TAMRA 染料 qPCR 校准板*针对 ABI7500 快速 96 孔进行了优化* 货号67000-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAMRA 染料 qPCR 校准板*针对 ABI7500 快速 96 孔进行了优化*

TAMRA 染料 qPCR 校准板*针对 ABI7500 快速 96 孔进行了优化*

TAMRA 染料 qPCR 校准板*针对 ABI7500 快速 96 孔进行了优化*    货号67000 货号 67000 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

TAMRA 染料 qPCR 校准板可用于校准和维护配备快速 96 孔模块的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器,必要的校准应至少每六个月进行一次。该校准板可以立即使用,无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。 

 

参考文献

Development and validation of a new 18 X-STR typing assay for forensic applications.
Authors: Zhang, Yinming and Yu, Zhengliang and Mo, Xiaoting and Zhao, Xingchun and Li, Wanshui and Liu, Hong and Liu, Chao and Wu, Riga and Sun, Hongyu
Journal: Electrophoresis (2021): 766-773
[Mesenchymal stem cells derived apoptotic extracellular vesicles attenuate pro-inflammatory macrophages induced by Porphyromonas gingivalis lipopolysaccharide].
Authors: Ye, Q Y and Li, Z H and Wang, Y Z and Liu, S Y and Zhou, J and Liu, S Y and Wang, Q T
Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (2021): 791-798
MS2 device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device.
Authors: Wu, Xiaosong and Pan, Jingyu and Zhu, Xinchao and Hong, Chenggang and Hu, Anzhong and Zhu, Cancan and Liu, Yong and Yang, Ke and Zhu, Ling
Journal: The Analyst (2021): 3823-3833
The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027
Double-Stranded DNA Fragments Bearing Unrepairable Lesions and Their Internalization into Mouse Krebs-2 Carcinoma Cells.
Authors: Dolgova, Evgeniya V and Evdokimov, Alexey N and Proskurina, Anastasia S and Efremov, Yaroslav R and Bayborodin, Sergey I and Potter, Ekaterina A and Popov, Alexey A and Petruseva, Irina O and Lavrik, Olga I and Bogachev, Sergey S
Journal: Nucleic acid therapeutics (2019): 278-290
Design and anti-tumor activity of self-loaded nanocarriers of siRNA.
Authors: Han, Wenzhao and Yuan, Ye and Li, Hui and Fu, Zhendong and Wang, Mingyang and Guan, Shuwen and Wang, Liping
Journal: Colloids and surfaces. B, Biointerfaces (2019): 110385
The mechanism and regularity of quenching the effect of bases on fluorophores: the base-quenched probe method.
Authors: Mao, Huihui and Luo, Guanghua and Zhan, Yuxia and Zhang, Jun and Yao, Shuang and Yu, Yang
Journal: The Analyst (2018): 3292-3301
Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells.
Authors: Potter, Ekaterina A and Dolgova, Evgenia V and Proskurina, Anastasia S and Efremov, Yaroslav R and Minkevich, Alexandra M and Rozanov, Aleksey S and Peltek, Sergey E and Nikolin, Valeriy P and Popova, Nelly A and Seledtsov, Igor A and Molodtsov, Vladimir V and Zavyalov, Evgeniy L and Taranov, Oleg S and Baiborodin, Sergey I and Ostanin, Alexander A and Chernykh, Elena R and Kolchanov, Nikolay A and Bogachev, Sergey S
Journal: Oncotarget (2017): 9425-9441
Detection of Yersinia Enterocolitica Species in Pig Tonsils and Raw Pork Meat by the Real-Time Pcr and Culture Methods.
Authors: Stachelska, M A
Journal: Polish journal of veterinary sciences (2017): 477-484
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20

VIC 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67002-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

VIC 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

VIC 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

VIC 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*    货号67002 货号 67002 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

VIC 染料 qPCR 校准板可用于维护您的 7500 实时 PCR 系统和快速 96 孔板。 对于大多数 qPCR 仪器,必要的校准应至少每六个月进行一次。 该校准板可以立即使用,无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。 

 

参考文献

Rapid screening method of Saccharomyces cerevisiae mutants using calcofluor white and aniline blue.
Authors: Perrine-Walker, Francine and Payne, Jennifer
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2021): 1077-1086
The P2X7 receptor antagonist JNJ-47965567 administered thrice weekly from disease onset does not alter progression of amyotrophic lateral sclerosis in SOD1G93A mice.
Authors: Ly, Diane and Dongol, Anjila and Cuthbertson, Peter and Guy, Thomas V and Geraghty, Nicholas J and Sophocleous, Reece A and Sin, Lucia and Turner, Bradley J and Watson, Debbie and Yerbury, Justin J and Sluyter, Ronald
Journal: Purinergic signalling (2020): 109-122
Development of the MitoQ assay as a real-time quantification of mitochondrial DNA in degraded samples.
Authors: Wai, Ka Tak and Gunn, Peter and Barash, Mark
Journal: International journal of legal medicine (2019): 411-417
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Lineage-specific detection of influenza B virus using real-time polymerase chain reaction with melting curve analysis.
Authors: Tewawong, Nipaporn and Chansaenroj, Jira and Klinfueng, Sirapa and Vichiwattana, Preeyaporn and Korkong, Sumeth and Thongmee, Thanunrat and Theamboonlers, Apiradee and Payungporn, Sunchai and Vongpunsawad, Sompong and Poovorawan, Yong
Journal: Archives of virology (2016): 1425-35
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
The effects of escitalopram on myocardial apoptosis and the expression of Bax and Bcl-2 during myocardial ischemia/reperfusion in a model of rats with depression.
Authors: Wang, Yiming and Zhang, Hongming and Chai, Fangxian and Liu, Xingde and Berk, Michael
Journal: BMC psychiatry (2014): 349
Transcriptional regulators Myb and BCL11A interplay with DNA methyltransferase 1 in developmental silencing of embryonic and fetal β-like globin genes.
Authors: Roosjen, Mark and McColl, Bradley and Kao, Betty and Gearing, Linden J and Blewitt, Marnie E and Vadolas, Jim
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014): 1610-20
Identification of an isogenic semidwarf rice cultivar carrying the Green Revolution sd1 gene by multiplex codominant ASP-PCR and SSR markers.
Authors: Naito, Yoshiki and Tomita, Motonori
Journal: Biochemical genetics (2013): 530-42

ROX 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67004-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ROX 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

ROX 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

ROX 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*    货号67004 货号 67004 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

ROX 染料 qPCR 校准板可用于维护配备 Fast 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器,必要的校准应至少每六个月进行一次。 该校准板可以立即使用,无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。 

 

参考文献

SARS-CoV-2 Detection using Real Time PCR by a Commercial Diagnostic Kit.
Authors: Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A
Journal: Mymensingh medical journal : MMJ (2020): 596-600
RT-qPCR Detection of Low-Copy HIV RNA with Yin-Yang Probes.
Authors: Kireev, Dmitry E and Farzan, Valentina M and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 27-35
The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027
Developmental validation of the Microreader™ 20A ID system.
Authors: Qu, Shengqiu and Li, Hang and Li, Yifan and Lv, Meili and Yang, Fan and Zhu, Jing and Yu, Zailiang and Liu, Yuqing and Chen, Chuguang and Wang, Yinji and Li, Zhuo and Zhang, Lin and Liang, Weibo
Journal: Electrophoresis (2019): 3099-3107
Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.
Authors: Aviv, Gili and Gal-Mor, Ohad
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 23-32
Detection of simultaneous multi-mutations using base-quenched probe.
Authors: Mao, Huihui and Luo, Guanghua and Zhang, Jun and Xu, Ning
Journal: Analytical biochemistry (2018): 79-81
Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Sun, Y and Qiao, B and Si, C C and Hu, P and Lu, S Y and Ren, H L and Liu, Z S and Qiu, H J and Liu, J Q
Journal: Biosensors & bioelectronics (2016): 194-199
Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701
Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67006-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*    货号67006 货号 67006 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

FAM 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器,必要的校准应至少每六个月进行一次。 该校准板可以立即使用,无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

Novel design of nucleic acid standards for hydrolysis probe-based PCR with melting analysis.
Authors: Baoutina, Anna and Bhat, Somanath
Journal: Gene therapy (2021)
Determination of Advantages and Limitations of qPCR Duplexing in a Single Fluorescent Channel.
Authors: Zhang, Haoqing and Yan, Zhiqiang and Wang, Xinlu and Gaňová, Martina and Chang, Honglong and Laššáková, Soňa and Korabecna, Marie and Neuzil, Pavel
Journal: ACS omega (2021): 22292-22300
SARS-CoV-2 Detection using Real Time PCR by a Commercial Diagnostic Kit.
Authors: Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A
Journal: Mymensingh medical journal : MMJ (2020): 596-600
Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity.
Authors: Menendez-Gil, Pilar and Caballero, Carlos J and Solano, Cristina and Toledo-Arana, Alejandro
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 41-58
Development and Evaluation of a Single Dye Duplex Droplet Digital PCR Assay for the Rapid Detection and Quantification of Mycobacterium tuberculosis.
Authors: Nyaruaba, Raphael and Xiong, Jin and Mwaliko, Caroline and Wang, Nuo and Kibii, Belindah J and Yu, Junping and Wei, Hongping
Journal: Microorganisms (2020)
A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor.
Authors: Shi, Bing and Li, Yuanming and Wu, Di and Wu, Wenming
Journal: The Analyst (2020): 2767-2773
Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions.
Authors: Tomé, Stéphanie and Gourdon, Geneviève
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 11-23
Ratiometric fluorescence sensor based on carbon dots as internal reference signal and T7 exonuclease-assisted signal amplification strategy for microRNA-21 detection.
Authors: Wang, Zhenzhen and Xue, Zhiqiang and Hao, Xiaoli and Miao, Chenfang and Zhang, Jianzhong and Zheng, Yanjie and Zheng, Zongfu and Lin, Xinhua and Weng, Shaohuang
Journal: Analytica chimica acta (2020): 212-219
PharmFrag: An Easy and Fast Multiplex Pharmacogenetics Assay to Simultaneously Analyze 9 Genetic Polymorphisms Involved in Response Variability of Anticancer Drugs.
Authors: Bouvet, Régis and Verdier, Marie-Clémence and El Baroudi, Yahya and Galibert, Marie-Dominique and David, Véronique and Schutz, Sacha and Tron, Camille
Journal: International journal of molecular sciences (2020)
The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67008-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*    货号67008 货号 67008 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

SYBR 染料 qPCR 校准板可用于维护配备 Fast 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器,必要的校准应至少每六个月进行一次。 该校准板可以立即使用,无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

Combined evaluation of proliferation and apoptosis to calculate IC50 of VPA-induced PANC-1 cells and assessing its effect on the Wnt signaling pathway.
Authors: Ekici, Yeliz and Yilmaz, Abdullah and Kucuksezer, Umut Can and Gazioglu, Sema Bilgic and Yamalioglu, Zeynep Dogusan and Gurol, Ali Osman and Linn, Thomas and Tuncer, Feyza Nur
Journal: Medical oncology (Northwood, London, England) (2021): 109
Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays.
Authors: Bonelli, Piero and Dei Giudici, Silvia and Peruzzu, Angela and Mura, Lorena and Santucciu, Cinzia and Maestrale, Caterina and Masala, Giovanna
Journal: Pathogens (Basel, Switzerland) (2021)
Expression levels of serum circulating microRNAs in pediatric patients with ventricular and supraventricular arrhythmias.
Authors: Moric-Janiszewska, Ewa and Smolik, Sławomir and Morka, Aleksandra and Szydłowski, Lesław and Kapral, Małgorzata
Journal: Advances in medical sciences (2021): 411-417
RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors.
Authors: Munawar, Mustafa Ahmad and Martin, Frank and Toljamo, Anna and Kokko, Harri and Oksanen, Elina
Journal: BioTechniques (2020): 270-280
Rapid detection of porcine DNA in processed food samples using a streamlined DNA extraction method combined with the SYBR Green real-time PCR assay.
Authors: Tan, Lee Lee and Ahmed, Siti Aminah and Ng, Siew Kit and Citartan, Marimuthu and Raabe, Carsten A and Rozhdestvensky, Timofey S and Tang, Thean Hock
Journal: Food chemistry (2020): 125654
Expression of SARS-CoV-2 receptor ACE2 and TMPRSS2 in human primary conjunctival and pterygium cell lines and in mouse cornea.
Authors: Ma, Di and Chen, Chong-Bo and Jhanji, Vishal and Xu, Ciyan and Yuan, Xiang-Ling and Liang, Jia-Jian and Huang, Yuqiang and Cen, Ling-Ping and Ng, Tsz Kin
Journal: Eye (London, England) (2020): 1212-1219
Preoperative heat shock protein 47 levels identify colorectal cancer patients with lymph node metastasis and poor prognosis.
Authors: Mori, Koichiro and Toiyama, Yuji and Okugawa, Yoshinaga and Ichikawa, Takashi and Nagano, Yuka and Oki, Satoshi and Shimura, Tadanobu and Fujikawa, Hiroyuki and Hiro, Junichiro and Kobayash, Minako and Araki, Toshimitsu and Inoue, Yasuhiro and Mohri, Yasuhiko and Kusunoki, Masato
Journal: Oncology letters (2020): 333
Distribution of virulence genes in bacteremic methicillin-resistant Staphylococcus aureus isolates from various sources.
Authors: Wang, Fu-Der and Wu, Ping-Feng and Chen, Su-Jung
Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (2019): 426-432
Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance.
Authors: Saeed, Noor AlHuda Ali A H and Hamzah, Israa Hussein and Al-Gharrawi, Samar Abdul Raheem
Journal: BMC research notes (2019): 455
Aberrant Expression of the miR-181b/miR-222 after Hematopoietic Stem Cell Transplantation in Patients with Acute Myeloid Leukemia.
Authors: Iravani Saadi, Mahdiyar and Arandi, Nargess and Yaghobi, Ramin and Azarpira, Negar and Geramizadeh, Bita and Ramzi, Mani
Journal: Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion (2019): 446-450

NED 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67010-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

NED 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

NED 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

NED 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*    货号67010 货号 67010 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

NED 染料 qPCR 校准板可用于维护您的 7500 实时 PCR 系统和快速 96 孔板。 对于大多数 qPCR 仪器,必要的校准应至少每六个月进行一次。 该校准板可以立即使用,无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。 

 

参考文献

DNA degradation in human teeth exposed to thermal stress.
Authors: Lozano-Peral, Diego and Rubio, Leticia and Santos, Ignacio and Gaitán, María Jesús and Viguera, Enrique and Martín-de-Las-Heras, Stella
Journal: Scientific reports (2021): 12118
Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
An improved rapid quantitative detection and identification method for a wide range of fungi.
Authors: Soeta, Nobutoshi and Terashima, Masanori and Gotoh, Mitsukazu and Mori, Shuichi and Nishiyama, Kyoko and Ishioka, Ken and Kaneko, Hisatoshi and Suzutani, Tatsuo
Journal: Journal of medical microbiology (2009): 1037-1044
Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK.
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36
TaqMan reverse transcriptase-polymerase chain reaction coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type.
Authors: Luthra, Rajyalakshmi and Medeiros, L Jeffrey
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 135-45
TaqMan RT-PCR assay coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type.
Authors: Luthra, Rajyalakshmi and Sanchez-Vega, Beatriz and Medeiros, L Jeffrey
Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (2004): 96-103
Development of a 17-plex microsatellite polymerase chain reaction kit for genotyping horses.
Authors: Dimsoski, Pero
Journal: Croatian medical journal (2003): 332-5
Semiquantitative and qualitative assessment of B-lymphocyte V H repertoire by a fluorescent multiplex PCR.
Authors: Feuchtenberger, Martin and Tony, Hans-Peter and Rouzière, Anne-Sophie and Jacobi, Anette and Dörner, Thomas and Kneitz, Christian and Starostik, Petr
Journal: Journal of immunological methods (2003): 121-7

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67012-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*    货号67012 货号 67012 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

JOE 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器,必要的校准应至少每六个月进行一次。 该校准板可以立即使用,无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027
Detection of simultaneous multi-mutations using base-quenched probe.
Authors: Mao, Huihui and Luo, Guanghua and Zhang, Jun and Xu, Ning
Journal: Analytical biochemistry (2018): 79-81
Molecular beacons with JOE dye: Influence of linker and 3′ couple quencher.
Authors: Tsybulsky, Dmitry A and Kvach, Maksim V and Ryazantsev, Dmitry Y and Aparin, Ilya O and Stakheev, Alexander A and Prokhorenko, Igor A and Martynenko, Yury V and Gontarev, Sergey V and Formanovsky, Andrey A and Zatsepin, Timofei S and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Molecular and cellular probes (2016): 285-290
Mineralization Effect of Hyaluronan on Dental Pulp Cells via CD44.
Authors: Chen, Kuan-Liang and Yeh, Ying-Yi and Lung, Jrhau and Yang, Yu-Chi and Yuan, Kuo
Journal: Journal of endodontics (2016): 711-6
Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay.
Authors: Maksyutov, Rinat A and Gavrilova, Elena V and Shchelkunov, Sergei N
Journal: Journal of virological methods (2016): 215-220
Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes.
Authors: Skoblov, Aleksander Yu and Vichuzhanin, Maxim V and Farzan, Valentina M and Veselova, Olga A and Konovalova, Tatiana A and Podkolzin, Alexander T and Shipulin, German A and Zatsepin, Timofei S
Journal: Bioorganic & medicinal chemistry (2015): 6749-56
Combined effects of simvastatin and enamel matrix derivative on odontoblastic differentiation of human dental pulp cells.
Authors: Karanxha, Lorena and Park, Su-Jung and Son, Won-Jun and Nör, Jacques E and Min, Kyung-San
Journal: Journal of endodontics (2013): 76-82
In vivo quantitative evaluation of live and dead bacteria in root canal infection by using propidium monoazide with real-time PCR.
Authors: Kim, Sin-Young and Shin, Yooseok and Lee, Chan-Young and Jung, Il-Young
Journal: Journal of endodontics (2013): 1359-63
Two-dye and one- or two-quencher DNA probes for real-time PCR assay: synthesis and comparison with a TaqMan™ probe.
Authors: Ryazantsev, Dmitry Y and Tsybulsky, Dmitry A and Prokhorenko, Igor A and Kvach, Maksim V and Martynenko, Yury V and Philipchenko, Pavel M and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Analytical and bioanalytical chemistry (2012): 59-68
ADAM28 manipulates proliferation, differentiation, and apoptosis of human dental pulp stem cells.
Authors: Zhao, Zheng and Liu, Hongchen and Wang, Dongsheng
Journal: Journal of endodontics (2011): 332-9

7 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67020-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

7 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

7 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

7 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*    货号67020 货号 67020 存储条件 在零下15度以下保存, 避免光照
规格 1 Set 价格 11400
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

7 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器,必要的校准应至少每六个月进行一次。 该校准板可以立即使用,无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

Evaluation of the Thermo Scientific SureTect Salmonella Species PCR Assay in a Broad Range of Foods and Select Environmental Surfaces: Pre-Collaborative and Collaborative Study: First Action 2021.02.
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Leonte, Ana-Maria and Vandoros, Evangelos J and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022): 167-190
Development and Evaluation of a TaqMan Real-Time PCR Assay for the Rapid Detection of Cross-Contamination of RD (Human) and L20B (Mouse) Cell Lines Used in Poliovirus Surveillance.
Authors: Ahmad, Ausaf and Lee, Joo R and Metz, John M and Tang, Xiaoling and Lin, Seh-Ching and Bagarozzi, Dennis A and Petway, David and Herzegh, Owen
Journal: Journal of virological methods (2022): 114354
Evaluation of the Thermo ScientificTM SureTectTMListeria monocytogenes PCR Assay in a Broad Range of Foods and Selected Environmental Surfaces: Pre-Collaborative and Collaborative Study, First Action 2021.05.
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Vandoros, Evangelos J and Leonte, Ana-Maria and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022)
The Comparison of Real-time-PCR-HRM and Microscopy Methods for Detection of Mixed Plasmodium spp. Infections in Laghman Province, Afghanistan.
Authors: Dalimi, Abdolhossein and Mosawi, Sayed Hussain
Journal: Infectious disorders drug targets (2021): 399-404
Clinical evaluation of a multiplex real-time RT-PCR assay for detection of SARS-CoV-2 in individual and pooled upper respiratory tract samples.
Authors: Laverack, Melissa and Tallmadge, Rebecca L and Venugopalan, Roopa and Cronk, Brittany and Zhang, XiuLin and Rauh, Rolf and Saunders, Amy and Nelson, William M and Plocharczyk, Elizabeth and Diel, Diego G
Journal: Archives of virology (2021): 2551-2561
TaqMan real time PCR for the Detection of the Gilbert’s Syndrome Markers UGT1A1*28; UGT1A1*36 and UGT1A1*37.
Authors: Daprà, Valentina and Alliaudi, Carla and Galliano, Ilaria and Dini, Maddalena and Curcio, Giada Lo and Calvi, Cristina and Archetti, Marialaura and Gavatorta, Martina and Bergallo, Massimiliano
Journal: Molecular biology reports (2021): 4953-4959
Rapid and Safe Detection of SARS-CoV-2 and Influenza Virus RNA Using Onsite Quantitative PCR Diagnostic Testing From Clinical Specimens Collected in Molecular Transport Medium.
Authors: Daum, Luke T and Fischer, Gerald W
Journal: The journal of applied laboratory medicine (2021): 1409-1416
Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay.
Authors: Potter, Robert F and Abro, Brooj and Eby, Charles S and Burnham, Carey-Ann D and Anderson, Neil W and Parikh, Bijal A
Journal: Diagnostic microbiology and infectious disease (2021): 115387
Validation of the Thermo Scientific™ SureTect™ Staphylococcus aureus PCR Assay for the Detection of Staphylococcus aureus in Dairy Matrices: AOAC Performance Tested MethodsSM 052101.
Authors: Evans, Katharine and Faulds, Nikki and Crabtree, David and Hughes, Annette and Sohier, Daniele and Manthe, Craig and Hahs, Matthew and Heikkinen, Pauliina and Hurskainen, Emmi and Koch, Kateland and Thompson, Wesley and Bastin, Benjamin and Benzinger, M Joseph
Journal: Journal of AOAC International (2021)
Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2.
Authors: Moore, Nicholas M and Li, Haiying and Schejbal, Debra and Lindsley, Jennifer and Hayden, Mary K
Journal: Journal of clinical microbiology (2020)

Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67014-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*    货号67014 货号 67014 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:67014

产品名称:Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

规格:1 Plate

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

外观:液体

溶剂:DMSO

 

产品介绍

Cy3.5 Dye qPCR 校准板可用于维护您的 7500 Real-Time PCR 系统和快速 96 孔模块。对于大多数 qPCR 仪器,必须至少每六个月校准一次设备。该校准板无需任何额外的准备步骤即可使用。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来显着改善多路复用的 qPCR 结果。有关详细的校准操作,请参阅您的仪器指南。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Cy3.5 染料 qPCR 校准板。

  

参考文献

Development of a rapid qPCR method to quantify lactic acid bacteria in cold-smoked salmon.
Authors: Jérôme, Marc and Passerini, Delphine and Chevalier, Frédérique and Marchand, Laetitia and Leroi, Françoise and Macé, Sabrina
Journal: International journal of food microbiology (2022): 109504
Semi-Quantitative Detection of Drosophila suzukii (Diptera: Drosophilidae) From Bulk Trap Samples Using PCR Technology.
Authors: Renkema, Justin M and McFadden-Smith, Wendy and Chen, Shu
Journal: Journal of economic entomology (2022)
Continuous polymerase chain reaction microfluidics integrated with a gold-capped nanoslit sensing chip for Epstein-Barr virus detection.
Authors: Hsieh, Han-Yun and Chang, Ray and Huang, Yung-Yu and Juan, Po-Han and Tahara, Hidetoshi and Lee, Kuan-Yi and Vo, Di Ngoc Kha and Tsai, Ming-Han and Wei, Pei-Kuen and Sheen, Horn-Jiunn and Fan, Yu-Jui
Journal: Biosensors & bioelectronics (2022): 113672
XCL1 Aggravates Diabetic Nephropathy-Mediated Renal Glomerular Endothelial Cell Apoptosis and Inflammatory Response via Regulating p53/Nuclear Factor-Kappa B Pathway.
Authors: Zhang, Yuan and Chen, Xiaolan and Fan, Yaping and Liu, Jing and Yuan, Li
Journal: Nephron (2022): 84-98
DNA-based quantification of Fusarium oxysporum f. sp. vasinfectum in environmental soils to describe spatial variation in inoculum density.
Authors: Davis, Roy and Isakeit, Thomas and Chappell, Thomas
Journal: Plant disease (2022)
First case of SARS-CoV-2 RNA detection in municipal solid waste leachate from Brazil.
Authors: Mondelli, Giulliana and Silva, Ednei Rodrigues and Claro, Ieda Carolina Mantovani and Augusto, Matheus Ribeiro and Duran, Adriana Feliciano Alves and Cabral, Aline Diniz and de Moraes Bomediano Camillo, Lívia and Dos Santos Oliveira, Luísa Helena and de Freitas Bueno, Rodrigo
Journal: The Science of the total environment (2022): 153927
Precise RNA Quantification by Counting Individual RNA Molecules Using High-Sensitivity Capillary Flow Cytometry.
Authors: Yoo, Hee-Bong and Park, Sang-Ryoul and Hong, Kee-Suk and Yang, Inchul
Journal: Analytical chemistry (2022): 1752-1759
Accurate qPCR quantification in polymicrobial communities requires assessment of gDNA extraction efficiency.
Authors: Cerca, Nuno and Lima, Ângela and França, Angela
Journal: Journal of microbiological methods (2022): 106421
Lentiviral standards to determine the sensitivity of assays that quantify lentiviral vector copy numbers and genomic insertion sites in cells.
Authors: Corre, Guillaume and Seye, Ababacar and Frin, Sophie and Ferrand, Maxime and Winkler, Kathrin and Luc, Cyril and Dorange, Fabien and Rocca, Céline J and Galy, Anne
Journal: Gene therapy (2022)
One-Step Multiplexed Droplet Digital Polymerase Chain Reaction for Quantification of p190 BCR-ABL1 Fusion Transcript in B-Lymphoblastic Leukemia.
Authors: Martinez, Ryan J and Kang, Qing and Nennig, Davis and Bailey, Nathanael G and Brown, Noah A and Betz, Bryan L and Tewari, Muneesh and Thyagarajan, Bharat and Bachanova, Veronika and Mroz, Pawel
Journal: Archives of pathology & laboratory medicine (2022): 92-100

TAQuest qPCR Master Mix with Helixyte Green *低ROX* 货号17272-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix with Helixyte Green *低ROX*

TAQuest qPCR Master Mix with Helixyte Green *低ROX*

TAQuest qPCR Master Mix with Helixyte Green *低ROX*    货号17272 货号 17272 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17272

产品名称:TAQuest qPCR Master Mix with Helixyte Green *低ROX*

规格:1ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液,针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可运行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用,可确保对所有样品类型(如基因组、质粒、病毒和 cDNA 模板)的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析DNA。该预混液包含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *低ROX*。 

适用仪器


qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *低ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *低 ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus.
Authors: Wang, Yong and Sun, Jianfei and Guo, Xu and Li, Wei and Zhang, Da and Liu, Guangqing and Zhou, Tianhong and Li, Yongdong
Journal: Molecular and cellular probes (2021): 101762

A duplex SYBR green I-based real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus.
Authors: Sun, Liting and Xu, Zhiqing and Wu, Junhuang and Cui, Yongqiu and Guo, Xu and Xu, Fazhi and Li, Yongdong and Wang, Yong
Journal: Journal of virological methods (2021): 114294

A new SYBR Green real-time PCR to detect SARS-CoV-2.
Authors: Marinowic, D R and Zanirati, G and Rodrigues, F V F and Grahl, M V C and Alcará, A M and Machado, D C and Da Costa, J C
Journal: Scientific reports (2021): 2224

A novel duplex SYBR Green real-time PCR with melting curve analysis method for beef adulteration detection.
Authors: Li, Jiapeng and Wei, Yixuan and Li, Jinchun and Liu, Ruixi and Xu, Suigen and Xiong, Suyue and Guo, Ya and Qiao, Xiaoling and Wang, Shouwei
Journal: Food chemistry (2021): 127932

A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR.
Authors: Abdel Sater, Fadil and Younes, Mahmoud and Nassar, Hassan and Nguewa, Paul and Hamze, Kassem
Journal: Molecular biology reports (2021): 7243-7249

Design and characterization of a SYBR Green I-based melting curve method for investigation of HER2I655V polymorphism in breast cancer.
Authors: Desriani and Azamris and Ghaissani, Shabrina S and Kinanti, Senja R and Warisman, Muhammad A and Fitria, N
Journal: Journal, genetic engineering & biotechnology (2021): 6

Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards.
Authors: Olveira, José G and Souto, Sandra and Bandín, Isabel and Dopazo, Carlos P
Journal: Animals : an open access journal from MDPI (2021)

Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of swine Getah virus.
Authors: Xia, Yin-He and Shi, Zi-Cong and Wang, Xin-Wei and Li, Yong-Tao and Wang, Zeng and Chang, Hong-Tao and Liu, Hong-Ying and Chen, Lu and Wang, Chuan-Qing and Yang, Xia
Journal: Molecular and cellular probes (2021): 101730

Development of New PCR Assay with SYBR Green I for Detection of Mycoplasma, Acholeplasma, and Ureaplasma sp. in Cell Cultures.
Authors: Krzysztoń-Russjan, Jolanta and Chudziak, Jakub and Bednarek, Małgorzata and Anuszewska, Elżbieta Lidia
Journal: Diagnostics (Basel, Switzerland) (2021)

Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection.
Authors: Wang, Yong and Li, Wei and Guo, Xu and Zhang, Da and Sun, Jianfei and Fu, Ziteng and Liu, Guangqing and Li, Yongdong and Jiang, Shudong
Journal: 3 Biotech (2021): 61

TAQuest qPCR Master Mix with Helixyte Green *低ROX* 货号17273-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix with Helixyte Green *低ROX*

TAQuest qPCR Master Mix with Helixyte Green *低ROX*

TAQuest qPCR Master Mix with Helixyte Green *低ROX*    货号17273 货号 17273 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17273

产品名称:TAQuest qPCR Master Mix with Helixyte Green *低ROX*

规格:5ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液,针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可运行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用,可确保对所有样品类型(如基因组、质粒、病毒和 cDNA 模板)的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析DNA。该预混液包含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *低ROX*。 

 

适用仪器


qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *低ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *低 ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus.
Authors: Wang, Yong and Sun, Jianfei and Guo, Xu and Li, Wei and Zhang, Da and Liu, Guangqing and Zhou, Tianhong and Li, Yongdong
Journal: Molecular and cellular probes (2021): 101762

A duplex SYBR green I-based real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus.
Authors: Sun, Liting and Xu, Zhiqing and Wu, Junhuang and Cui, Yongqiu and Guo, Xu and Xu, Fazhi and Li, Yongdong and Wang, Yong
Journal: Journal of virological methods (2021): 114294

A new SYBR Green real-time PCR to detect SARS-CoV-2.
Authors: Marinowic, D R and Zanirati, G and Rodrigues, F V F and Grahl, M V C and Alcará, A M and Machado, D C and Da Costa, J C
Journal: Scientific reports (2021): 2224

A novel duplex SYBR Green real-time PCR with melting curve analysis method for beef adulteration detection.
Authors: Li, Jiapeng and Wei, Yixuan and Li, Jinchun and Liu, Ruixi and Xu, Suigen and Xiong, Suyue and Guo, Ya and Qiao, Xiaoling and Wang, Shouwei
Journal: Food chemistry (2021): 127932

A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR.
Authors: Abdel Sater, Fadil and Younes, Mahmoud and Nassar, Hassan and Nguewa, Paul and Hamze, Kassem
Journal: Molecular biology reports (2021): 7243-7249

Design and characterization of a SYBR Green I-based melting curve method for investigation of HER2I655V polymorphism in breast cancer.
Authors: Desriani and Azamris and Ghaissani, Shabrina S and Kinanti, Senja R and Warisman, Muhammad A and Fitria, N
Journal: Journal, genetic engineering & biotechnology (2021): 6

Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards.
Authors: Olveira, José G and Souto, Sandra and Bandín, Isabel and Dopazo, Carlos P
Journal: Animals : an open access journal from MDPI (2021)

Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of swine Getah virus.
Authors: Xia, Yin-He and Shi, Zi-Cong and Wang, Xin-Wei and Li, Yong-Tao and Wang, Zeng and Chang, Hong-Tao and Liu, Hong-Ying and Chen, Lu and Wang, Chuan-Qing and Yang, Xia
Journal: Molecular and cellular probes (2021): 101730

Development of New PCR Assay with SYBR Green I for Detection of Mycoplasma, Acholeplasma, and Ureaplasma sp. in Cell Cultures.
Authors: Krzysztoń-Russjan, Jolanta and Chudziak, Jakub and Bednarek, Małgorzata and Anuszewska, Elżbieta Lidia
Journal: Diagnostics (Basel, Switzerland) (2021)

Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection.
Authors: Wang, Yong and Li, Wei and Guo, Xu and Zhang, Da and Sun, Jianfei and Fu, Ziteng and Liu, Guangqing and Li, Yongdong and Jiang, Shudong
Journal: 3 Biotech (2021): 61

TAQuest qPCR Master Mix with Helixyte Green *高ROX* 货号17274-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix with Helixyte Green *高ROX*

TAQuest qPCR Master Mix with Helixyte Green *高ROX*

货号 17274 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17274

产品名称:TAQuest qPCR Master Mix with Helixyte Green *高ROX*

规格:1ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液,针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可运行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用,可确保对所有样品类型(如基因组、质粒、病毒和 cDNA 模板)的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析DNA。该预混液包含大量ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *高ROX*。 

 

适用仪器


qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *高ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *高 ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Aligned Expression of IFI16 and STING Genes in RRMS Patients’ Blood.
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886

SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30

Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.
Authors: Aviv, Gili and Gal-Mor, Ohad
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 23-32

An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Sun, Y and Qiao, B and Si, C C and Hu, P and Lu, S Y and Ren, H L and Liu, Z S and Qiu, H J and Liu, J Q
Journal: Biosensors & bioelectronics (2016): 194-199

Analysis of P. gingivalis, T. forsythia and S. aureus levels in edentulous mouths prior to and 6 months after placement of one-piece zirconia and titanium implants.
Authors: Siddiqi, Allauddin and Milne, Trudy and Cullinan, Mary P and Seymour, Gregory J
Journal: Clinical oral implants research (2016): 288-94

Real-time immuno-PCR for ultrasensitive detection of pyrene and other homologous PAHs.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Zhang, Y Y and Qiao, B and Sun, Y and Yang, L and Hu, P and Lu, S Y and Ren, H L and Zhang, J H and Wang, X R and Liu, Z S
Journal: Biosensors & bioelectronics (2015): 42-7

Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis.
Authors: Ramos, Carlos A N and Araújo, Flábio R and Souza, Ingrid I F and Bacanelli, G and Luiz, Hera L and Russi, Lívia S and Oliveira, Renato H M and Soares, Cleber O and Rosinha, Grácia M S and Alves, Leucio C
Journal: Veterinary parasitology (2011): 79-83

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX* 货号17276-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*    货号17276 货号 17276 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17276

产品名称:TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

规格:1ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

TAQuest FAST qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液,针对 qPCR 和两步法 RT-qPCR 进行了优化。对于 20 uL 反应体积中的 40 个 PCR 循环,预混液可在 50 分钟内提供结果。该混合物包括含有 dNTP 的优化缓冲液和我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶,该酶旨在允许即时热启动,最大限度地减少非特异性产物的形成,从而允许室温反应设置。运行所需的 PCR 反应只需要模板和目标引物。 TAQuest FAST qPCR Master Mix 与 Helixyte Green 确保 PCR 特异性和灵敏度对所有样品类型,如基因组、质粒、病毒和 cDNA 模板。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析 DNA。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*。 

 

适用仪器


qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *无ROX* 解冻 TAQuest™ FAST qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *无 ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Fatal systemic toxoplasmosis in a 3-month-old young tibetan goat (Capra hircus).
Authors: Pavone, Silvia and Crotti, Silvia and Cruciani, Deborah and D’Avino, Nicoletta and Zema, Jacopo and Morelli, Simone and Gobbi, Marco and Madeo, Laura
Journal: BMC veterinary research (2020): 423

Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus).
Authors: Kang, Tae Sun
Journal: Food chemistry (2019): 1-8

A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants.
Authors: Sebastiani, Carla and Curcio, Ludovica and Ciullo, Marcella and Cruciani, Deborah and Crotti, Silvia and Pesca, Cristina and Torricelli, Martina and Sebastianelli, Martina and Felici, Andrea and Biagetti, Massimo
Journal: Journal of microbiological methods (2018): 12-17

A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species.
Authors: Kim, Mi-Ra and Kwon, Kisung and Jung, Yoo-Kyung and Kang, Tae Sun
Journal: Food chemistry (2018): 112-119

Development of a Sensitive Real-Time Fast-qPCR Based on SYBR® Green for Detection and Quantification of Chicken Parvovirus (ChPV).
Authors: Nuñez, Luis F and Santander-Parra, Silvana H and Chaible, Lucas and De la Torre, David I and Buim, Marcos R and Murakami, Alexandre and Zaidan Dagli, Maria Lucia and Astolfi-Ferreira, Claudete S and Piantino Ferreira, Antonio J
Journal: Veterinary sciences (2018)

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Fast quantitative PCR, locked nucleic acid probes and reduced volume reactions are effective tools for detecting Batrachochytrium dendrobatidis DNA.
Authors: Ruthig, Gregory R and Deridder, Benjamin P
Journal: Diseases of aquatic organisms (2012): 249-53

Multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness.
Authors: Buzard, Gregory S and Baker, Daniel and Wolcott, Mark J and Norwood, David A and Dauphin, Leslie A
Journal: Forensic science international (2012): 292-7

Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles.
Authors: Yoder, Kristine E and Fishel, Richard
Journal: Journal of virological methods (2008): 253-6

Detection of equine herpesvirus-1 in nasal swabs of horses by quantitative real-time PCR.
Authors: Perkins, G A and Goodman, L B and Dubovi, E J and Kim, S G and Osterrieder, N
Journal: Journal of veterinary internal medicine: 1234-8

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX* 货号17277-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*    货号17277 货号 17277 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17277

产品名称:TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

规格:5ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

TAQuest FAST qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液,针对 qPCR 和两步法 RT-qPCR 进行了优化。对于 20 uL 反应体积中的 40 个 PCR 循环,预混液可在 50 分钟内提供结果。该混合物包括含有 dNTP 的优化缓冲液和我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶,该酶旨在允许即时热启动,最大限度地减少非特异性产物的形成,从而允许室温反应设置。运行所需的 PCR 反应只需要模板和目标引物。 TAQuest FAST qPCR Master Mix 与 Helixyte Green 确保 PCR 特异性和灵敏度对所有样品类型,如基因组、质粒、病毒和 cDNA 模板。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析 DNA。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*。 

 

适用仪器


qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *无ROX* 解冻 TAQuest™ FAST qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *无 ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Fatal systemic toxoplasmosis in a 3-month-old young tibetan goat (Capra hircus).
Authors: Pavone, Silvia and Crotti, Silvia and Cruciani, Deborah and D’Avino, Nicoletta and Zema, Jacopo and Morelli, Simone and Gobbi, Marco and Madeo, Laura
Journal: BMC veterinary research (2020): 423

Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus).
Authors: Kang, Tae Sun
Journal: Food chemistry (2019): 1-8

A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants.
Authors: Sebastiani, Carla and Curcio, Ludovica and Ciullo, Marcella and Cruciani, Deborah and Crotti, Silvia and Pesca, Cristina and Torricelli, Martina and Sebastianelli, Martina and Felici, Andrea and Biagetti, Massimo
Journal: Journal of microbiological methods (2018): 12-17

A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species.
Authors: Kim, Mi-Ra and Kwon, Kisung and Jung, Yoo-Kyung and Kang, Tae Sun
Journal: Food chemistry (2018): 112-119

Development of a Sensitive Real-Time Fast-qPCR Based on SYBR® Green for Detection and Quantification of Chicken Parvovirus (ChPV).
Authors: Nuñez, Luis F and Santander-Parra, Silvana H and Chaible, Lucas and De la Torre, David I and Buim, Marcos R and Murakami, Alexandre and Zaidan Dagli, Maria Lucia and Astolfi-Ferreira, Claudete S and Piantino Ferreira, Antonio J
Journal: Veterinary sciences (2018)

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Fast quantitative PCR, locked nucleic acid probes and reduced volume reactions are effective tools for detecting Batrachochytrium dendrobatidis DNA.
Authors: Ruthig, Gregory R and Deridder, Benjamin P
Journal: Diseases of aquatic organisms (2012): 249-53

Multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness.
Authors: Buzard, Gregory S and Baker, Daniel and Wolcott, Mark J and Norwood, David A and Dauphin, Leslie A
Journal: Forensic science international (2012): 292-7

Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles.
Authors: Yoder, Kristine E and Fishel, Richard
Journal: Journal of virological methods (2008): 253-6

Detection of equine herpesvirus-1 in nasal swabs of horses by quantitative real-time PCR.
Authors: Perkins, G A and Goodman, L B and Dubovi, E J and Kim, S G and Osterrieder, N
Journal: Journal of veterinary internal medicine: 1234-8

TAQuest qPCR Master Mix 用于TaqMan探针*高ROX* 货号17286-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

货号 17286 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17286

产品名称:TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

规格:1ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液,针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分,包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP,但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型(如基因组、质粒、病毒和 cDNA 模板)的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 

 

适用仪器


qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix 用于TaqMan探针*高ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701

Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9

[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6

[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61

[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX* 货号17289-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*    货号17289 货号 17289 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17289

产品名称:TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*

规格:5ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液,针对 qPCR 和两步法 RT-qPCR 进行了优化,非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容,因此在 20 uL 反应体积中进行 40 个 PCR 循环,可在 50 分钟内提供结果。预混液提供所有基本成分,包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP,但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型(如基因组、质粒、病毒和 cDNA 模板)的 PCR 特异性和灵敏度。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*。 

 

适用仪器


qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest  FAST qPCR Master Mix 用于TaqMan探针*无ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Evaluation of the efficiency of TaqMan duplex real-time PCR assay for non-invasive pre-natal assessment of foetal sex in equine.
Authors: Kadivar, Ali and Rashidzadeh, Habiballah and Davoodian, Najmeh and Nazari, Hasan and Dehghani Tafti, Rohallah and Heidari Khoei, Heidar and Seidi Samani, Hasan and Modaresi, Jahangir and Ahmadi, Ebrahim
Journal: Reproduction in domestic animals = Zuchthygiene (2021): 287-291

Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.
Authors: Chu, Son V and Vu, Son T and Nguyen, Hang M and Le, Ngan T and Truong, Phuong T and Vu, Van T T and Phung, Thuy T B and Nguyen, Anh T V
Journal: Journal of clinical microbiology (2021): e0093621

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae).
Authors: Aguirre, Carlos and Sánchez, Evelyn and Olivares, Natalia and Hinrichsen, Patricio
Journal: Journal of economic entomology (2021): 90-99

A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus.
Authors: Yang, Kan-Kan and Yin, Dong-Dong and Xu, Liang and Liang, Yue-Qiao and Tu, Jian and Song, Xiang-Jun and Shao, Ying and Liu, Hong-Mei and Qi, Ke-Zong
Journal: Molecular and cellular probes (2020): 101564

A development strategy to fast establish the Taqman qPCR based method to detect SNP mutations.
Authors: Jiang, Xiaohui and Xiang, Junbei and Wang, Ruifeng and Wan, Qian
Journal: Human cell (2020): 1331-1333

BlueTYPE – A low density TaqMan-RT-qPCR array for the identification of all 24 classical Bluetongue virus serotypes.
Authors: Ries, Christina and Beer, Martin and Hoffmann, Bernd
Journal: Journal of virological methods (2020): 113881

Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe.
Authors: Haddar, Cyrille and Verhoeven, Paul O and Bourlet, Thomas and Pozzetto, Bruno and Pillet, Sylvie
Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2020): 104636

Development of a new TaqMan-based PCR assay for the specific detection and quantification of Simkania negevensis.
Authors: Baccari, Olfa and Elleuch, Jihen and Barkallah, Mohamed and Boukedi, Hanen and Ayed, Nourelhouda Ben and Hammami, Adnene and Fendri, Imen and Abdelkafi, Slim
Journal: Molecular and cellular probes (2020): 101645

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.
Authors: Wang, Ruyi and Zhang, Wenyan and Ye, Rui and Pan, Zhongzhou and Li, Gairu and Su, Shuo
Journal: Molecular and cellular probes (2020): 101618

Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.
Authors: Nejati, Fatemeh and Junne, Stefan and Kurreck, Jens and Neubauer, Peter
Journal: Frontiers in microbiology (2020): 1291

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX* 货号17291-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*    货号17291 货号 17291 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17291

产品名称:TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

规格:5ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液,针对 qPCR 和两步法 RT-qPCR 进行了优化,非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容,因此在 20 uL 反应体积中进行 40 个 PCR 循环,可在 50 分钟内提供结果。预混液提供所有基本成分,包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP,但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型(如基因组、质粒、病毒和 cDNA 模板)的 PCR 特异性和灵敏度。该预混液含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*。

 

适用仪器


qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Evaluation of the efficiency of TaqMan duplex real-time PCR assay for non-invasive pre-natal assessment of foetal sex in equine.
Authors: Kadivar, Ali and Rashidzadeh, Habiballah and Davoodian, Najmeh and Nazari, Hasan and Dehghani Tafti, Rohallah and Heidari Khoei, Heidar and Seidi Samani, Hasan and Modaresi, Jahangir and Ahmadi, Ebrahim
Journal: Reproduction in domestic animals = Zuchthygiene (2021): 287-291

Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.
Authors: Chu, Son V and Vu, Son T and Nguyen, Hang M and Le, Ngan T and Truong, Phuong T and Vu, Van T T and Phung, Thuy T B and Nguyen, Anh T V
Journal: Journal of clinical microbiology (2021): e0093621

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae).
Authors: Aguirre, Carlos and Sánchez, Evelyn and Olivares, Natalia and Hinrichsen, Patricio
Journal: Journal of economic entomology (2021): 90-99

A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus.
Authors: Yang, Kan-Kan and Yin, Dong-Dong and Xu, Liang and Liang, Yue-Qiao and Tu, Jian and Song, Xiang-Jun and Shao, Ying and Liu, Hong-Mei and Qi, Ke-Zong
Journal: Molecular and cellular probes (2020): 101564

A development strategy to fast establish the Taqman qPCR based method to detect SNP mutations.
Authors: Jiang, Xiaohui and Xiang, Junbei and Wang, Ruifeng and Wan, Qian
Journal: Human cell (2020): 1331-1333

BlueTYPE – A low density TaqMan-RT-qPCR array for the identification of all 24 classical Bluetongue virus serotypes.
Authors: Ries, Christina and Beer, Martin and Hoffmann, Bernd
Journal: Journal of virological methods (2020): 113881

Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe.
Authors: Haddar, Cyrille and Verhoeven, Paul O and Bourlet, Thomas and Pozzetto, Bruno and Pillet, Sylvie
Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2020): 104636

Development of a new TaqMan-based PCR assay for the specific detection and quantification of Simkania negevensis.
Authors: Baccari, Olfa and Elleuch, Jihen and Barkallah, Mohamed and Boukedi, Hanen and Ayed, Nourelhouda Ben and Hammami, Adnene and Fendri, Imen and Abdelkafi, Slim
Journal: Molecular and cellular probes (2020): 101645

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.
Authors: Wang, Ruyi and Zhang, Wenyan and Ye, Rui and Pan, Zhongzhou and Li, Gairu and Su, Shuo
Journal: Molecular and cellular probes (2020): 101618

Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.
Authors: Nejati, Fatemeh and Junne, Stefan and Kurreck, Jens and Neubauer, Peter
Journal: Frontiers in microbiology (2020): 1291

TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX* 货号17292-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*

货号 17292 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17292

产品名称:TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*

规格:1ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液,针对 qPCR 和两步法 RT-qPCR 进行了优化,非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容,因此在 20 uL 反应体积中进行 40 个 PCR 循环,可在 50 分钟内提供结果。预混液提供所有基本成分,包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP,但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型(如基因组、质粒、病毒和 cDNA 模板)的 PCR 特异性和灵敏度。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*。

 

适用仪器


qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701

Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9

[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6

[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61

[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70