛋白。 Prolite Orange的灵敏度比传统的银染技术更好。 可以使用标准的UV或蓝光透射仪或包含适当滤镜或激光的成像设备查看染色的蛋白质。 荧光染色快速且高度灵敏，可检测蛋白质电泳凝胶和膜中的总蛋白质。 

点击查看光谱

产品说明书

样品分析方案

储存

存放在-20°C避光的地方。 按建议存放时，产品自收到之日起至少稳定12个月。 可用醋酸或缓冲液稀释的ProLite Orange可在4°C的玻璃或塑料瓶中保存三个月，避光。 打开之前，应先将每个小瓶加热至室温，然后在微量离心机中短暂离心，以将DMSO溶液沉积在小瓶底部。 如果存在染料颗粒，请短暂超声处理试管或剧烈涡旋试管。

 

工作溶液配制

ProLite Orange工作溶液（5000X）
用7.5％（v / v）乙酸稀释5000X ProLite Orange储备溶液，制成1X ProLite Orange储备溶液，并剧烈混合。
注意：工作溶液最多可以重复使用四次。 但是，我们观察到第二次重用后响应开始明显减少。 强烈建议使用全新的工作溶液以获得最佳效果。

 

操作步骤

建议使用以下方案，并且可以将其用作准则。但是，可能需要进行一些比较才能确定哪一种可以更好地满足您的需求。

 

电泳后染色蛋白

1.运行凝胶。
2.将工作溶液倒入一个小的塑料皿中。
注意：对于一到两个标准尺寸的小凝胶，请使用约50 mL的工作溶液。对于较大的凝胶，请使用500至700 mL的工作溶液。
注意：确保添加足够的工作量以完全浸没凝胶。
3.将凝胶放入工作溶液中。
注意：用铝箔盖住容器，以防止染料受光。
4.在室温下轻轻搅拌凝胶10至60分钟。
5.用7.5％的乙酸短暂冲洗。
6.可以在标准的300 nm紫外线透射仪或蓝光透射仪上观察凝胶。
7.脱色：大部分凝胶可通过在0.1％Tween®20中孵育过夜来脱色。或者，在7.5％乙酸的多种变化中孵育最终将去除所有污渍。

 

参考文献

Fluorescent thermal shift-based method for detection of NF-κB binding to double-stranded DNA.
Authors: Leitner, Peter D and Vietor, Ilja and Huber, Lukas A and Valovka, Taras
Journal: Scientific reports (2021): 2331

Ligand binding to a humanized anti-cocaine mAb measured by dye absorption spectroscopy.
Authors: Kirley, Terence L and Norman, Andrew B
Journal: Biochemical and biophysical research communications (2021): 93-98

Thermal Shift Assay for Exploring Interactions Between Fatty Acid-Binding Protein and Inhibitors.
Authors: Hao, Jiaqing
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 395-409

Thermal shift assay to probe melting of thrombin, fibrinogen, fibrin monomer, and fibrin: Gly-Pro-Arg-Pro induces a fibrin monomer-like state in fibrinogen.
Authors: Crossen, J and Diamond, S L
Journal: Biochimica et biophysica acta. General subjects (2021): 129805

A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics.
Authors: Kirley, Terence L and Norman, Andrew B and Wetzel, Hanna N
Journal: Journal of immunological methods (2020): 112676

Intrinsic Differential Scanning Fluorimetry for Fast and Easy Identification of Adeno-Associated Virus Serotypes.
Authors: Rieser, Ruth and Penaud-Budloo, Magalie and Bouzelha, Mohammed and Rossi, Axel and Menzen, Tim and Biel, Martin and Büning, Hildegard and Ayuso, Eduard and Winter, Gerhard and Michalakis, Stylianos
Journal: Journal of pharmaceutical sciences (2020): 854-862

SYPRO Orange – a new gold standard amyloid probe.
Authors: Mora, Aruna K and Nath, Sukhendu
Journal: Journal of materials chemistry. B (2020): 7894-7898

nanoDSF: In vitro Label-Free Method to Monitor Picornavirus Uncoating and Test Compounds Affecting Particle Stability.
Authors: Real-Hohn, Antonio and Groznica, Martin and Löffler, Nadine and Blaas, Dieter and Kowalski, Heinrich
Journal: Frontiers in microbiology (2020): 1442

Destructive twisting of neutral metalloproteases: the catalysis mechanism of the Dispase autolysis-inducing protein from Streptomyces mobaraensis DSM 40487.
Authors: Fiebig, David and Storka, Juliana and Roeder, Markus and Meyners, Christian and Schmelz, Stefan and Blankenfeldt, Wulf and Scrima, Andrea and Kolmar, Harald and Fuchsbauer, Hans-Lothar
Journal: The FEBS journal (2018): 4246-4264

Evaluation of fluorescent dyes to measure protein aggregation within mammalian cell culture supernatants.
Authors: Oshinbolu, Sheun and Shah, Rachana and Finka, Gary and Molloy, Mike and Uden, Mark and Bracewell, Daniel G
Journal: Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) (2018): 909-917

ProLite 橙色蛋白凝胶染料* 5000X * 货号18001-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ProLite 橙色蛋白凝胶染料* 5000X *

ProLite 橙色蛋白凝胶染料* 5000X *

ProLite 橙色蛋白凝胶染料* 5000X * 货号18001 货号 18001 存储条件 在零下15度以下保存, 避免光照
规格 1 ml 价格 2388
Ex (nm) 484 Em (nm) 586
分子量 486.72 溶剂 Water
产品详细介绍

简要概述

Prolite Orange是一种替代性蛋白质染料，可用于替代SYPRO Orange蛋白凝胶染料（SYPRO是ThermoFisher的商标）。 Prolite Orange是一种灵敏的即用型荧光染料，可在一维凝胶中检测总蛋白。 Prolite Orange的灵敏度比传统的银染技术更好。 可以使用标准的UV或蓝光透射照明器或包含适当滤镜或激光的成像设备查看染色的蛋白质。 荧光染色快速且高度灵敏，可检测蛋白质电泳凝胶和膜中的总蛋白质。 

点击查看光谱

产品说明书

样品分析方案

储存

存放在-20°C避光的地方。 按建议存放时，产品自收到之日起至少稳定12个月。 可用醋酸或缓冲液稀释的ProLite Orange可在4°C的玻璃或塑料瓶中保存三个月，避光。 打开之前，应先将每个小瓶加热至室温，然后在微量离心机中短暂离心，以将DMSO溶液沉积在小瓶底部。 如果存在染料颗粒，请短暂超声处理试管或剧烈涡旋试管。

 

工作溶液配制

ProLite Orange工作溶液（5000X）
用7.5％（v / v）乙酸稀释5000X ProLite Orange储备溶液，制成1X ProLite Orange储备溶液，并剧烈混合。
注意：工作溶液最多可以重复使用四次。 但是，我们观察到第二次重用后响应开始明显减少。 强烈建议使用全新的工作溶液以获得最佳效果。

 

操作步骤

建议使用以下方案，并且可以将其用作准则。但是，可能需要进行一些比较才能确定哪一种可以更好地满足您的需求。

 

电泳后染色蛋白

1.运行凝胶。
2.将工作溶液倒入一个小的塑料皿中。
注意：对于一到两个标准尺寸的小凝胶，请使用约50 mL的工作溶液。对于较大的凝胶，请使用500至700 mL的工作溶液。
注意：确保添加足够的工作量以完全浸没凝胶。
3.将凝胶放入工作溶液中。
注意：用铝箔盖住容器，以防止染料受光。
4.在室温下轻轻搅拌凝胶10至60分钟。
5.用7.5％的乙酸短暂冲洗。
6.可以在标准的300 nm紫外线透射仪或蓝光透射仪上观察凝胶。
7.脱色：大部分凝胶可通过在0.1％Tween®20中孵育过夜来脱色。或者，在7.5％乙酸的多种变化中孵育最终将去除所有污渍。

 

参考文献

Fluorescent thermal shift-based method for detection of NF-κB binding to double-stranded DNA.
Authors: Leitner, Peter D and Vietor, Ilja and Huber, Lukas A and Valovka, Taras
Journal: Scientific reports (2021): 2331

Ligand binding to a humanized anti-cocaine mAb measured by dye absorption spectroscopy.
Authors: Kirley, Terence L and Norman, Andrew B
Journal: Biochemical and biophysical research communications (2021): 93-98

Thermal Shift Assay for Exploring Interactions Between Fatty Acid-Binding Protein and Inhibitors.
Authors: Hao, Jiaqing
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 395-409

Thermal shift assay to probe melting of thrombin, fibrinogen, fibrin monomer, and fibrin: Gly-Pro-Arg-Pro induces a fibrin monomer-like state in fibrinogen.
Authors: Crossen, J and Diamond, S L
Journal: Biochimica et biophysica acta. General subjects (2021): 129805

A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics.
Authors: Kirley, Terence L and Norman, Andrew B and Wetzel, Hanna N
Journal: Journal of immunological methods (2020): 112676

Intrinsic Differential Scanning Fluorimetry for Fast and Easy Identification of Adeno-Associated Virus Serotypes.
Authors: Rieser, Ruth and Penaud-Budloo, Magalie and Bouzelha, Mohammed and Rossi, Axel and Menzen, Tim and Biel, Martin and Büning, Hildegard and Ayuso, Eduard and Winter, Gerhard and Michalakis, Stylianos
Journal: Journal of pharmaceutical sciences (2020): 854-862

SYPRO Orange – a new gold standard amyloid probe.
Authors: Mora, Aruna K and Nath, Sukhendu
Journal: Journal of materials chemistry. B (2020): 7894-7898

nanoDSF: In vitro Label-Free Method to Monitor Picornavirus Uncoating and Test Compounds Affecting Particle Stability.
Authors: Real-Hohn, Antonio and Groznica, Martin and Löffler, Nadine and Blaas, Dieter and Kowalski, Heinrich
Journal: Frontiers in microbiology (2020): 1442

Destructive twisting of neutral metalloproteases: the catalysis mechanism of the Dispase autolysis-inducing protein from Streptomyces mobaraensis DSM 40487.
Authors: Fiebig, David and Storka, Juliana and Roeder, Markus and Meyners, Christian and Schmelz, Stefan and Blankenfeldt, Wulf and Scrima, Andrea and Kolmar, Harald and Fuchsbauer, Hans-Lothar
Journal: The FEBS journal (2018): 4246-4264

Evaluation of fluorescent dyes to measure protein aggregation within mammalian cell culture supernatants.
Authors: Oshinbolu, Sheun and Shah, Rachana and Finka, Gary and Molloy, Mike and Uden, Mark and Bracewell, Daniel G
Journal: Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) (2018): 909-917

Q4ever Green核酸染料 *2000X DMSO 溶液* 货号17608-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Q4ever Green核酸染料 *2000X DMSO 溶液*

Q4ever Green核酸染料 *2000X DMSO 溶液*

Q4ever Green核酸染料 *2000X DMSO 溶液* 货号17608 货号 17608 存储条件 在零下15度以下保存, 避免光照
规格 100 ul 价格 1776
Ex (nm) 503 Em (nm) 527
分子量 溶剂 Water
产品详细介绍

简要概述

实时聚合酶链反应（real-time PCR），也称为定量聚合酶链反应（qPCR），是一种基于聚合酶链反应（PCR）的流行的分子生物学实验室技术。它可以在PCR期间（即实时）检测目标DNA分子的扩增情况，而不是像常规PCR那样在结束时进行检测。实时PCR可以定量（定量实时PCR）和半定量（即高于或低于一定数量的DNA分子）（半定量实时PCR）使用。实时PCR中有两种检测PCR产物的常用方法，包括（1）结合任何双链DNA的非特异性荧光染料； （2）由寡核苷酸组成的序列特异性DNA探针，所述寡核苷酸用荧光报告物标记，仅在探针与其互补序列杂交后才允许检测。对于第一种方法，对DNA结合染料进行PCR的实时检测有两个要求，即（a）与双链DNA结合时增强荧光；（b）对PCR的抑制作用最小。 SYBR Green主要用于各种qPCR应用中。我们最近开发了新一代的SYBR Green Q4ever Green，用于解决SYBR Green的一些局限性，例如酶抑制。 Q4ever Green可以在PCR中使用，几乎没有PCR抑制作用，并提高了灵敏度。 Q4ever Green可用于检测任何双链DNA序列的扩增。假设您的PCR引物设计合理且反应特性良好，则无需探针，可以减少测定设置和运行成本。作为SYBR Green，主要缺点是它可能产生假阳性信号。即因为Q4ever Green染料与任何双链DNA结合。它也可以与非特异性双链DNA序列结合。设计良好的引物不扩增非靶序列，并进行熔解曲线分析，这一点极为重要。

产品说明书

实验方案

储存在-20°C，避光。 按建议存放时，产品自收到之日起至少稳定12个月。

 

工作溶液配制

Q4ever Green工作溶液（50X）
使用水或TE缓冲液稀释2000X Q4ever Green储备溶液以制成50X Q4ever Green储备溶液。

 

操作步骤

建议使用以下协议。 如果需要，可以调整方案以达到最佳效果。

设置PCR反应如下：
5 µL 10X聚合酶缓冲液（不含镁）
2.5 µL的50 mM MgCl2
2 µL 50X Q4ever Green工作溶液
2 µL的5 mM dUTP
1-5个单位的DNA聚合酶
加入所需的cDNA
每个引物100-1000 nM（正向和反向引物的终浓度）
用dH2O将最终体积调整为50 µL。
在热循环荧光计上执行实时PCR并记录荧光信号。

 

参考文献

Direct detection of Helicobacter pylori from biopsies of patients in Lagos, Nigeria using real-time PCR-a pilot study.
Authors: Ajayi, A and Jolaiya, T and Smith, S I
Journal: BMC research notes (2021): 90

MinION Nanopore-based detection of Clavibacter nebraskensis, the corn Goss’s wilt pathogen, and bacteriomic profiling of necrotic lesions of naturally-infected leaf samples.
Authors: Xu, Renlin and Adam, Lorne and Chapados, Julie and Soliman, Atta and Daayf, Fouad and Tambong, James T
Journal: PloS one (2021): e0245333

A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor.
Authors: Shi, Bing and Li, Yuanming and Wu, Di and Wu, Wenming
Journal: The Analyst (2020): 2767-2773

A quantitative loop-mediated isothermal amplification assay for detecting a novel goose astrovirus.
Authors: He, Dalin and Yang, Jing and Jiang, Xiaoning and Lin, Yun and Chen, Hao and Tang, Yi and Diao, Youxiang
Journal: Poultry science (2020): 6586-6592

A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase.
Authors: Purhonen, Janne and Banerjee, Rishi and McDonald, Allison E and Fellman, Vineta and Kallijärvi, Jukka
Journal: Nucleic acids research (2020): e87

Amplification Curve Analysis: Data-Driven Multiplexing Using Real-Time Digital PCR.
Authors: Moniri, Ahmad and Miglietta, Luca and Malpartida-Cardenas, Kenny and Pennisi, Ivana and Cacho-Soblechero, Miguel and Moser, Nicolas and Holmes, Alison and Georgiou, Pantelis and Rodriguez-Manzano, Jesus
Journal: Analytical chemistry (2020): 13134-13143

Comprehensive Data of P53 R282 Gene Mutation with Human Papillomaviruses (HPV)-Associated Oral Squamous Cell Carcinoma (OSCC).
Authors: Ekalaksananan, Tipaya and Wongjampa, Weerayut and Phusingha, Pensiri and Chuerduangphui, Jureeporn and Vatanasapt, Patravoot and Promthet, Supannee and Patarapadungkit, Natcha and Pientong, Chamsai
Journal: Pathology oncology research : POR (2020): 1191-1199

Detection of Phytophthora infestans by Loop-Mediated Isothermal Amplification, Real-Time LAMP, and Droplet Digital PCR.
Authors: Ristaino, Jean B and Saville, Amanda C and Paul, Rajesh and Cooper, Donald C and Wei, Qingshan
Journal: Plant disease (2020): 708-716

Detection of extended-spectrum beta-lactamase cefotaxime resistance and virulence genes in Escherichia coli by duplex quantitative real-time PCR and melt curve analysis.
Authors: Aijuka, M and Buys, E M
Journal: Letters in applied microbiology (2020): 54-60

Development of an EvaGreen based real-time RT-PCR assay for rapid detection, quantitation and diagnosis of goose calicivirus.
Authors: Lin, Su and Zhang, Shizhong and Wang, Shao and Xie, Kaichun and Jiang, Dandan and Xiao, Shifeng and Chen, Xiuqin and Chen, Shaoying
Journal: Molecular and cellular probes (2020): 101489

Q4ever Green核酸染料 *2000X DMSO 溶液* 货号17609-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Q4ever Green核酸染料 *2000X DMSO 溶液*

Q4ever Green核酸染料 *2000X DMSO 溶液*

Q4ever Green核酸染料 *2000X DMSO 溶液* 货号17609 货号 17609 存储条件 在零下15度以下保存, 避免光照
规格 2 ml 价格 23868
Ex (nm) 503 Em (nm) 527
分子量 溶剂 Water
产品详细介绍

简要概述

实时聚合酶链反应（real-time PCR），也称为定量聚合酶链反应（qPCR），是一种基于聚合酶链反应（PCR）的流行的分子生物学实验室技术。它可以在PCR期间（即实时）检测目标DNA分子的扩增情况，而不是像常规PCR那样在结束时进行检测。实时PCR可以定量（定量实时PCR）和半定量（即高于或低于一定数量的DNA分子）（半定量实时PCR）使用。实时PCR中有两种检测PCR产物的常用方法，包括（1）结合任何双链DNA的非特异性荧光染料； （2）由寡核苷酸组成的序列特异性DNA探针，所述寡核苷酸用荧光报告物标记，仅在探针与其互补序列杂交后才允许检测。对于第一种方法，对DNA结合染料进行PCR的实时检测有两个要求，即（a）与双链DNA结合时增强荧光；（b）对PCR的抑制作用最小。 SYBR Green主要用于各种qPCR应用中。我们最近开发了新一代的SYBR Green Q4ever Green，用于解决SYBR Green的一些局限性，例如酶抑制。 Q4ever Green可以在PCR中使用，几乎没有PCR抑制作用，并提高了灵敏度。 Q4ever Green可用于检测任何双链DNA序列的扩增。假设您的PCR引物设计合理且反应特性良好，则无需探针，可以减少测定设置和运行成本。作为SYBR Green，主要缺点是它可能产生假阳性信号。即因为Q4ever Green染料与任何双链DNA结合。它也可以与非特异性双链DNA序列结合。设计良好的引物不扩增非靶序列，并进行熔解曲线分析，这一点极为重要。

产品说明书

实验方案

储存在-20°C，避光。 按建议存放时，产品自收到之日起至少稳定12个月。

 

工作溶液配制

Q4ever Green工作溶液（50X）
使用水或TE缓冲液稀释2000X Q4ever Green储备溶液以制成50X Q4ever Green储备溶液。

 

操作步骤

建议使用以下协议。 如果需要，可以调整方案以达到最佳效果。

设置PCR反应如下：
5 µL 10X聚合酶缓冲液（不含镁）
2.5 µL的50 mM MgCl2
2 µL 50X Q4ever Green工作溶液
2 µL的5 mM dUTP
1-5个单位的DNA聚合酶
加入所需的cDNA
每个引物100-1000 nM（正向和反向引物的终浓度）
用dH2O将最终体积调整为50 µL。
在热循环荧光计上执行实时PCR并记录荧光信号。

 

参考文献

Direct detection of Helicobacter pylori from biopsies of patients in Lagos, Nigeria using real-time PCR-a pilot study.
Authors: Ajayi, A and Jolaiya, T and Smith, S I
Journal: BMC research notes (2021): 90

MinION Nanopore-based detection of Clavibacter nebraskensis, the corn Goss’s wilt pathogen, and bacteriomic profiling of necrotic lesions of naturally-infected leaf samples.
Authors: Xu, Renlin and Adam, Lorne and Chapados, Julie and Soliman, Atta and Daayf, Fouad and Tambong, James T
Journal: PloS one (2021): e0245333

A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor.
Authors: Shi, Bing and Li, Yuanming and Wu, Di and Wu, Wenming
Journal: The Analyst (2020): 2767-2773

A quantitative loop-mediated isothermal amplification assay for detecting a novel goose astrovirus.
Authors: He, Dalin and Yang, Jing and Jiang, Xiaoning and Lin, Yun and Chen, Hao and Tang, Yi and Diao, Youxiang
Journal: Poultry science (2020): 6586-6592

A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase.
Authors: Purhonen, Janne and Banerjee, Rishi and McDonald, Allison E and Fellman, Vineta and Kallijärvi, Jukka
Journal: Nucleic acids research (2020): e87

Amplification Curve Analysis: Data-Driven Multiplexing Using Real-Time Digital PCR.
Authors: Moniri, Ahmad and Miglietta, Luca and Malpartida-Cardenas, Kenny and Pennisi, Ivana and Cacho-Soblechero, Miguel and Moser, Nicolas and Holmes, Alison and Georgiou, Pantelis and Rodriguez-Manzano, Jesus
Journal: Analytical chemistry (2020): 13134-13143

Comprehensive Data of P53 R282 Gene Mutation with Human Papillomaviruses (HPV)-Associated Oral Squamous Cell Carcinoma (OSCC).
Authors: Ekalaksananan, Tipaya and Wongjampa, Weerayut and Phusingha, Pensiri and Chuerduangphui, Jureeporn and Vatanasapt, Patravoot and Promthet, Supannee and Patarapadungkit, Natcha and Pientong, Chamsai
Journal: Pathology oncology research : POR (2020): 1191-1199

Detection of Phytophthora infestans by Loop-Mediated Isothermal Amplification, Real-Time LAMP, and Droplet Digital PCR.
Authors: Ristaino, Jean B and Saville, Amanda C and Paul, Rajesh and Cooper, Donald C and Wei, Qingshan
Journal: Plant disease (2020): 708-716

Detection of extended-spectrum beta-lactamase cefotaxime resistance and virulence genes in Escherichia coli by duplex quantitative real-time PCR and melt curve analysis.
Authors: Aijuka, M and Buys, E M
Journal: Letters in applied microbiology (2020): 54-60

Development of an EvaGreen based real-time RT-PCR assay for rapid detection, quantitation and diagnosis of goose calicivirus.
Authors: Lin, Su and Zhang, Shizhong and Wang, Shao and Xie, Kaichun and Jiang, Dandan and Xiao, Shifeng and Chen, Xiuqin and Chen, Shaoying
Journal: Molecular and cellular probes (2020): 101489

PE-iFluor 750 串联染料 货号2704-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

PE-iFluor 750 串联染料

PE-iFluor 750 串联染料

货号 2704 存储条件 在2-8度冷藏保存, 避免光照
规格 1 mg 价格 2388
Ex (nm) 566 Em (nm) 778
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：2704

产品名称：PE-iFluor 750 串联染料

规格：1mg

储存条件：2-8℃避光防潮

保质期：6个月

 

产品物理化学光谱特性

外观：固体

溶剂：水

激发波长(nm)：566

发射波长(nm)：778

 

产品介绍

PE-iFluor 750串联染料 是流式细胞术中使用的一种新颜色染料。其主要吸收峰位于 565 nm，发射峰位于 ~770 nm。它已通过光谱流式细胞仪进行了验证。 PE-iFluor 750 串联染料的染色指数比相应的 PE-Alexa Fluor 750 或 PE-Cy7 串联染料更高。 AAT Bioquest 为常规和光谱流式细胞术应用提供最多的颜色，包括 iFluor 、mFluor 有机染料及其各种串联染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的PE-iFluor 750 串联染料。 

点击查看光谱

 

参考文献

Fluorescent energy transfer causing misleading signal in multicolor flow cytometry.
Authors: Khenine, Hana and Waeckel, Louis and Seghrouchni, Fouad and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)

The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205

Probing Surface Membrane Receptors Using Engineered Bacteriophage Bioconjugates.
Authors: Lomakin, Yakov A and Kaminskaya, Alena N and Stepanov, Alexey V and Shmidt, Anna A and Gabibov, Alexander G and Belogurov, Alexey A
Journal: Bioconjugate chemistry (2019): 1500-1506

A ten-color tube with dried antibody reagents for the screening of hematological malignancies.
Authors: Correia, R P and Rajab, A and Bento, L C and Alexandre, A M and Vaz, A C and Schimidell, D and Pedro, E C and Perin, F S and Nozawa, S T and Barroso, R S and Bacal, N S
Journal: International journal of laboratory hematology (2018): 136-143

Initial flow cytometric evaluation of the Clearllab lymphoid screen.
Authors: Hedley, B D and Cheng, G and Luider, J and Kern, W and Lozanski, G and Chin-Yee, I and Lowes, L E and Keeney, M and Careaga, D and Magari, R and Tejidor, L
Journal: Cytometry. Part B, Clinical cytometry (2018): 707-713

Reduced CD4 T Lymphocytes in Lymph Nodes of the Mouse Model of Autism Induced by Valproic Acid.
Authors: Baronio, Diego and Bauer-Negrini, Guilherme and Castro, Kamila and Della-Flora Nunes, Gustavo and Riesgo, Rudimar and Mendes-da-Cruz, Daniella Arêas and Savino, Wilson and Gottfried, Carmem and Bambini-Junior, Victorio
Journal: Neuroimmunomodulation (2018): 280-284

CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.
Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080

Comparison of Flow-cytometric Antibody Secreting Cell Assay and Mabtech Immunoglobulin ELISpot Assay.
Authors: Lee, N and In, J W and Kim, H and Roh, E Y and Shin, S and Park, K U and Yang, J and Song, E Y
Journal: Transplantation proceedings (2017): 963-966

Semiconducting polymer dots with bright narrow-band emission at 800 nm for biological applications.
Authors: Chen, Dandan and Wu, I-Che and Liu, Zhihe and Tang, Ying and Chen, Haobin and Yu, Jiangbo and Wu, Changfeng and Chiu, Daniel T
Journal: Chemical science (2017): 3390-3398

[Changes of monocyte and monocyte-platelet aggregates in different subgroups of thrombotic events in patients with acute myocardial infarction during PCI].
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965

APC-iFluor 750 串联染料 货号2626-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

APC-iFluor 750 串联染料

APC-iFluor 750 串联染料

货号 2626 存储条件 在2-8度冷藏保存, 避免光照
规格 1 mg 价格 2388
Ex (nm) 754 Em (nm) 776
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：2626

产品名称：APC-iFluor 750 串联染料

规格：1mg

储存条件：2-8℃避光防潮

保质期：6个月

 

产品物理化学光谱特性

外观：固体

溶剂：水

 

产品介绍

串联染料是一类独特的荧光分子，由两种不同的共价连接荧光团、供体（例如 PE 或 APC）和发射较长波长的荧光受体（例如 Texas Red、Cy5、Cy7、iFluor 594 或 iFluor 750组成）。APC-iFluor 750 串联染料是常用 APC-Cy7 的绝佳替代品，具有更高的 FRET 效率和信号。其主要吸收峰位于 651 nm，发射峰位于 ~780 nm。 金畔还提供其预活化的 APC-iFluor 750 串联染料，以促进 APC-iFluor 750 串联与抗体和其他蛋白质（如链霉亲和素和其他二级试剂）的串联。我们的预活化 APC-iFluor 750 可随时进行缀合，其产量比传统繁琐的SMCC 的化学方法高得多。此外，我们的预活化 APC-iFluor 750 串联染料通过蛋白质中丰富的氨基与蛋白质缀合，而 SMCC 化学靶向必须通过抗体还原再生的硫醇基团。

 

参考文献

CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.
Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080

Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.
Authors: De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110

Analysis of Populations of Memory T-Helper Cells Expressing CXCR3 and CCR6 Chemokine Receptors in Peripheral Blood of Patients with Chronic Viral Hepatitis C.
Authors: Elezov, D S and Kudryavtsev, I V and Arsent’ev, N A and Basin, V V and Esaulenko, E V and Semenov, A V and Totolyan, A A
Journal: Bulletin of experimental biology and medicine (2015): 238-42

Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53

A flow cytometric method for the analysis of macrophages in the vascular wall.
Authors: Moore, Jeffrey P and Sakkal, Samy and Bullen, Michelle L and Kemp-Harper, Barbara K and Ricardo, Sharon D and Sobey, Christopher G and Drummond, Grant R
Journal: Journal of immunological methods (2013): 33-43

Combined normal donor and CLL: Single tube ZAP-70 analysis.
Authors: Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E
Journal: Cytometry. Part B, Clinical cytometry (2012): 67-77

The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.
Authors: Cannizzo, Elisa and Carulli, Giovanni and Del Vecchio, Luigi and Ottaviano, Virginia and Bellio, Emanuele and Zenari, Ezio and Azzarà, Antonio and Petrini, Mario and Preffer, Frederic
Journal: American journal of clinical pathology (2012): 377-86

Measurement conditions for flow cytometry analyses of cell lines from urological carcinomas.
Authors: Tölle, Angelika and Abdallah, Ziyad and Jung, Klaus and Bäumler, Hans
Journal: Journal of fluorescence (2010): 779-86

An optimized flow cytometry protocol for analysis of angiogenic monocytes and endothelial progenitor cells in peripheral blood.
Authors: Hristov, Mihail and Schmitz, Susanne and Schuhmann, Christoph and Leyendecker, Thorsten and von Hundelshausen, Philipp and Krötz, Florian and Sohn, Hae-Young and Nauwelaers, Frans A and Weber, Christian
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 848-53

Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90

APC-AF750 串联染料 货号2627-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

APC-AF750 串联染料

APC-AF750 串联染料

货号 2627 存储条件 在2-8度冷藏保存, 避免光照
规格 1 mg 价格 2388
Ex (nm) 754 Em (nm) 782
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：2627

产品名称：APC-AF750 串联染料

规格：1mg

储存条件：2-8℃避光防潮

保质期：6个月

 

产品物理化学光谱特性

外观：固体

溶剂：水

 

产品介绍

串联染料是一类独特的荧光分子，由两种不同的共价连接荧光团、供体（例如 PE 或 APC）和发射较长波长的荧光受体（例如 Texas Red、Cy5、Cy7、iFluor 594 或 iFluor 750组成）。APC-AF750 串联染料具有与常用 APC-Alexa Fluor® 750 相同的结构和特性。其主要吸收峰位于 651 nm，发射峰位于 ~780 nm。

 

参考文献

Comparison between photostability of Alexa Fluor 448 and Alexa Fluor 647 with conventional dyes FITC and APC by flow cytometry.
Authors: Rai, S and Bhardwaj, U and Misra, A and Singh, S and Gupta, R
Journal: International journal of laboratory hematology (2018): e52-e54

Dual-Color Fluorescence Imaging of EpCAM and EGFR in Breast Cancer Cells with a Bull’s Eye-Type Plasmonic Chip.
Authors: Izumi, Shota and Yamamura, Shohei and Hayashi, Naoko and Toma, Mana and Tawa, Keiko
Journal: Sensors (Basel, Switzerland) (2017)

[Changes of monocyte and monocyte-platelet aggregates in different subgroups of thrombotic events in patients with acute myocardial infarction during PCI].
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965

Antigen processing of glycoconjugate vaccines; the polysaccharide portion of the pneumococcal CRM(197) conjugate vaccine co-localizes with MHC II on the antigen processing cell surface.
Authors: Lai, Zengzu and Schreiber, John R
Journal: Vaccine (2009): 3137-44

Investigating the role of Natural Killer T-cells in Gram negative infections of patients with type 2 diabetes mellitus.
Authors: Karagianni, P and Polyzos, S A and Bougiouklis, D and Tsapas, A and Paletas, K
Journal: Hippokratia: 231-4

iFluor 597 琥珀酰亚胺酯 货号1050-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor 597 琥珀酰亚胺酯

iFluor 597 琥珀酰亚胺酯

货号 1050 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 3612
Ex (nm) 598 Em (nm) 618
分子量 1058.29 溶剂 DMSO
产品详细介绍

简要概述

iFluor 597琥珀酰亚胺酯是iFluor系列荧光标记染料之一，可以覆盖整个可见光谱。所有iFluor 染料都具有优异的水溶性。它们的亲水性使有机溶剂的使用极小化。与常规的染料(如FITC，TRITC，Texas Red ，Cy3 ，Cy5和Cy7)相比，iFluor 染料具有更好的标记性能。一些iFluor 染料在某些抗体上明显优于Alexa Fluor 标记染料。它们是用于标记蛋白质和核酸的极便宜的荧光染料（替代Alexa Fluor 染料）。每种iFluor染料的开发都与特定的Alexa Fluor 或其他标记染料（如DyLight 染料）的光谱特性相匹配。

琥珀酰亚胺基（NHS）酯被证明是用于胺修饰的极佳试剂，因为形成的酰胺键基本上与天然肽键相同并且稳定。这些试剂通常是稳定的并且与脂族胺显示出良好的反应性和选择性。当琥珀酰亚胺酯化合物用于缀合反应时，需要考虑的因素很少：1.溶剂：在大多数情况下，活性染料应溶于无水二甲基甲酰胺（DMF）或二甲基亚砜（DMSO）中。 2.反应pH：胺与琥珀酰亚胺酯的标记反应强烈依赖于pH。胺反应性试剂与非质子化脂族胺基团反应，包括蛋白质的末端胺和赖氨酸的β-氨基。因此，胺酰化反应通常在pH 7.5以上进行。通过琥珀酰亚胺酯进行的蛋白质修饰通常可以在pH 8.5-9.5下进行。 3.反应缓冲液：使用胺反应试剂时，必须避免使用含有游离胺（如Tris和甘氨酸）和硫醇化合物的缓冲液。广泛用于蛋白质沉淀的铵盐（例如硫酸铵和乙酸铵）也必须在进行染料缀合之前除去（例如通过透析）。 4.反应温度：大多数缀合在室温下进行。特定标记反应可能需要升高或降低的温度。iFluor系列染料是AF系列染料的完美替代品。

 

iFluor 597 琥珀酰亚胺酯 货号1050

  • AAT Bioquest iFluor 染料干货锦集 你想了解的都在这里
  • 锦囊：iFluor 系列染料大集合

产品说明书

染色样本分析

操作步骤

1.准备蛋白质储备溶液（溶液A）：

将100μL反应缓冲液（如1 M碳酸钠溶液或1 M磷酸盐缓冲液，pH~9.0）与900μL目标蛋白溶液（如抗体，蛋白质浓度> 2 mg / ml，如果可能）混合至100μL给予1 mL蛋白质标记原液。

注1：蛋白质溶液（溶液A）的pH值应为8.5±0.5。如果蛋白质溶液的pH低于8.0，则使用1M碳酸氢钠溶液或1M pH 9.0磷酸盐缓冲液将pH调节至8.0-9.0的范围。

注2：蛋白质应溶解于pH7.2-7.4的1X磷酸盐缓冲盐水（PBS）中。如果蛋白质溶解在Tris或甘氨酸缓冲液中，则必须用pH7.2-7.4的1X PBS透析，以除去广泛用于蛋白质沉淀的游离胺或铵盐（例如硫酸铵和乙酸铵）。

注3：不纯的抗体、稳定的牛血清蛋白（BSA）抗体或明胶不会被很好的标记。叠氮化钠或硫柳汞的存在也可能干扰缀合反应。可以通过透析或旋转柱除去叠氮化钠或硫柳汞，以获得极佳标记结果。

注4：如果蛋白质浓度低于2 mg / mL，则结合效率会显着降低。为获得极佳标记效率，建议极终蛋白质浓度范围为2-10 mg / mL。

 

2.准备染料储备溶液（溶液B）：

将无水DMSO加入到iFluor 染料SE小瓶中以制备10-20mM储备溶液。 通过移液或涡旋混合均匀。

注意：在开始缀合前准备染料储备溶液（溶液B）。 及时使用。 染料储备溶液的长期储存可降低染料活性。 溶液B可在冰箱中保存两周，避光保存。 避免冻融循环。

 

3.确定极佳染料/蛋白质比例（可选）：

注意：每种蛋白质都需要不同的染料/蛋白质比例，这也取决于染料的性质。蛋白质的过度标记可能不利地影响其结合亲和力，而低染料/蛋白质比率的蛋白质缀合物会降低灵敏度。我们建议您通过使用连续不同量的标记染料溶液重复步骤4和5来实验确定极佳染料/蛋白质比率。通常，对于大多数染料 – 蛋白质缀合物，推荐使用4-6种染料/蛋白质。

3.1使用10：1摩尔比的溶液B（染料）/溶液A（蛋白质）作为起始点：将5μl染料储备溶液（溶液B，假设染料储备溶液为10 mM）加入到样品瓶中。蛋白质溶液（95μl溶液A）有效摇动。假设蛋白质浓度为10mg / mL并且蛋白质的分子量为~200KD，蛋白质的浓度为~0.05mM。

注意：蛋白质溶液中DMSO的浓度应<10％。

3.2运行缀合反应（参见下面的步骤4）。

3.3重复＃3.2，溶液B /溶液A的摩尔比为5：1;分别为15：1和20：1。

3.4使用预制的旋转柱纯化所需的缀合物。

3.5计算上述4种结合物的染料/蛋白质比（DOS）（见说明书）。

3.6运行上述4种结合物的功能测试，确定极佳的染料/蛋白质比例，以扩大标记反应。

 

4.运行结合反应：

4.1有效加入适量的染料储备溶液（溶液B）到蛋白质溶液（溶液A）的小瓶中晃动。

注意：溶液B /溶液的极佳摩尔比由步骤3.6确定。如果跳过步骤3，我们建议使用10：1溶液B（染料）/溶液A（蛋白质）的摩尔比。

4.2继续在室温下旋转或摇动反应混合物30-60分钟。

 

5.纯化缀合物

以下方案是使用Sephadex G-25柱纯化染料 – 蛋白质缀合物的实例。

5.1按照制造说明准备Sephadex G-25色谱柱。

5.2将反应混合物（直接从步骤4）加载到Sephadex G-25柱的顶部。

5.3样品在顶部树脂表面下方运行时立即加入PBS（pH 7.2-7.4）。

5.4向所需样品中加入更多PBS（pH 7.2-7.4）以完成色谱柱纯化。 合并含有所需染料 – 蛋白质缀合物的级分。

注1：立即使用时，染料 – 蛋白质偶联物需要用染色缓冲液稀释，并等分多次使用。

注2：对于长期储存，染料 – 蛋白质缀合物溶液需要浓缩或冷冻干燥

 

参考文献

A fully integrated isotachophoresis with a programmable microfluidic platform.
Authors: Shebindu, Adam and Somaweera, Himali and Estlack, Zachary and Kim, Jungtae and Kim, Jungkyu
Journal: Talanta (2021): 122039

Comparison of Sensory and Motor Innervation Between the Acupoints LR3 and LR8 in the Rat With Regional Anatomy and Neural Tract Tracing.
Authors: Xu, Dongsheng and Zou, Ling and Zhang, Wenjie and Liao, Jieying and Wang, Jia and Cui, Jingjing and Su, Yuxin and Wang, Yuqing and Guo, Yating and Shen, Yi and Bai, Wanzhu
Journal: Frontiers in integrative neuroscience (2021): 728747

Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).
Authors: Seo, Hyesu and Kim, Kyunghee and Park, Woong June
Journal: Biomolecules (2021)

KFP-1, a Novel Calcium-Binding Peptide Isolated from Kefir, Promotes Calcium Influx Through TRPV6 Channels.
Authors: Chang, Gary Ro-Lin and Tu, Min-Yu and Chen, Yu-Hsuan and Chang, Ku-Yi and Chen, Chien-Fu and Lai, Jen-Chieh and Tung, Yu-Tang and Chen, Hsiao-Ling and Fan, Hueng-Chuen and Chen, Chuan-Mu
Journal: Molecular nutrition & food research (2021): e2100182

Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides.
Authors: Iwata, Takahiro and Hirose, Hisaaki and Sakamoto, Kentarou and Hirai, Yusuke and Arafiles, Jan Vincent V and Akishiba, Misao and Imanishi, Miki and Futaki, Shiroh
Journal: Angewandte Chemie (International ed. in English) (2021)

MicroRNA-126 inhibits pathological retinal neovascularization via suppressing vascular endothelial growth factor expression in a rat model of retinopathy of prematurity.
Authors: Fan, Yuan-Yao and Liu, Chi-Hsien and Wu, An-Lun and Chen, Hung-Chi and Hsueh, Yi-Jen and Chen, Kuan-Jen and Lai, Chi-Chun and Huang, Chung-Ying and Wu, Wei-Chi
Journal: European journal of pharmacology (2021): 174035

Retinal ganglion cells projecting to superior colliculus and pulvinar in marmoset.
Authors: Grünert, Ulrike and Lee, Sammy C S and Kwan, William C and Mundinano, Inaki-Carril and Bourne, James A and Martin, Paul R
Journal: Brain structure & function (2021)

Fluorescently-labeled fremanezumab is distributed to sensory and autonomic ganglia and the dura but not to the brain of rats with uncompromised blood brain barrier.
Authors: Noseda, Rodrigo and Schain, Aaron J and Melo-Carrillo, Agustin and Tien, Jason and Stratton, Jennifer and Mai, Fanny and Strassman, Andrew M and Burstein, Rami
Journal: Cephalalgia : an international journal of headache (2020): 229-240

Modeling iontophoretic drug delivery in a microfluidic device.
Authors: Moarefian, Maryam and Davalos, Rafael V and Tafti, Danesh K and Achenie, Luke E and Jones, Caroline N
Journal: Lab on a chip (2020): 3310-3321

Cell-based immunofluorescence assay for screening the neurogenesis potential of new drugs in adult hippocampal neural progenitor cells.
Authors: Zhang, Kun and Li, Bin and Li, Peifang and Yang, Xiaoli and Cui, Huixian and Liu, Xiaoyun
Journal: Acta neurobiologiae experimentalis (2019): 302-308

 

相关产品

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iFluor 594马来酰亚胺 Cat#1064

TAMRA 染料 qPCR 校准板*针对 ABI7500 快速 96 孔进行了优化* 货号67000-AAT Bioquest荧光染料

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TAMRA 染料 qPCR 校准板*针对 ABI7500 快速 96 孔进行了优化*

TAMRA 染料 qPCR 校准板*针对 ABI7500 快速 96 孔进行了优化*

TAMRA 染料 qPCR 校准板*针对 ABI7500 快速 96 孔进行了优化* 货号67000 货号 67000 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

TAMRA 染料 qPCR 校准板可用于校准和维护配备快速 96 孔模块的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。 

 

参考文献

Development and validation of a new 18 X-STR typing assay for forensic applications.
Authors: Zhang, Yinming and Yu, Zhengliang and Mo, Xiaoting and Zhao, Xingchun and Li, Wanshui and Liu, Hong and Liu, Chao and Wu, Riga and Sun, Hongyu
Journal: Electrophoresis (2021): 766-773
[Mesenchymal stem cells derived apoptotic extracellular vesicles attenuate pro-inflammatory macrophages induced by Porphyromonas gingivalis lipopolysaccharide].
Authors: Ye, Q Y and Li, Z H and Wang, Y Z and Liu, S Y and Zhou, J and Liu, S Y and Wang, Q T
Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (2021): 791-798
MS2 device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device.
Authors: Wu, Xiaosong and Pan, Jingyu and Zhu, Xinchao and Hong, Chenggang and Hu, Anzhong and Zhu, Cancan and Liu, Yong and Yang, Ke and Zhu, Ling
Journal: The Analyst (2021): 3823-3833
The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027
Double-Stranded DNA Fragments Bearing Unrepairable Lesions and Their Internalization into Mouse Krebs-2 Carcinoma Cells.
Authors: Dolgova, Evgeniya V and Evdokimov, Alexey N and Proskurina, Anastasia S and Efremov, Yaroslav R and Bayborodin, Sergey I and Potter, Ekaterina A and Popov, Alexey A and Petruseva, Irina O and Lavrik, Olga I and Bogachev, Sergey S
Journal: Nucleic acid therapeutics (2019): 278-290
Design and anti-tumor activity of self-loaded nanocarriers of siRNA.
Authors: Han, Wenzhao and Yuan, Ye and Li, Hui and Fu, Zhendong and Wang, Mingyang and Guan, Shuwen and Wang, Liping
Journal: Colloids and surfaces. B, Biointerfaces (2019): 110385
The mechanism and regularity of quenching the effect of bases on fluorophores: the base-quenched probe method.
Authors: Mao, Huihui and Luo, Guanghua and Zhan, Yuxia and Zhang, Jun and Yao, Shuang and Yu, Yang
Journal: The Analyst (2018): 3292-3301
Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells.
Authors: Potter, Ekaterina A and Dolgova, Evgenia V and Proskurina, Anastasia S and Efremov, Yaroslav R and Minkevich, Alexandra M and Rozanov, Aleksey S and Peltek, Sergey E and Nikolin, Valeriy P and Popova, Nelly A and Seledtsov, Igor A and Molodtsov, Vladimir V and Zavyalov, Evgeniy L and Taranov, Oleg S and Baiborodin, Sergey I and Ostanin, Alexander A and Chernykh, Elena R and Kolchanov, Nikolay A and Bogachev, Sergey S
Journal: Oncotarget (2017): 9425-9441
Detection of Yersinia Enterocolitica Species in Pig Tonsils and Raw Pork Meat by the Real-Time Pcr and Culture Methods.
Authors: Stachelska, M A
Journal: Polish journal of veterinary sciences (2017): 477-484
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20

VIC 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67002-AAT Bioquest荧光染料

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VIC 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

VIC 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

VIC 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67002 货号 67002 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

VIC 染料 qPCR 校准板可用于维护您的 7500 实时 PCR 系统和快速 96 孔板。 对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。 该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。 

 

参考文献

Rapid screening method of Saccharomyces cerevisiae mutants using calcofluor white and aniline blue.
Authors: Perrine-Walker, Francine and Payne, Jennifer
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2021): 1077-1086
The P2X7 receptor antagonist JNJ-47965567 administered thrice weekly from disease onset does not alter progression of amyotrophic lateral sclerosis in SOD1G93A mice.
Authors: Ly, Diane and Dongol, Anjila and Cuthbertson, Peter and Guy, Thomas V and Geraghty, Nicholas J and Sophocleous, Reece A and Sin, Lucia and Turner, Bradley J and Watson, Debbie and Yerbury, Justin J and Sluyter, Ronald
Journal: Purinergic signalling (2020): 109-122
Development of the MitoQ assay as a real-time quantification of mitochondrial DNA in degraded samples.
Authors: Wai, Ka Tak and Gunn, Peter and Barash, Mark
Journal: International journal of legal medicine (2019): 411-417
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Lineage-specific detection of influenza B virus using real-time polymerase chain reaction with melting curve analysis.
Authors: Tewawong, Nipaporn and Chansaenroj, Jira and Klinfueng, Sirapa and Vichiwattana, Preeyaporn and Korkong, Sumeth and Thongmee, Thanunrat and Theamboonlers, Apiradee and Payungporn, Sunchai and Vongpunsawad, Sompong and Poovorawan, Yong
Journal: Archives of virology (2016): 1425-35
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
The effects of escitalopram on myocardial apoptosis and the expression of Bax and Bcl-2 during myocardial ischemia/reperfusion in a model of rats with depression.
Authors: Wang, Yiming and Zhang, Hongming and Chai, Fangxian and Liu, Xingde and Berk, Michael
Journal: BMC psychiatry (2014): 349
Transcriptional regulators Myb and BCL11A interplay with DNA methyltransferase 1 in developmental silencing of embryonic and fetal β-like globin genes.
Authors: Roosjen, Mark and McColl, Bradley and Kao, Betty and Gearing, Linden J and Blewitt, Marnie E and Vadolas, Jim
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014): 1610-20
Identification of an isogenic semidwarf rice cultivar carrying the Green Revolution sd1 gene by multiplex codominant ASP-PCR and SSR markers.
Authors: Naito, Yoshiki and Tomita, Motonori
Journal: Biochemical genetics (2013): 530-42

ROX 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67004-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ROX 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

ROX 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

ROX 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67004 货号 67004 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

ROX 染料 qPCR 校准板可用于维护配备 Fast 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。 该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。 

 

参考文献

SARS-CoV-2 Detection using Real Time PCR by a Commercial Diagnostic Kit.
Authors: Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A
Journal: Mymensingh medical journal : MMJ (2020): 596-600
RT-qPCR Detection of Low-Copy HIV RNA with Yin-Yang Probes.
Authors: Kireev, Dmitry E and Farzan, Valentina M and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 27-35
The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027
Developmental validation of the Microreader™ 20A ID system.
Authors: Qu, Shengqiu and Li, Hang and Li, Yifan and Lv, Meili and Yang, Fan and Zhu, Jing and Yu, Zailiang and Liu, Yuqing and Chen, Chuguang and Wang, Yinji and Li, Zhuo and Zhang, Lin and Liang, Weibo
Journal: Electrophoresis (2019): 3099-3107
Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.
Authors: Aviv, Gili and Gal-Mor, Ohad
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 23-32
Detection of simultaneous multi-mutations using base-quenched probe.
Authors: Mao, Huihui and Luo, Guanghua and Zhang, Jun and Xu, Ning
Journal: Analytical biochemistry (2018): 79-81
Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Sun, Y and Qiao, B and Si, C C and Hu, P and Lu, S Y and Ren, H L and Liu, Z S and Qiu, H J and Liu, J Q
Journal: Biosensors & bioelectronics (2016): 194-199
Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701
Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67006-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67006 货号 67006 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

FAM 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。 该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

Novel design of nucleic acid standards for hydrolysis probe-based PCR with melting analysis.
Authors: Baoutina, Anna and Bhat, Somanath
Journal: Gene therapy (2021)
Determination of Advantages and Limitations of qPCR Duplexing in a Single Fluorescent Channel.
Authors: Zhang, Haoqing and Yan, Zhiqiang and Wang, Xinlu and Gaňová, Martina and Chang, Honglong and Laššáková, Soňa and Korabecna, Marie and Neuzil, Pavel
Journal: ACS omega (2021): 22292-22300
SARS-CoV-2 Detection using Real Time PCR by a Commercial Diagnostic Kit.
Authors: Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A
Journal: Mymensingh medical journal : MMJ (2020): 596-600
Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity.
Authors: Menendez-Gil, Pilar and Caballero, Carlos J and Solano, Cristina and Toledo-Arana, Alejandro
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 41-58
Development and Evaluation of a Single Dye Duplex Droplet Digital PCR Assay for the Rapid Detection and Quantification of Mycobacterium tuberculosis.
Authors: Nyaruaba, Raphael and Xiong, Jin and Mwaliko, Caroline and Wang, Nuo and Kibii, Belindah J and Yu, Junping and Wei, Hongping
Journal: Microorganisms (2020)
A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor.
Authors: Shi, Bing and Li, Yuanming and Wu, Di and Wu, Wenming
Journal: The Analyst (2020): 2767-2773
Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions.
Authors: Tomé, Stéphanie and Gourdon, Geneviève
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 11-23
Ratiometric fluorescence sensor based on carbon dots as internal reference signal and T7 exonuclease-assisted signal amplification strategy for microRNA-21 detection.
Authors: Wang, Zhenzhen and Xue, Zhiqiang and Hao, Xiaoli and Miao, Chenfang and Zhang, Jianzhong and Zheng, Yanjie and Zheng, Zongfu and Lin, Xinhua and Weng, Shaohuang
Journal: Analytica chimica acta (2020): 212-219
PharmFrag: An Easy and Fast Multiplex Pharmacogenetics Assay to Simultaneously Analyze 9 Genetic Polymorphisms Involved in Response Variability of Anticancer Drugs.
Authors: Bouvet, Régis and Verdier, Marie-Clémence and El Baroudi, Yahya and Galibert, Marie-Dominique and David, Véronique and Schutz, Sacha and Tron, Camille
Journal: International journal of molecular sciences (2020)
The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67008-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67008 货号 67008 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

SYBR 染料 qPCR 校准板可用于维护配备 Fast 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。 该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

Combined evaluation of proliferation and apoptosis to calculate IC50 of VPA-induced PANC-1 cells and assessing its effect on the Wnt signaling pathway.
Authors: Ekici, Yeliz and Yilmaz, Abdullah and Kucuksezer, Umut Can and Gazioglu, Sema Bilgic and Yamalioglu, Zeynep Dogusan and Gurol, Ali Osman and Linn, Thomas and Tuncer, Feyza Nur
Journal: Medical oncology (Northwood, London, England) (2021): 109
Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays.
Authors: Bonelli, Piero and Dei Giudici, Silvia and Peruzzu, Angela and Mura, Lorena and Santucciu, Cinzia and Maestrale, Caterina and Masala, Giovanna
Journal: Pathogens (Basel, Switzerland) (2021)
Expression levels of serum circulating microRNAs in pediatric patients with ventricular and supraventricular arrhythmias.
Authors: Moric-Janiszewska, Ewa and Smolik, Sławomir and Morka, Aleksandra and Szydłowski, Lesław and Kapral, Małgorzata
Journal: Advances in medical sciences (2021): 411-417
RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors.
Authors: Munawar, Mustafa Ahmad and Martin, Frank and Toljamo, Anna and Kokko, Harri and Oksanen, Elina
Journal: BioTechniques (2020): 270-280
Rapid detection of porcine DNA in processed food samples using a streamlined DNA extraction method combined with the SYBR Green real-time PCR assay.
Authors: Tan, Lee Lee and Ahmed, Siti Aminah and Ng, Siew Kit and Citartan, Marimuthu and Raabe, Carsten A and Rozhdestvensky, Timofey S and Tang, Thean Hock
Journal: Food chemistry (2020): 125654
Expression of SARS-CoV-2 receptor ACE2 and TMPRSS2 in human primary conjunctival and pterygium cell lines and in mouse cornea.
Authors: Ma, Di and Chen, Chong-Bo and Jhanji, Vishal and Xu, Ciyan and Yuan, Xiang-Ling and Liang, Jia-Jian and Huang, Yuqiang and Cen, Ling-Ping and Ng, Tsz Kin
Journal: Eye (London, England) (2020): 1212-1219
Preoperative heat shock protein 47 levels identify colorectal cancer patients with lymph node metastasis and poor prognosis.
Authors: Mori, Koichiro and Toiyama, Yuji and Okugawa, Yoshinaga and Ichikawa, Takashi and Nagano, Yuka and Oki, Satoshi and Shimura, Tadanobu and Fujikawa, Hiroyuki and Hiro, Junichiro and Kobayash, Minako and Araki, Toshimitsu and Inoue, Yasuhiro and Mohri, Yasuhiko and Kusunoki, Masato
Journal: Oncology letters (2020): 333
Distribution of virulence genes in bacteremic methicillin-resistant Staphylococcus aureus isolates from various sources.
Authors: Wang, Fu-Der and Wu, Ping-Feng and Chen, Su-Jung
Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (2019): 426-432
Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance.
Authors: Saeed, Noor AlHuda Ali A H and Hamzah, Israa Hussein and Al-Gharrawi, Samar Abdul Raheem
Journal: BMC research notes (2019): 455
Aberrant Expression of the miR-181b/miR-222 after Hematopoietic Stem Cell Transplantation in Patients with Acute Myeloid Leukemia.
Authors: Iravani Saadi, Mahdiyar and Arandi, Nargess and Yaghobi, Ramin and Azarpira, Negar and Geramizadeh, Bita and Ramzi, Mani
Journal: Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion (2019): 446-450

NED 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67010-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

NED 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

NED 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

NED 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67010 货号 67010 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

NED 染料 qPCR 校准板可用于维护您的 7500 实时 PCR 系统和快速 96 孔板。 对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。 该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。 

 

参考文献

DNA degradation in human teeth exposed to thermal stress.
Authors: Lozano-Peral, Diego and Rubio, Leticia and Santos, Ignacio and Gaitán, María Jesús and Viguera, Enrique and Martín-de-Las-Heras, Stella
Journal: Scientific reports (2021): 12118
Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
An improved rapid quantitative detection and identification method for a wide range of fungi.
Authors: Soeta, Nobutoshi and Terashima, Masanori and Gotoh, Mitsukazu and Mori, Shuichi and Nishiyama, Kyoko and Ishioka, Ken and Kaneko, Hisatoshi and Suzutani, Tatsuo
Journal: Journal of medical microbiology (2009): 1037-1044
Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK.
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36
TaqMan reverse transcriptase-polymerase chain reaction coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type.
Authors: Luthra, Rajyalakshmi and Medeiros, L Jeffrey
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 135-45
TaqMan RT-PCR assay coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type.
Authors: Luthra, Rajyalakshmi and Sanchez-Vega, Beatriz and Medeiros, L Jeffrey
Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (2004): 96-103
Development of a 17-plex microsatellite polymerase chain reaction kit for genotyping horses.
Authors: Dimsoski, Pero
Journal: Croatian medical journal (2003): 332-5
Semiquantitative and qualitative assessment of B-lymphocyte V H repertoire by a fluorescent multiplex PCR.
Authors: Feuchtenberger, Martin and Tony, Hans-Peter and Rouzière, Anne-Sophie and Jacobi, Anette and Dörner, Thomas and Kneitz, Christian and Starostik, Petr
Journal: Journal of immunological methods (2003): 121-7

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67012-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67012 货号 67012 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

JOE 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。 该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027
Detection of simultaneous multi-mutations using base-quenched probe.
Authors: Mao, Huihui and Luo, Guanghua and Zhang, Jun and Xu, Ning
Journal: Analytical biochemistry (2018): 79-81
Molecular beacons with JOE dye: Influence of linker and 3′ couple quencher.
Authors: Tsybulsky, Dmitry A and Kvach, Maksim V and Ryazantsev, Dmitry Y and Aparin, Ilya O and Stakheev, Alexander A and Prokhorenko, Igor A and Martynenko, Yury V and Gontarev, Sergey V and Formanovsky, Andrey A and Zatsepin, Timofei S and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Molecular and cellular probes (2016): 285-290
Mineralization Effect of Hyaluronan on Dental Pulp Cells via CD44.
Authors: Chen, Kuan-Liang and Yeh, Ying-Yi and Lung, Jrhau and Yang, Yu-Chi and Yuan, Kuo
Journal: Journal of endodontics (2016): 711-6
Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay.
Authors: Maksyutov, Rinat A and Gavrilova, Elena V and Shchelkunov, Sergei N
Journal: Journal of virological methods (2016): 215-220
Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes.
Authors: Skoblov, Aleksander Yu and Vichuzhanin, Maxim V and Farzan, Valentina M and Veselova, Olga A and Konovalova, Tatiana A and Podkolzin, Alexander T and Shipulin, German A and Zatsepin, Timofei S
Journal: Bioorganic & medicinal chemistry (2015): 6749-56
Combined effects of simvastatin and enamel matrix derivative on odontoblastic differentiation of human dental pulp cells.
Authors: Karanxha, Lorena and Park, Su-Jung and Son, Won-Jun and Nör, Jacques E and Min, Kyung-San
Journal: Journal of endodontics (2013): 76-82
In vivo quantitative evaluation of live and dead bacteria in root canal infection by using propidium monoazide with real-time PCR.
Authors: Kim, Sin-Young and Shin, Yooseok and Lee, Chan-Young and Jung, Il-Young
Journal: Journal of endodontics (2013): 1359-63
Two-dye and one- or two-quencher DNA probes for real-time PCR assay: synthesis and comparison with a TaqMan™ probe.
Authors: Ryazantsev, Dmitry Y and Tsybulsky, Dmitry A and Prokhorenko, Igor A and Kvach, Maksim V and Martynenko, Yury V and Philipchenko, Pavel M and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Analytical and bioanalytical chemistry (2012): 59-68
ADAM28 manipulates proliferation, differentiation, and apoptosis of human dental pulp stem cells.
Authors: Zhao, Zheng and Liu, Hongchen and Wang, Dongsheng
Journal: Journal of endodontics (2011): 332-9

7 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67020-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

7 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

7 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

7 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67020 货号 67020 存储条件 在零下15度以下保存, 避免光照
规格 1 Set 价格 11400
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

7 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。 该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

Evaluation of the Thermo Scientific SureTect Salmonella Species PCR Assay in a Broad Range of Foods and Select Environmental Surfaces: Pre-Collaborative and Collaborative Study: First Action 2021.02.
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Leonte, Ana-Maria and Vandoros, Evangelos J and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022): 167-190
Development and Evaluation of a TaqMan Real-Time PCR Assay for the Rapid Detection of Cross-Contamination of RD (Human) and L20B (Mouse) Cell Lines Used in Poliovirus Surveillance.
Authors: Ahmad, Ausaf and Lee, Joo R and Metz, John M and Tang, Xiaoling and Lin, Seh-Ching and Bagarozzi, Dennis A and Petway, David and Herzegh, Owen
Journal: Journal of virological methods (2022): 114354
Evaluation of the Thermo ScientificTM SureTectTMListeria monocytogenes PCR Assay in a Broad Range of Foods and Selected Environmental Surfaces: Pre-Collaborative and Collaborative Study, First Action 2021.05.
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Vandoros, Evangelos J and Leonte, Ana-Maria and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022)
The Comparison of Real-time-PCR-HRM and Microscopy Methods for Detection of Mixed Plasmodium spp. Infections in Laghman Province, Afghanistan.
Authors: Dalimi, Abdolhossein and Mosawi, Sayed Hussain
Journal: Infectious disorders drug targets (2021): 399-404
Clinical evaluation of a multiplex real-time RT-PCR assay for detection of SARS-CoV-2 in individual and pooled upper respiratory tract samples.
Authors: Laverack, Melissa and Tallmadge, Rebecca L and Venugopalan, Roopa and Cronk, Brittany and Zhang, XiuLin and Rauh, Rolf and Saunders, Amy and Nelson, William M and Plocharczyk, Elizabeth and Diel, Diego G
Journal: Archives of virology (2021): 2551-2561
TaqMan real time PCR for the Detection of the Gilbert’s Syndrome Markers UGT1A1*28; UGT1A1*36 and UGT1A1*37.
Authors: Daprà, Valentina and Alliaudi, Carla and Galliano, Ilaria and Dini, Maddalena and Curcio, Giada Lo and Calvi, Cristina and Archetti, Marialaura and Gavatorta, Martina and Bergallo, Massimiliano
Journal: Molecular biology reports (2021): 4953-4959
Rapid and Safe Detection of SARS-CoV-2 and Influenza Virus RNA Using Onsite Quantitative PCR Diagnostic Testing From Clinical Specimens Collected in Molecular Transport Medium.
Authors: Daum, Luke T and Fischer, Gerald W
Journal: The journal of applied laboratory medicine (2021): 1409-1416
Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay.
Authors: Potter, Robert F and Abro, Brooj and Eby, Charles S and Burnham, Carey-Ann D and Anderson, Neil W and Parikh, Bijal A
Journal: Diagnostic microbiology and infectious disease (2021): 115387
Validation of the Thermo Scientific™ SureTect™ Staphylococcus aureus PCR Assay for the Detection of Staphylococcus aureus in Dairy Matrices: AOAC Performance Tested MethodsSM 052101.
Authors: Evans, Katharine and Faulds, Nikki and Crabtree, David and Hughes, Annette and Sohier, Daniele and Manthe, Craig and Hahs, Matthew and Heikkinen, Pauliina and Hurskainen, Emmi and Koch, Kateland and Thompson, Wesley and Bastin, Benjamin and Benzinger, M Joseph
Journal: Journal of AOAC International (2021)
Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2.
Authors: Moore, Nicholas M and Li, Haiying and Schejbal, Debra and Lindsley, Jennifer and Hayden, Mary K
Journal: Journal of clinical microbiology (2020)

Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67014-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67014 货号 67014 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号：67014

产品名称：Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

规格：1 Plate

储存条件：保存在冰箱-15℃干燥

保质期：12个月

 

产品物理化学光谱特性

外观：液体

溶剂：DMSO

 

产品介绍

Cy3.5 Dye qPCR 校准板可用于维护您的 7500 Real-Time PCR 系统和快速 96 孔模块。对于大多数 qPCR 仪器，必须至少每六个月校准一次设备。该校准板无需任何额外的准备步骤即可使用。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来显着改善多路复用的 qPCR 结果。有关详细的校准操作，请参阅您的仪器指南。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cy3.5 染料 qPCR 校准板。

  

参考文献

Development of a rapid qPCR method to quantify lactic acid bacteria in cold-smoked salmon.
Authors: Jérôme, Marc and Passerini, Delphine and Chevalier, Frédérique and Marchand, Laetitia and Leroi, Françoise and Macé, Sabrina
Journal: International journal of food microbiology (2022): 109504
Semi-Quantitative Detection of Drosophila suzukii (Diptera: Drosophilidae) From Bulk Trap Samples Using PCR Technology.
Authors: Renkema, Justin M and McFadden-Smith, Wendy and Chen, Shu
Journal: Journal of economic entomology (2022)
Continuous polymerase chain reaction microfluidics integrated with a gold-capped nanoslit sensing chip for Epstein-Barr virus detection.
Authors: Hsieh, Han-Yun and Chang, Ray and Huang, Yung-Yu and Juan, Po-Han and Tahara, Hidetoshi and Lee, Kuan-Yi and Vo, Di Ngoc Kha and Tsai, Ming-Han and Wei, Pei-Kuen and Sheen, Horn-Jiunn and Fan, Yu-Jui
Journal: Biosensors & bioelectronics (2022): 113672
XCL1 Aggravates Diabetic Nephropathy-Mediated Renal Glomerular Endothelial Cell Apoptosis and Inflammatory Response via Regulating p53/Nuclear Factor-Kappa B Pathway.
Authors: Zhang, Yuan and Chen, Xiaolan and Fan, Yaping and Liu, Jing and Yuan, Li
Journal: Nephron (2022): 84-98
DNA-based quantification of Fusarium oxysporum f. sp. vasinfectum in environmental soils to describe spatial variation in inoculum density.
Authors: Davis, Roy and Isakeit, Thomas and Chappell, Thomas
Journal: Plant disease (2022)
First case of SARS-CoV-2 RNA detection in municipal solid waste leachate from Brazil.
Authors: Mondelli, Giulliana and Silva, Ednei Rodrigues and Claro, Ieda Carolina Mantovani and Augusto, Matheus Ribeiro and Duran, Adriana Feliciano Alves and Cabral, Aline Diniz and de Moraes Bomediano Camillo, Lívia and Dos Santos Oliveira, Luísa Helena and de Freitas Bueno, Rodrigo
Journal: The Science of the total environment (2022): 153927
Precise RNA Quantification by Counting Individual RNA Molecules Using High-Sensitivity Capillary Flow Cytometry.
Authors: Yoo, Hee-Bong and Park, Sang-Ryoul and Hong, Kee-Suk and Yang, Inchul
Journal: Analytical chemistry (2022): 1752-1759
Accurate qPCR quantification in polymicrobial communities requires assessment of gDNA extraction efficiency.
Authors: Cerca, Nuno and Lima, Ângela and França, Angela
Journal: Journal of microbiological methods (2022): 106421
Lentiviral standards to determine the sensitivity of assays that quantify lentiviral vector copy numbers and genomic insertion sites in cells.
Authors: Corre, Guillaume and Seye, Ababacar and Frin, Sophie and Ferrand, Maxime and Winkler, Kathrin and Luc, Cyril and Dorange, Fabien and Rocca, Céline J and Galy, Anne
Journal: Gene therapy (2022)
One-Step Multiplexed Droplet Digital Polymerase Chain Reaction for Quantification of p190 BCR-ABL1 Fusion Transcript in B-Lymphoblastic Leukemia.
Authors: Martinez, Ryan J and Kang, Qing and Nennig, Davis and Bailey, Nathanael G and Brown, Noah A and Betz, Bryan L and Tewari, Muneesh and Thyagarajan, Bharat and Bachanova, Veronika and Mroz, Pawel
Journal: Archives of pathology & laboratory medicine (2022): 92-100