多孔型YMC-BioPro QA系列离子交换色谱柱

多孔型YMC-BioPro QA系列离子交换色谱柱

多孔型YMC-BioPro QA强阴离子离子交换色谱柱适用于各种蛋白质、核酸类的分离。

一)产品规格

YMC-BioPro QA

基材

亲水性聚合物

颗粒直径

5um

微孔径

100nm

官能团

-CH2N+(CH3)3

出厂时对离子

Cl-

离子交换容量

0.075-0.100 meq/mL-resin

动态吸附容量

110-150mg-BSA/mL-resin

温度范围

4-60℃

pH值范围

2-12

柱的材质

PEEK

二)产品特点

1)采用新开发的亲水性聚合物基材,使得蛋白质的非特异吸附降至极低;

2)通过最紧密的装填技术,实现了以往没有的高理论塔板数和的峰形对称性以及的分离能力;

3)具有高的动态吸附容量和高的回收率;

4)对于少量制备也非常有效;

三)产品应用

    人血清           牛血清白蛋白的胰蛋白酶

多孔型YMC-BioPro QA系列离子交换色谱柱

四)产品订购信息

填料

颗粒直径(μm)

微孔径(nm)

柱尺寸 [内径×长度(mm)]

产品编号

QA

S-5

100

4.6×50

QAA0S05-0546WP

多孔型YMC-BioPro QA系列离子交换色谱柱

1,4-b-D-葡聚糖纤维二糖水解酶[单胞菌] β-D-Xylosidase (Selenomonas ruminantium) 货号:E-BXSR-3KU Megazyme中文站

1,4-b-D-葡聚糖纤维二糖水解酶[单胞菌]

英文名:β-D-Xylosidase (Selenomonas ruminantium)

货号:E-BXSR-3KU

规格:3000 units

市场价: 3400

High purity recombinant exo-1,4-beta-D-Xylosidase (S. ruminantium) for use in research, biochemical enzyme assays andin vitro diagnostic analysis.

EC 3.2.1.37 
CAZy Family: GH43

1,4-β-D-xylan xylohydrolase.

Recombinant from S. ruminantium. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 115 U/mg (40oC, pH 5.3 on pNP-β-D-xylanopyranoside).

Data booklets for each pack size are located in the Technical Resources tab.

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XBridge?分析柱

XBridge™分析柱

XBridge™色谱柱专门为液相色谱方法开发和快速纯化而设计,用户能够在整个pH范围内使用种类多样的流动相体系和很宽的温度范围进行方法开发,从而建立稳定耐用的分析方法。XBridge OBD™制备色谱柱在高pH条件下分离和纯化碱性化合物能够得到更高的纯度,并且上样量可提高几十倍。

XBridge色谱柱具有非常稳定的性能和更长的寿命,即使在最苛刻的条件下。它采用革命性的创新技术生产,而且按照行业内最严苛的质量标准进行质量控制,因此成为用户针对挑战性色谱分离的值得信赖的解决方案。

XBridge色谱柱具有如下优势

➣ HPLC方法无间隙转换到UPLC ®

➣ 极端 pH条件下的更长的寿命和可靠性

➣ OBD制备柱的卓越性能

XBridge™ 色谱柱键合相

XBridge

C18

C8

Shield RP18

Phenyl

HILIC

键合相类型

三键键合 C18

三键键合 C8

单点键合 极性内嵌

三键键合 苯己基

未键合的 BEH颗粒

配体密度*

3.1 μmol/m2

3.2 μmol/m2

3.3 μmol/m2

3.0 μmol/m2

n/a

碳载量*

18%

13%

17%

15%

未键合

封端类型

特有

特有

TMS

特有

n/a

USP类别

L1

L7

L1

L11

L3

pH范围

1-12

1-12

2-11

1-12

1-9

低pH温度限值:

80 ℃

60 ℃

50 ℃

80 ℃

45 ℃

高pH温度限值:

60 ℃

60 ℃

45 ℃

60 ℃

45 ℃

孔径*

130 Å

130 Å

130 Å

130 Å

130 Å

表面积*

185 m2/g

185 m2/g

185 m2/g

185 m2/g

185 m2/g

粒径

2.5, 3.5, 5, 10 μm

2.5, 3.5, 5, 10 μm

2.5, 3.5, 5, 10 μm

2.5, 3.5, 5 μm

2.5, 3.5, 5 μm

TSKgel Protein A-5PW色谱柱

TSKgel Protein A-5PW色谱柱

是一款键合有重组Protein A配基的亲和色谱柱。

产品特点:

1、快速定量IgG

2分钟完成分析,在标准流速2.0 mL/min下。

(1分钟完成分析@4.0 mL/min)

2、很宽的IgG定量范围

非常好的线性,0.1-10 g/L IgG/柱(2-200 ug),R2>0.999。

3、耐用性好

连续2,000次进样后,色谱柱的表现并没有降低。

4、与TOYOPEARL AF-rProtein A HC-650F填料有相同的分离选择性。

产品描述

产品名称

TSKgel Protein A-5PW

货号

0023483

色谱柱尺寸

4.6 mm I.D. x 3.5 cm

色谱柱管材

PEEK

*没有保护柱

规格:

理论塔板数

280<=

不对称因子

0.6-1.5

填料属性:

基质

亲水性多孔聚合物

粒径

20 μm

粒径分布

15 – 25 μm 

孔径

100 nm

排阻上限

1,000 kDa

官能团

重组Protein A,Hexamer of C domain

官能团密度(代表值)

0.9 – 1.0 g/L-gel 

异潘糖 Isopanose – 100 mg 货号:O-IPAN Megazyme中文站

异潘糖

英文名:Isopanose – 100 mg

货号:O-IPAN

规格:100 mg

市场价: 2800

CAS: 82838-47-9
Molecular Formula: C18H32O16
Molecular Weight: 504.4
Purity: > 95%



High purity isopanose (6-O-α-D-maltosyl-glucosose) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis. Isopanose is formed by the action of isopullulanase on pullulan. It can be used as an analytical standard or as a substrate to help characterise the activities of other starch degrading enzymes including α-glucosidase and amyloglucosidase.

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ChromolithTM 整体化填料的色谱柱

ChromolithTM 整体化填料的色谱柱

ChromolithTM 整体化色谱柱是利用极高纯度且不含金属的烷氧基硅胶为材料. 采用新的液溶胶技术制备得到的整体化的多孔硅胶棒. 这种硅胶棒具有典型的孔结构. 即: 大孔结构和中孔结构. 其中大孔的孔径为2μm, 相互交联成密密麻麻的大孔网络. 保证流动相可以快速通过, 却不会造成大的反压. 填料骨架上13nm的中孔保证了填料具有比颗粒型色谱柱更高的比表面积. 从而可以使该色谱柱在保证高柱效和低反压的条件下使用高流速

特点:

快速平衡 , 仅需1-3分钟即可达到平衡

高流速 , 低压力, 流速可达9ml/min, 而不会产生高柱压问题

永远不会发生”柱头塌陷”, 通过变化流速改善分离状况

真正实现”流速梯度”, 通过变化流速改善分离状况

柱效与 3.5μm粒径的颗粒性色谱柱相当

总孔率在 80%以上

ChromolithTM 填料参数

填料名称

填料形态

孔径

比表面积

孔体积

碳含量

封端

PH 范围

RP-8e

整体化

2μm,13nm

300m2/g

1ml/g

11.0%

2~7.5

RP-18e

整体化

2μm,13nm

300m2/g

1ml/g

18.0%

Si

整体化

2μm,13nm

300m2/g

1ml/g

——

L-苹果酸检测试剂盒 L-Malic Acid Assay Kit (Manual Format) 货号:K-LMAL-58A Megazyme中文站

L-苹果酸检测试剂盒

英文名:L-Malic Acid Assay Kit (Manual Format)

货号:K-LMAL-58A

规格:58 assays per kit

市场价: 2000

L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.

Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

  • PVP incorporated to prevent tannin inhibition
  • Both enzymes supplied as stable suspensions
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction (~ 3 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual and microplate format

Q1. What is the difference between K-LMAL-58A / 116A, K-LMALAF, K-LMALMQ and K-LMALQR?

Megazyme produces 4 L-malic acid test kits:
K-LMAL-58A / 116A: UV method, automated format for use with auto-analysers.
K-LMALAF: UV method, manual format for use with spectrophotometers.
K-LMALMQ: Colourimetric method, manual format for use with hand held colorimeter.
K-LMALQR: UV method, liquid ready reagents automated format for use with auto-analysers.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q5. Which L-Malic Acid Kit is recommended for a 96-well microplate format?

Auto-analysers use ~ 0.315 mL reaction volumes and pathlengths between 4-8 mm which is similar to a standard 96-well microplate where a 0.315 mL reaction volume would give a pathlength of ~ 6-7 mm.  Therefore, K-LMALAF can be used directly in a 96-well microplate format with minimal assay optimisation.
If preferred, K-LMAL-58A / 116A may also be easily converted for use in a 96-well microplate format.  Basically, the assay volumes for the cuvette format must be reduced approximately 10-fold for use in a 96-well microplate.  However, some assay optimisation may be required (e.g. increased enzyme concentration etc.) and unlike the cuvette which has a set pathlength of 1 cm, the pathlength in the microplate is dependent upon the volume of liquid in the well.
Therefore to enable the calculation of the amount of analyte in the samples from tests performed in the microplate format one of the following must be done:

  1. The easiest method is to use a microplate reader that has a pathlength conversion capability (i.e. the microplate reader can detect the pathlength of each well and convert the individual readings to a 1 cm pathlength).  This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm pathlength) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values.

L-Malic Acid Kit Recommendation For Microplate Format:
Either K-LMAL-58A / 116A or K-LMALAF is recommended for use in a 96-well microplate format and the main advantages / disadvantages are described below:
K-LMAL-58A / 116A:
The assay volumes of this kit should be reduced by 10-fold for use in a 96-well microplate format (some assay optimisation may be required, e.g. increased enzyme concentration etc.).
The calculation of results is achieved as outlined above in either of points 1, 2 or 3.
The main advantage here is that if this kit is used with a microplate reader that has a pathlength conversion capability, or if results are converted as outlined above in point 3, then this enables easy calculation of results using the K-LMAL-58A / 116A MegaCalc application (available on the Megazyme website where the product is located).
K-LMALAF:
This kit is designed for use in an auto-analyser and therefore can be used without any modification to assay volumes directly in a 96-well microplate format. 
This kit has less reagent additions than K-LMAL-58A / 116A.
K-LMALAF does not have a MegaCalc application available to enable easy results calculation which therefore must be achieved as outlined above in either of points 2 or 3.

Q6. Do samples require any specific sample preparation prior to testing with the kits?

The sample preparation is sample dependent, some samples may be tested directly in the assay or after appropriate dilution, however, some samples may require further sample preparation prior to testing.  The following are example of sample preparation methods:
(a) Liquid samples: clear, slightly coloured and approximately neutral, liquid samples can be used directly in the assay.
(b) Acidic samples: if > 0.1 mL of an acidic sample is to be used undiluted (such as wine or fruit juice), the pH of the solution should be increased to approx. 9.0 using 2 M NaOH, and the solution incubated at room temperature for 30 min.
(c) Carbon dioxide: samples containing significant quantities of carbon dioxide, such as beer, should be degassed by increasing the pH to approx. 9.0 with 2 M NaOH and gentle stirring, or by stirring with a glass rod.
(d) Coloured samples: an additional sample blank, i.e. sample with no L-MDH, may be necessary in the case of coloured samples.
(e) Strongly coloured samples: if used undiluted, strongly coloured samples should be treated by the addition of 0.2 g of PVPP/10 mL of sample.  Shake the tube vigorously for 5 min and then filter through Whatman No. 1 filter paper.
(f) Solid samples: homogenise or crush solid samples in distilled water and filter if necessary.
(g) Samples containing fat: extract such samples with hot water at a temperature above the melting point of the fat, e.g. in a 100 mL volumetric flask.  Adjust to room temperature and fill the volumetric flask to the mark with distilled water.  Store on ice or in a refrigerator for 15-30 min and then filter.  Discard the first few mL of filtrate, and use the clear supernatant (which may be slightly opalescent) for assay. Alternatively, clarify with Carrez reagents.
(h) Samples containing protein: deproteinise samples containing protein by adding an equal volume of ice-cold 1 M perchloric acid with mixing.  Centrifuge at 1,500 g for 10 min and neutralise the supernatant with 1 M KOH.  Alternatively use Carrez reagents.

Q7. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the 
K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch 
this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q8. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

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Megazyme L-苹果酸(手动)检测试剂盒操作视频(K-LMAL)

Megazyme 溶解淀粉 操作视频

Megazyme 试剂盒样品前处理准备操作视频

Ultra Aqueous C18色谱柱

Ultra Aqueous C18

产品描述:

粒径(μm):3、5,球型

孔径(Å):100

碳载量(%):15

封尾:否

PH范围:2.5 – 8

温度上限(°C):80

对极性化合物有强保留和选择性。高度碱去活。适用于纯水流动相

货号

粒径

柱长

内径

包装量

917835B

3μm

50mm

0.3mm

ea.

917836B

3μm

150mm

0.3mm

ea.

9178331

3μm

30mm

1.0mm

ea.

9178351

3μm

50mm

1.0mm

ea.

9178311

3μm

100mm

1.0mm

ea.

9178361

3μm

150mm

1.0mm

ea.

9178332

3μm

30mm

2.1mm

ea.

9178352

3μm

50mm

2.1mm

ea.

9178312

3μm

100mm

2.1mm

ea.

9178362

3μm

150mm

2.1mm

ea.

917833E

3μm

30mm

3.0mm

ea.

917835E

3μm

50mm

3.0mm

ea.

917831E

3μm

100mm

3.0mm

ea.

917836E

3μm

150mm

3.0mm

ea.

9178333

3μm

30mm

3.2mm

ea.

9178353

3μm

50mm

3.2mm

ea.

9178313

3μm

100mm

3.2mm

ea.

9178363

3μm

150mm

3.2mm

ea.

9178335

3μm

30mm

4.6mm

ea.

9178355

3μm

50mm

4.6mm

ea.

9178315

3μm

100mm

4.6mm

ea.

9178365

3μm

150mm

4.6mm

ea.

9178531

5μm

30mm

1.0mm

ea.

9178551

5μm

50mm

1.0mm

ea.

9178511

5μm

100mm

1.0mm

ea.

9178561

5μm

150mm

1.0mm

ea.

9178521

5μm

200mm

1.0mm

ea.

9178571

5μm

250mm

1.0mm

ea.

9178532

5μm

30mm

2.1mm

ea.

9178552

5μm

50mm

2.1mm

ea.

9178512

5μm

100mm

2.1mm

ea.

9178562

5μm

150mm

2.1mm

ea.

9178522

5μm

200mm

2.1mm

ea.

9178572

5μm

250mm

2.1mm

ea.

9178533

5μm

30mm

3.2mm

ea.

9178553

5μm

50mm

3.2mm

ea.

9178513

5μm

100mm

3.2mm

ea.

9178563

5μm

150mm

3.2mm

ea.

9178523

5μm

200mm

3.2mm

ea.

9178573

5μm

250mm

3.2mm

ea.

9178535

5μm

30mm

4.6mm

ea.

9178555

5μm

50mm

4.6mm

ea.

9178515

5μm

100mm

4.6mm

ea.

9178565

5μm

150mm

4.6mm

ea.

9178525

5μm

200mm

4.6mm

ea.

9178575

5μm

250mm

4.6mm

ea.

D-果糖和D-葡萄糖测定试剂盒(MegaQuant™ Format) The D-Fructose and D-Glucose Assay Kit (MegaQuant™ Format) 货号:K-FRGLMQ Megazyme中文站

D-果糖和D-葡萄糖测定试剂盒(MegaQuant™ Format)

英文名:The D-Fructose and D-Glucose Assay Kit (MegaQuant™ Format)

货号:K-FRGLMQ

规格:60 assays per kit

市场价: 3200

The D-Fructose and D-Glucose MegaQuant™ Format test kit is suitable for the measurement and analysis of D-fructose and D-glucose in grapes, grape juice and wine using the MegaQuant™ colorimeter (measurement at 505 nm).

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and   therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q5. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q6. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

无孔型 YMC-BioPro SP-F离子交换色谱柱

无孔型 YMC-BioPro SP-F离子交换色谱柱

无孔型YMC-BioPro SP-F强阳离子离子交换色谱柱适用于各种蛋白质、核酸类的分离。

一)产品规格

YMC-BioPro SP-F

基材

亲水性聚合物

颗粒直径

5um

微孔径

Non-porous

官能团

-CH2CH2CH2S03-

出厂时对离子

Na+

离子交换容量

0.230-0.290 meq/mL-resin

动态吸附容量

10-18mg-human-lgG/mL-resin

温度范围

4-60℃

pH值范围

2-12

柱的材质

PEEK

二)产品特点

1)采用新开发的亲水性聚合物基材,使得蛋白质的非特异吸附降至极低;

2)通过最紧密的装填技术,实现了以往没有的高理论塔板数和峰形对称性;

3)实现了最小限度的压力损失和高分离能力;

4)有对于超高速分析十分有效的30mm柱及具有极高分离能力的100mm柱;

三)产品应用

1)蛋白质的超高速分析

无孔型 YMC-BioPro SP-F离子交换色谱柱

2)高理论塔板数和的峰形对称性0

无孔型 YMC-BioPro SP-F离子交换色谱柱

四)产品订货信息

填料

颗粒直径(μm)

微孔径(nm)

柱尺寸 [内径×长度(mm)]

产品编号

SP-F

S-5

Non-porous

4.6×30

SF00S05-0346WP

4.6×50

SF00S05-0546WP

4.6×100

SF00S05-1046WP

无孔型 YMC-BioPro SP-F离子交换色谱柱

Inertsil ODS-EP

Inertsil ODS-EP

极性基团键合十八烷基的嵌入型ODS色谱柱

Inertsil ODS-EP

Inertsil ODS-EP在十八烷基的根部导入了极性官能团。由于嵌入的极性基团化合物与氢键结合,所以这款色谱柱有着容易选择地保留极性化合物的特长。

独特地选择性

Inertsil ODS-EP提供了独特地选择性,是由于嵌入的极性基团化合物与氢键结合的相互作用。

当通过其他分离机制,如疏水相互作用或π-π相互作用不能实现分离时,推荐Inertsil ODS-EP。

下图显示了使用Inertsil ODS-EP, Inertsil Ph-3,Inertsil C8-3时,中性,酸性和碱性化合物的溶出。

Inertsil ODS-EP

无论是酸性化合物还是碱性化合物都能得到很好的峰形

一般市场上销售的极性嵌合选择性色谱柱中,导入的极性基团大部分是含氮官能团,所以由于酸性化合物的吸附作用,而造成峰形变差以及在酸性洗脱液中稳定性较差的缺点。通过嵌入适当的极性基团,Inertsil ODS-EP能够很好地分离酸性化合物和碱性化合物,从而得到很好的峰形。在下图中,显示了分别使用Inertsil ODS-EP,Inertsil Ph-3和Inertsil ODS-3在酸性条件下酸性化合物的分离情况。从而得知,只有Inertsil ODS-EP能够对那些结构相似的酸性化合物进行彻底的分离。

Inertsil ODS-EP

在高水相流动相中的稳定保留

嵌入的极性基团有助于弄湿疏水链并防止”流动相崩塌”。 因此,即使使用100%的水相洗脱液的情况下,Inertsil ODS-EP也能与高度水性洗脱液兼容。

以下的色谱图比较了Inertsil ODS-EP和纯烷基C18键合相的保留性。无水的惰性物质的保留率不受百分之百的水的影响。表明了即使在100%的水相洗脱液的情况下,Inertsil ODS-EP不受影响。

Inertsil ODS-EP

规格

Inertsil ODS-EP

1:3,1:4-β-D-低聚糖的纤维素(B) 1,3:1,4 BETA-Glucotriose (B) 50mg 货号:O-BGTRIB Megazyme中文站

1:3,1:4-β-D-低聚糖的纤维素(B)

英文名:1,3:1,4 BETA-Glucotriose (B) 50mg

货号:O-BGTRIB

规格:50 mg

市场价: 3000

CAS: 32581-36-5
Molecular Formula: C18H32O16
Molecular Weight: 504.4
Purity: > 95%

High purity 1,3:1,4-β-Glucotriose (B) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Trisaccharide produced on hydrolysis of 1:3,1:4-β-D-glucan by lichenase. Glc1-4Glc1-3Glc

暂无问题解答

暂无视频

MXT-5

MXT-5

弱极性固定相。Crossbond 5%二苯基/95% 二甲基聚硅氧烷

通用型色谱柱。可用于药物、溶剂杂质、杀虫剂、烃、多氯联苯类、香精油、半挥发物的分析

温度范围:-60°C – 430°C

相当于USP G27和G36 固定相

通过特殊工艺将固定相中残留的催化剂和低分子量杂质除去,使该固定相仅以键和基团发挥吸附作用,并表现出极低的柱流失

相似固定相:DB-5、HP-5、HP-5MS、Ultra-2、SPB-5、Equity-5、MDN-5、CP-Sil 8 CB

货号

长度

内径

膜厚

架子直径

包装量

71821

10m

0.18mm

0.20μm

ea.

71822

20m

0.18mm

0.20μm

ea.

71823

40m

0.18mm

0.20μm

ea.

71824

10m

0.18mm

0.40μm

ea.

71825

20m

0.18mm

0.40μm

ea.

71826

40m

0.18mm

0.40μm

ea.

70205

15m

0.25mm

0.10μm

ea.

70208

30m

0.25mm

0.10μm

ea.

70211

60m

0.25mm

0.10μm

ea.

70220

15m

0.25mm

0.25μm

ea.

70223

30m

0.25mm

0.25μm

ea.

70226

60m

0.25mm

0.25μm

ea.

70235

15m

0.25mm

0.50μm

ea.

70238

30m

0.25mm

0.50μm

ea.

70241

60m

0.25mm

0.50μm

ea.

70250

15m

0.25mm

1.00μm

ea.

70253

30m

0.25mm

1.00μm

ea.

70256

60m

0.25mm

1.00μm

ea.

70221

15m

0.28mm

0.25μm

ea.

70224

30m

0.28mm

0.25μm

ea.

70227

60m

0.28mm

0.25μm

ea.

70236

15m

0.28mm

0.50μm

ea.

70239

30m

0.28mm

0.50μm

ea.

70242

60m

0.28mm

0.50μm

ea.

70251

15m

0.28mm

1.00μm

ea.

70254

30m

0.28mm

1.00μm

ea.

70257

60m

0.28mm

1.00μm

ea.

70281

15m

0.28mm

3.00μm

ea.

70284

30m

0.28mm

3.00μm

ea.

70287

60m

0.28mm

3.00μm

ea.

70222

15m

0.53mm

0.25μm

ea.

70225

30m

0.53mm

0.25μm

ea.

70228

60m

0.53mm

0.25μm

ea.

70237

15m

0.53mm

0.50μm

ea.

70240

30m

0.53mm

0.50μm

ea.

70243

60m

0.53mm

0.50μm

ea.

70252

15m

0.53mm

1.00μm

ea.

70252-273

15m

0.53mm

1.00μm

3.5英寸

ea.

70255

30m

0.53mm

1.00μm

ea.

70255-273

30m

0.53mm

1.00μm

3.5英寸

ea.

70258

60m

0.53mm

1.00μm

ea.

70267

15m

0.53mm

1.50μm

ea.

70270

30m

0.53mm

1.50μm

ea.

70273

60m

0.53mm

1.50μm

ea.

70282

15m

0.53mm

3.00μm

ea.

70285

30m

0.53mm

3.00μm

ea.

70288

60m

0.53mm

3.00μm

ea.

70277

15m

0.53mm

5.00μm

ea.

70279

30m

0.53mm

5.00μm

ea.

70283

60m

0.53mm

5.00μm

ea.

YMC-BioPro QA-F离子交换色谱柱

无孔型YMC-BioPro QA-F离子交换色谱柱

无孔型YMC-BioPro QA-F强阴离子离子交换色谱柱适用于各种蛋白质、核酸类的分离。

一)产品规格

YMC-BioPro QA-F

基材

亲水性聚合物

颗粒直径

5um

微孔径

Non-porous

官能团

-CH2N+(CH3)3

出厂时对离子

Cl-

离子交换容量

0.075-0.110 meq/mL-resin

动态吸附容量

12-20mg-BSA/mL-resin

温度范围

4-60℃

pH值范围

2-12

柱的材质

PEEK

二)产品特点

1)采用新开发的亲水性聚合物基材,使得蛋白质的非特异吸附降至极低;

2)通过最紧密的装填技术,实现了以往没有的高理论塔板数和峰形对称性;

3)实现了最小限度的压力损失和高分离能力;

4)有对于超高速分析十分有效的30mm柱及具有极高分离能力的100mm柱;

三)产品应用

1)抗人体lgG4单克隆抗体(Mab)

YMC-BioPro QA-F离子交换色谱柱

2)DNA片段1kb DNA ladder(75-12,216 bp)

注:采用长度100mm和30mm柱来比较DNA片段的分离

YMC-BioPro QA-F离子交换色谱柱

四)产品订货信息

填料

颗粒直径(μm)

微孔径(nm)

柱尺寸 [内径×长度(mm)]

产品编号

QA-F

S-5

Non-porous

4.6×30

QF00S05-0346WP

4.6×50

QF00S05-0546WP

4.6×100

QF00S05-1046WP

YMC-BioPro QA-F离子交换色谱柱

Lipase脂肪酶, 质谱级

产品信息|Certificate of Analysiss

Lipase脂肪酶, 质谱级

订货号

产品名称

包装

HLS LIP001C

Lipase 脂肪酶

50µg

说明: 脂肪酶可催化水溶液中三酰甘油的水解,生成甘油和游离脂肪酸。该脂肪酶来源于猪胰腺。

在PBS缓冲液或Tris-HCL缓冲液中,Triglyceride + H2O -> Diglyceride + Fatty Acid。

物理形态: 质谱级Lipase 脂肪酶是冻干粉

分子量: 48kDa.

溶解液:Lipase 脂肪酶用100ul 5mM氯化钙溶解(使用前冰浴)

储存条件:冻干粉末存储在-20℃冰箱,再溶解的酶储存在-20℃。有效期见产品标签

保质期:12月在-20℃

pH 范围: 在 PH=7.7 时, Lipase脂肪酶具有很好活性

适用范围:Lipase脂肪酶特异性水解血清或动物组织样品中的三酰甘油。

溶液酶解SOP(仅供参考):

20ul 血清样本加20ul 100mM PBS buffer稀释PH=7.7, 37℃保温30min,立即加入2-6ul Lipase 脂肪酶保温15min

质量控制|Quality Control

纯度: 液相色谱280nm检测杂质蛋白质峰强度,脂肪酶峰强度大于99.0%

活性:20000unit/mg;

单位定义:在PH 7.7、温度37 °C条件下,以橄榄油为底物,在1小时内水解甘油三酸酯而释放1.0微当量的脂肪酸所需的酶量

LC-MS/MS 质谱分析: N/A

L-阿拉伯糖/ D-半乳糖测定试剂盒 L-Arabinose/D-Galactose Assay Kit 货号:K-ARGA Megazyme中文站

L-阿拉伯糖/ D-半乳糖测定试剂盒

英文名:L-Arabinose/D-Galactose Assay Kit

货号:K-ARGA

规格:115 assays (manual) / 1150 assays (microplate)

市场价: 4000

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products. 

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC. 

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of L-Arabinose and D-Galactose 
in hydrolysed plant products

Principle:
                                      (galactose mutarotase)
(1) α-L-Arabinose / α-D-galactose ↔ β-L-arabinose / β-D-galactose

                      (β-galactose dehydrogenase)
(2) β-L-Arabinose + NAD+ → L-arabinonic acid + NADH + H+
                     
                    (β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

 

Kit size:                            115 assays (manual) / 1150 (microplate)
                                          / 1150 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                   ~ 5 min
Detection limit:                 1.3 mg/L
Application examples:
Analysis of hydrolysates of oligo- and polysaccharides (e.g. arabinan,
arabinoxylan, galactan, arabinogalactan), milk, dairy products, foods
containing milk (e.g. dietetic foods, bakery products, baby food,
chocolate, sweets and ice-cream), food additives (e.g. sweeteners),
cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:       Novel method

 Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and   therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q5. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q6. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q7. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q8. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q9. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q10. Can oligosaccharides or polysaccharides be measured using the kit assay?

The kit assay will only measure the non-covalently linked monosaccharide.

Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.

Q11. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q12. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q13. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q14. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

疏水层析填料

疏水层析填料

生物分子表面大都含或强或弱的疏水区域,在不同环境下,与各种疏水介质产生不同强弱和结合。和离子交换刚刚相反,疏水填料高盐吸附,低盐洗脱。经洗脱的样品可直接或稍加稀释后上其它层析柱,做为连接下游和层析步骤的桥梁,并可完全取代传统的盐析沉淀技术,更符合工业生产要求。疏水层析填料比反相层析介质的配体密度低很多,无需有机溶剂洗脱,保存生物活性。配体种类繁多,提供宽广的选择性。

                           

基架

粗分离

中度纯化

精细纯化

Phenyl Sepharose 6 Fast Flow(High Sub)

Phenyl Sepharose 6 Fast Flow(Low Sub)

Octyl Sepharose 4 Fast Flow

Butyl Sepharose 4 Fast Flow

Phenyl Sepharose High Performance

SOURCE 15 PHE,ISO,ETH

一、预处理及中度纯化介质

二、中度分离及最终纯化介质

三、经典疏水层析介质

Alpha葡萄糖醛酸酶[分离嗜热菌] α-Glucuronidase (Geobacillus stearothermophilus) (Recombinant) 货号:E-AGUBS Megazyme中文站

Alpha葡萄糖醛酸酶[分离嗜热菌]

英文名:α-Glucuronidase (Geobacillus stearothermophilus) (Recombinant)

货号:E-AGUBS

规格:200 Units at 70°C

市场价: 2600

High purity recombinant alpha-D-Glucuronidase (Geobacillus stearothermophilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.139
CAZy Family: GH67

Recombinant from Geobacillus stearothermophilus. 
In 3.2 M ammonium sulphate.
Hydrolysis of the α-1,2 glycosidic bond between D-glucuronic acid or it’s ether 4-O-methyl-D-glucuronic acid and 
D-xylose residues of xylo-oligosaccharides (aldouronic acids) from xylan.

Specific activity: ~ 7 U/mg (70oC, pH 7.0 on aldouronic acid).

Stability > 2 years 4oC.

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COSMOSIL Buckyprep

制备分离富勒烯通常应用色谱方法,但是分离效果不好, COSMOSIL Buckyprep 填充柱专为富勒烯的HPLC制备分离而设计。分析柱尺寸的负载量与传统C18制备柱的载量相同,所以使用HPLC分析系统可制备分离富勒烯,因COSMOSIL Buckyprep色谱柱化学键合硅胶相,所以不需要特殊的操作技术。

填料特性

COSMOSIL ® Buckyprep

硅胶

高纯多孔球形硅胶

平均粒径

5µm

平均孔径

120Å

比表面积

300m²/g

固定相

3-(1- 芘基 ) 丙基

封端

几乎完全封端

碳含量

17%

建议溶剂:甲苯、氯苯、1,2,4-三氯苯、正己烷。

应用实例: BUCKYPREP色谱柱分离高富勒烯

COSMOSIL Buckyprep

订货信息

产品名称

尺寸

货号

COSMOSIL ® 5Buckyprep 保护柱

4.6mmI.D.x 10mm

37983-71

10mmI.D.x 20mm

37984-61

20mmI.D.x 50mm

34374-41

COSMOSIL ® 5Buckyprep 填充柱

4.6mmI.D.x 250mm

37977-61

10mmI.D.x 250mm

37981-91

20mmI.D.x 250mm

37982-81

DB-17ms柱

DB-17ms柱

特点

· 实际上相当于(50%-苯基)-甲基聚硅氧烷

· 温度上限为320/340℃

· 极低的流失和中极性十分适合于GC/MS

· 对于活性化合物都呈现优异的惰性

· 质谱谱图更完整

· 键合交联且可用溶剂冲洗

· 分析CLP杀虫剂的最好色谱柱

Allure Aqueous C18色谱柱

Allure Aqueous C18

产品描述:

粒径(μm):5,球型

孔径(Å):60

封尾:否

PH范围:2.5 – 8

温度上限(°C):80

对极性化合物,包括酸性化合物有很好的选择性

适用于纯水流动相。高度碱去活。配合LC/MS检测器,适用于分析宽范围化合物

选择保护柱内置型色谱柱,请在货号后添加-700

货号

柱长

内径

包装量

9168531

30mm

1.0mm

ea.

9168551

50mm

1.0mm

ea.

9168511

100mm

1.0mm

ea.

9168561

150mm

1.0mm

ea.

9168521

200mm

1.0mm

ea.

9168571

250mm

1.0mm

ea.

9168532

30mm

2.1mm

ea.

9168552

50mm

2.1mm

ea.

9168512

100mm

2.1mm

ea.

9168562

150mm

2.1mm

ea.

9168522

200mm

2.1mm

ea.

9168572

250mm

2.1mm

ea.

9168533

30mm

3.2mm

ea.

9168553

50mm

3.2mm

ea.

9168513

100mm

3.2mm

ea.

9168563

150mm

3.2mm

ea.

9168523

200mm

3.2mm

ea.

9168573

250mm

3.2mm

ea.

9168535

30mm

4.6mm

ea.

9168555

50mm

4.6mm

ea.

9168515

100mm

4.6mm

ea.

9168565

150mm

4.6mm

ea.

9168525

200mm

4.6mm

ea.

9168575

250mm

4.6mm

ea.

阿拉伯树胶六糖[纯度>95%][粉] Arabinohexaose (powder) – 20mg 货号:O-AHE Megazyme中文站

阿拉伯树胶六糖[纯度>95%][粉]

英文名:Arabinohexaose (powder) – 20mg

货号:O-AHE

规格:20 mg

市场价: 2300

CAS: 190852-26-7 
Molecular Formula: C30H50O25
Molecular Weight: 810.7
Purity: > 95%



High purity Arabinohexaose (powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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InertSustain Phenyl

InertSustain Phenyl

苯基柱提供π相互作用和形状选择性

InertSustain Phenyl

InertSustain系列

我公司为InertSustain系列开发了新型ES硅胶,可提供高惰性及高耐久性。具备惰性和持久性的InertSustain 系列HPLC色谱柱是我公司最新的技术,也被推荐为各种分析的首选。

当使用C8色谱柱或C18色谱柱不能充分分离时,InertSustain Phenyl是个理想的选择。nertSustain Phenyl具有π电子相互作用,因此能够保留极性化合物。此外,它也有优异的立体选择性,所以还可用于分析同分异构体。

π电子相互作用的选择性

普通的苯基柱是苯基烷基键合在硅胶上,而InertSustain Phenyl是直接在硅胶上键合苯基。因此,与普通的苯基柱相比,InertSustain Phenyl的分离机理更依赖于π电子相互作用,从而提高了对芳香族化合物的选择性。

高立体选择性

InertSustain Phenyl有很高的立体选择性是因为苯基的高密度键合。InertSustain Phenyl可分离同分异构体包括位置异构体。

InertSustain Phenyl

InertSustain Phenyl

规格

InertSustain Phenyl