转化酶(粉末)[酵母] Invertase Powder 货号:E-INVPD-5G Megazyme中文站

转化酶(粉末)[酵母]

英文名:Invertase Powder

货号:E-INVPD-5G

规格:5 grams

市场价: 3800

High purity Invertase (powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.26

β-Fructofuranosidase. Sucrase. Saccharase.

Specific activity: 300 U/mg of solid; 900 U/mg protein.

Data booklets for each pack size are located in the Technical Resources tab.

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Beta甘露糖苷酶[纤维单胞菌] Beta-Mannosidase (C. fimi) – 200U 货号:E-BMOSCF Megazyme中文站

Beta甘露糖苷酶[纤维单胞菌]

英文名:Beta-Mannosidase (C. fimi) – 200U

货号:E-BMOSCF

规格:200 Units

市场价: 3200

High purity recombinant beta-Mannosidase (C. fimi) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.25 
CAZy Family: GH2

Recombinant from Cellulomonas fimi. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 15 U/mg (35oC; 0.8 mM p-nitrophenyl β-D-mannopyranoside; pH 6.5).

Stable at 4oC for > 2 years.

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TSK-GEL 亲和层析柱(AFC)

TSK-GEL 亲和层析柱(AFC)

TOSOH 公司出品的TSK-GEL亲和色谱系列有三种特殊基团的固定相和一种活性填料Tresyl-5PW组成。亲和色谱对生物分子的分离和纯化给出高水平的特殊性和选择性。种类有分析、制备及工艺规模的亲和色谱。

TSK-GEL亲和色谱柱以著名的G5000PW多孔树脂为基体,此树脂也是高性能尺寸排阻色谱的基体。5PW基体树脂是一种亲水性介质,孔尺寸为1000A,蛋白质排出极值估计为5×106Da。TOSOH公司的工艺规模的亲和介质为65μm粒径,半刚性Toyopearl HW-65树脂。因为分析和半制备柱和工艺规模介质有同样的聚合物化学,因此从实验室到工艺规模,可以达到按比例准确扩大。

特 性

优 点

高尺寸排阻限值 (1000 Å )

促进了亲和色谱对大分子蛋白质的分离

小颗粒尺寸

分析柱 (10μm)和半制备柱(13μm)亲和的高效应用

刚性聚合物基体树脂

宽广的 PH使用范围,可以进行清洗

稳定的亲和配合体

寿命长、溶剂相容性好、可高压灭菌

四种亲和配合体

应用灵活性强,能够从实验室过渡到商业生产

TSKgel BioAssist 螯合硬件为 PEEK 材料

减少与柱硬件之间的交互作用

TSK-GEL亲和色谱柱填料

TSK-GEL 亲和层析柱(AFC)

订货信息

货号

生产商

产品描述 ( IDmm×Lengthcm )

14452

TOSOH

ABA-5PW Glass,1000 Å ( 5.0×5.0 )

14453

TOSOH

ABA-5PW Glass,1000 Å ( 8.0×7.5 )

14449

TOSOH

Boronate-5PW Glass, 1000 Å ( 5.0×5.0 )

14450

TOSOH

Boronate-5PW Glass, 1000 Å ( 8.0×7.5 )

14440

TOSOH

Chelate-5PW Glass, 1000 Å ( 5.0×5.0 )

14441

TOSOH

Chelate-5PW Glass, 1000 Å ( 8.0×7.5 )

14443

TOSOH

Heparin-5PW Glass, 1000 Å ( 5.0×5.0 )

14444

TOSOH

Heparin-5PW Glass, 1000 Å ( 8.0×7.5 )

14458

TOSOH

Tresyl-5PW Glass ( 8.0×7.5 )

14457

TOSOH

Tresyl-5PW Glass ( 5.0×5.0 )

13067

TOSOH

ABA-5PW ,1000 Å ( 7.5×7.5 )

13066

TOSOH

Boronate-5PW, 1000 Å ( 7.5×7.5 )

08645

TOSOH

Chelate-5PW, 1000 Å ( 7.5×7.5 )

08645

TOSOH

Chelate-5PW, 1000 Å ( 21.5×15 )

13064

TOSOH

Heparin-5PW, 1000 Å ( 7.5×7.5 )

14455

TOSOH

Tresyl-5PW (6.0×4.0)

14456

TOSOH

Tresyl-5PW ( 7.5×7.5 )

20022

TOSOH

BioAssist Chelate, 1000 Å ( 7.8×5.0 )

14454

TOSOH

ABA-5PW Glass Guardgel Kit

13127

TOSOH

ABA-5PW Guardgel Kit

14451

TOSOH

Boronate-5PW Glass Guardgel Kit

13125

TOSOH

Boronate-5PW Guardgel Kit

14442

TOSOH

Chelate-5PW Glass Guardgel Kit

08647

TOSOH

Chelate-5PW Guardgel Kit

14445

TOSOH

Heparin-5PW Glass Guardgel Kit

13121

TOSOH

Heparin-5PW Guardgel Kit

08809

TOSOH

Tresyl-5PW Glass Guardge Holder

16208

TOSOH

Tresyl-5PW,2g dry gel*

*1g大约3.5mL

Silgreen C8色谱柱

SilGreen C8色谱柱选用超纯硅胶为填料基体,表面键合C8长链基团,充分封端的工艺使硅羟基的活性降到了最低,具有出色的惰性,耐久性和批次重现性等特点。疏水性相互作用比C18柱弱,其作为C18色谱柱的补充,可用于需要高速分析的农药、小肽等。

填料性能

填料

官能团

碳载量(%)

孔径

粒径

比表面积

pH

封端

USP

球形硅胶

C8

11

100 Å

3μm,5μm

400m2/g

2-7.5

完全封端

L7

订货信息

货号

孔径(Å)

粒径(um)

柱尺寸ID*L, mm

GH0525046C8A

100

5

4.6*250

GH0515046C8A

100

5

4.6*150

Toyopearl尺寸排除填料

Toyopearl尺寸排除填料

尺寸排阻色谱 (Size exclusion chromatography SEC), 也被称为凝胶过滤. 是根据物质尺寸大小的不同来进行分离. 一般情况下, 体积排阻层析可以用于纯化的一步,按分子量大小将产品粗分;也可以用作纯化的中间步骤来改换缓冲液或脱盐,还可以用于纯化的最后一步来去除杂质,精制产品。

特点:

➣ 孔径分布范围: 50Å->1000Å

➣ 三种粒径( S,F,C)

➣ HW-40用于脱盐是理想选择

➣ 易于装填半制备柱和制备柱

Toyopearl HW型填料特性和分子量排阻范围

Toyopearl填料

粒径μ m

孔径 Å

聚乙二醇类

环糊精

球形蛋白

HW-40S
HW-40F
HW-40C

20-40
30-60
50-100

50
50
50

102 – 3×103

102 – 7×103

102 – 10 4

HW-50S
HW-50F

20-40
30-60

125
125

102 – 1.8×104

5×102 -2×104

5×102 – 8×104

HW-55S
HW-55F

20-40
30-60

500
500

102 – 1.5×105

103 -2×105

103 – 7×105

HW-65S
HW-65F

20-40
30-60

1000
1000

5×102 – 106

104 – 106

4×104 – 5×106

HW-75F

30-60

>1000

4×103 – 2×106

105 – 1×107

5×105 – 5×107

TSK-GEL PW树脂采用与HW树脂相同的基材,交联度更高。 使用的填料粒径更小(≤30μm), 柱效更高,承受的压力可达20bar, 通常用于TSK分析柱的直接放大, 是中压液相层析的良好选择。 其与HW的对照如下表:

Toyopearl

TSK-GEL

孔径 (Å)

HW-75

6000PW

>1000

HW-65

5000PW

1000

HW-55

4000PW

400 – 500

HW-50

2000PW

125

HW-40

1000PW

50

订货信息

货号

生产商

产品描述

7469

TOSOH

HW-75F 500mL

8069

TOSOH

HW-65F 5L

8068

TOSOH

HW-65S 5L

8063

TOSOH

HW-55F 5L

8062

TOSOH

HW-55S 5L

8060

TOSOH

HW-50F 5L

8544

TOSOH

TOPOFFSWXL 1mL

7969

TOSOH

HW-40C 5L

14681

TOSOH

HW-40S 1L

8070

TOSOH

HW-65C 5L

7471

TOSOH

HW-75S 250mL

8059

TOSOH

HW-50S 5L

7468

TOSOH

HW-75S 500mL

14688

TOSOH

HW-65S 1L

14687

TOSOH

HW-55F 1L

14686

TOSOH

HW-55S 1L

14685

TOSOH

HW-50F 1L

14684

TOSOH

HW-50S 1L

14683

TOSOH

HW-40C 1L

14682

TOSOH

HW-40F 1L

7967

TOSOH

HW-40S 5L

19812

TOSOH

HW-55F 150mL

19814

TOSOH

HW-65F 150mL

19815

TOSOH

HW-65S 150mL

19816

TOSOH

HW-75F 150mL

7968

TOSOH

HW-40F 5L

19819

TOSOH

SECPAK HMW (HW55, 65, 75 F), 45μm

8071

TOSOH

HW-75S 5L

19820

TOSOH

SECPAK HP (HW40, 50, 55, 65S), 30μm

6819

TOSOH

TOPOFF SW

19813

TOSOH

HW-55S 150mL

19811

TOSOH

HW-50S 150mL

19810

TOSOH

HW-50F 150mL

19809

TOSOH

HW-40S 150mL

19808

TOSOH

HW-40F 150mL

19807

TOSOH

HW-40C 150mL

14691

TOSOH

HW-75F 1L

14689

TOSOH

HW-65F 1L

19821

TOSOH

ECPAK LMW ( HW-40, 50, 55 F), 45μm

19531

TOSOH

HW-65M 1L

18368

TOSOH

HW-50F 50L

8072

TOSOH

HW-75F 5L

8035

TOSOH

TOPOFFPWXL 1mL

7464

TOSOH

HW-65S 500mL

7449

TOSOH

HW-40C 500mL

7447

TOSOH

HW-40S 500mL

7448

TOSOH

HW-40F 500mL

7450

TOSOH

HW-40EC 500mL

7451

TOSOH

HW-40S 250mL

7452

TOSOH

HW-50S 500mL

7453

TOSOH

HW-50F 500mL

7455

TOSOH

HW-50S 250mL

7456

TOSOH

HW-55S 500mL

7459

TOSOH

HW-55S 250mL

7465

TOSOH

HW-65F 500mL

7466

TOSOH

HW-65C 500mL

7467

TOSOH

HW-65S 250mL

7457

TOSOH

HW-55F 500mL

1,4-β-D- Cellotetraitol(硼氢化还原纤维四糖) 1,4-β-D-Cellotetraitol (borohydride reduced cellotetraose) 货号:O-CTERD Megazyme中文站

1,4-β-D- Cellotetraitol(硼氢化还原纤维四糖)

英文名:1,4-β-D-Cellotetraitol (borohydride reduced cellotetraose)

货号:O-CTERD

规格:50 mg

市场价: 3000

CAS: 5548-55-0
Molecular Formula: C24H44O21
Molecular Weight: 668.6
Purity: > 95%

High purity 1,4-β-D-Cellotetraitol (borohydride reduced cellotetraose) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Tetrasaccharides from hydrolysis of cellulose and borohydride reduced.

Store at room temperature.

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D-葡萄糖[HK法]检测试剂盒 D-Glucose HK Assay Kit 货号:K-GLUHK-110A Megazyme中文站

D-葡萄糖[HK法]检测试剂盒

英文名:D-Glucose HK Assay Kit

货号:K-GLUHK-110A

规格:110 assays (manual) / 1100 (microplate) / 1000 (auto-analyser)

市场价: 2700

分析物意义:常见食品组分,在某些情况下非常重要,如糖尿病产品   

Megazyme检测试剂盒优点:选择简单可用的方法,葡萄糖氧化酶/过氧化酶 /己糖激酶/6-磷酸葡萄糖脱氢酶。试剂稳定

High purity reagents for the assay of D-glucose in plant and food products. Can be used in combination with other Megazyme products that require glucose determination. Content:110 assays per kit

UV-method for the determination of D-Glucose in foodstuffs,
beverages and other materials

Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size: (K-GLUHKR)
110 assays (manual) / 1100 (microplate)
/ 1000 (auto-analyser) or
(K-GLUHKL)
220 assays (manual) / 2200 (microplate)
/ 2000 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.66 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery
products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals
(e.g. infusions), feed, paper (and cardboard) and other materials (e.g.
biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. Is it possible to detect glucose when it is bound via a glycosidic linkage?

No.  The K-GLUHKL test kit is specific for the measurement of “free” D-glucose.  It will not detect glucose that is bound by a glycosidic linkage to another sugar molecule. 

Q5. Can K-GLUHKL be used to measure glucose in biological samples?

Yes.  It is possible that biological samples may be used directly after appropriate sample dilution in distilled water, however some biological samples may require deproteinisation with perchloric acid prior to addition to the assay.  The deproteinisation method can be found at the following link on the Megazyme website: Deproteinisation Method

Dilution during sample preparation must be taken into account in the final calculation.

Q6. Can K-GLUHKL be used to measure glucose as a component of polysaccharides in plant material?

Yes.  Determination of D-glucose in polysaccharides and fibrous plant material: 
Mill plant material or polysaccharide to pass a 0.5 mm screen using a Retsch centrifugal mill, or similar.  Accurately weigh approx. 100 mg of material into a Corning screw-cap culture tube (16 x 125 mm).  Add 5 mL of 1.3 M HCl to each tube and cap the tubes.  Incubate the tubes at 100˚C for 1 h.  Stir the tubes intermittently during the incubation.  Cool the tubes to room temperature, carefully loosen the caps and add 5 mL of 1.3 M NaOH.  Quantitatively transfer the contents of the tube to a 100 mL volumetric flask using distilled water and adjust the volume to 100 mL with distilled water.  Mix thoroughly by inversion and filter an aliquot of the solution through Whatman No. 1 filter paper or centrifuge at 1,500 g for 10 min.  Typically, no further dilution is required and a sample volume of 0.1 mL is satisfactory. 

Q7. Can oligosaccharides or polysaccharides be measured using the kit assay?

The kit assay will only measure the non-covalently linked monosaccharide.

Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.

Q8. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

“Hydrosphere” C18 色谱柱

“Hydrosphere”C18色谱柱

“Hydrosphere”C18色谱柱基体材料是一种高惰性超纯的PH中性的硅胶。”亲水C18″表面提高了极性物质的选择性,十分严格的物理和化学规范,适合通常的反相体系和高含量水的应用。

在药物分析需要LC-MS联用时,使用该柱则可不需要离子对试剂和缓冲液体系。

“Hydrosphere”C18是YMC在近20年生产的创新的反相物质的最新成员。它结合了超纯硅胶和均匀的选择性的特点,固定相充分实现了 HPLC 使用者的需要。最小的金属含量和低的硅醇基含量促使在分析碱性药物和螯合化合物时,在用 LC-MS 情况下也可在非磷酸盐缓冲液体系下使用。此外,分离极性化合物时在高水流动相下使用,不会使保留时间改变塔板数和峰型改变。这些性能使”Hydrosphere”C18 在能在有极性基团的化合物范围中得到广泛应用,所有柱都按严格的标准提供两种检测报告,填料和装填的色谱柱。

特别的色谱行为

在分离极性化合物时,在高水流动相的情况下,一般的反相柱将失去重现性。专利的衍生过程使”Hydrosphere”C18能被水穿透而不损失C18刷链。这种行为使其,将极性非常强的一组化合物互相分离开,并可以得到优良的峰型和重现性。

典型的利用是不需要有机改性剂可将核酸核苷酸有机酸茶多酚胺和维他命等分开。

规范

平均颗粒尺寸

4.5-5μ

平均孔尺寸

12-13nm(120-130A)

比表面积(m2/g)

320-350

孔体积(ml/g)

1.03-1.09

原子发射光谱)

Al

<10

Fe

<10

Na

<10

Ti

<0.5

碳含量(%)

11.5-13.0

典型应用

“Hydrosphere” C18 色谱柱

1.Tartaric acid
2.L-Malic acid
3.Lactic acid
4.Acetic acid
5.Citric acid
6.Succinic acid

A001218A

Column:Hydrosphere C18(5μm,12nm)

150X4.6mmI.D.

Eluent:20mM NaH2PO4-H3PO4(pH2.8)

Flow rate:1.0mL/min

Temperature:30

Detection:UV at 220nm,0.064AUFS

Injection:10μL(0.25-2.0mg/mL)

Organic acids

产品规格:

分析色谱柱

颗粒径
(μm)

微孔径

色谱柱尺寸
内径×长度
(mm)

产品型号

旧产品型号

S-3

12nm

1.0 X 35

HS12S03-H501WT

HS-3AH-3

1.0 X 50

HS12S03-0501WT

HS-3A0-3

1.0 X 100

HS12S03-1001WT

HS-3A1-3

1.5 X 35

HS12S03-H5P5WT

HS-3BH-3

1.5 X 50

HS12S03-05P5WT

HS-3B0-3

1.5 X 100

HS12S03-10P5WT

HS-3B1-3

2.0 X 35

HS12S03-H502WT

HS-3CH-3

2.0 X 50

HS12S03-0502WT

HS-3C0-3

2.0 X 75

HS12S03-L502WT

HS-3C7-3

2.0 X 100

HS12S03-1002WT

HS-3C1-3

2.0 X 150

HS12S03-1502WT

HS-3C2-3

3.0 X 50

HS12S03-0503WT

HS-3E0-3

3.0 X 100

HS12S03-1003WT

HS-3E1-3

3.0 X 150

HS12S03-1503WT

HS-3E2-3

4.6 X 35

HS12S03-H546WT

HS-30H-3

4.6 X 50

HS12S03-0546WT

HS-300-3

4.6 X 75

HS12S03-L546WT

HS-307-3

4.6 X 100

HS12S03-1046WT

HS-301-3

4.6 X 150

HS12S03-1546WT

HS-302-3

6.0 X 50

HS12S03-0506WT

HS-310-3

6.0 X 75

HS12S03-L506WT

HS-317-3

6.0 X 100

HS12S03-1006WT

HS-311-3

S-5

12nm

1.0 X 150

HS12S05-1501WT

HS-3A2

1.0 X 250

HS12S05-2501WT

HS-3A3

1.5 X 150

HS12S05-15P5WT

HS-3B2

1.5 X 250

HS12S05-25P5WT

HS-3B3

2.0 X 35

HS12S05-H502WT

HS-3CH

2.0 X 50

HS12S05-0502WT

HS-3C0

2.0 X 75

HS12S05-L502WT

HS-3C7

2.0 X 100

HS12S05-1002WT

HS-3C1

2.0 X 150

HS12S05-1502WT

HS-3C2

2.0 X 250

HS12S05-2502WT

HS-3C3

3.0 X 50

HS12S05-0503WT

HS-3E0

3.0 X 75

HS12S05-L503WT

HS-3E7

3.0 X 100

HS12S05-1003WT

HS-3E1

3.0 X 150

HS12S05-1503WT

HS-3E2

3.0 X 250

HS12S05-2503WT

HS-3E3

4.6 X 50

HS12S05-0546WT

HS-300

4.6 X 75

HS12S05-L546WT

HS-307

4.6 X 100

HS12S05-1046WT

HS-301

4.6 X 150

HS12S05-1546WT

HS-302

4.6 X 250

HS12S05-2546WT

HS-303

6.0 X 150

HS12S05-1506WT

HS-312

6.0 X 250

HS12S05-2506WT

HS-313

半制备色谱柱

颗粒径
(μm)

微孔径

色谱柱尺寸
内径×长度
(mm)

产品型号

旧产品型号

S-5

12nm

10 X 150

HS12S05-1510WT

HS-322

10 X 250

HS12S05-2510WT

HS-323

20 X 100

HS12S05-1020WT

20 X 150

HS12S05-1520WT

HS-342

20 X 250

HS12S05-2520WT

HS-343

0.53mm内径填充柱

0.53mm内径填充柱

内径0.53mm

填料种类广泛

对挥发性化合物有很好的保留和容量

适用于市场上的GC系统

                                   

货号

产品名称

长度

目数

温度上限

包装量

19041

Molesieve 5A

2m

80/100

300°C

ea.

19042

HayeSep Q

2m

80/100

275°C

ea.

19043

ShinCarbon ST

2m

80/100

330°C

ea.

19044

Rt-XLSulfur

2m

100/120

300°C

ea.

19045

ShinCarbon ST

1m

80/100

330°C

ea.

63,64-D-半乳-甘露戊糖 63,64-A-D- Galactosyl-Mannopentaose 货号:O-GGM5 Megazyme中文站

63,64-D-半乳-甘露戊糖

英文名:63,64-A-D- Galactosyl-Mannopentaose

货号:O-GGM5

规格:50 mg

市场价: 2800

CAS: 86781-72-8
Molecular Formula: C42H72O36
Molecular Weight: 1153.0
Purity: > 95%

High purity 63, 64-alpha-D-Galactosyl-mannopentaose for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

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TSKgel UP-SW3000分析柱

TSKgel UP-SW3000

➣ TSKgel UP-SW3000色谱柱是一款针对生物大分子开发的SEC色谱柱。

➣ 采用2um的硅胶作为基质,并用二醇基进行表面修饰。

➣ 填料孔隙性质被优化到校准曲线斜率较为平缓的程度,使得对于单抗二聚体/单体/片段具有很高的分离度。

➣ 填料表面性质与现有的SW系列色谱柱产品几乎相同。

➣ 2种色谱柱尺寸可供选择,

➣ 4.6 mm I.D. x 15 cm 的规格可用于超高速的分离。

➣ 4.6 mmI.D. x 30 cm 的规格可用于超高分辨率的分析。

➣ 可同时兼容于UHPLC和HPLC系统

➣ 必须使用半微型液相系统,即,较小死体积的液相系统才能实现较为理想的色谱柱性能。

产品规格

分析柱:

货号

产品名

粒径(um)

色谱柱尺寸(mm I.D. x cm)

排阻极限(kDa)*

0023449

TSKgelUP-SW3000

2

4.6 x 15

800

0023448

TSKgelUP-SW3000

2

4.6 x 30

800

保护柱:

货号

产品名

色谱柱尺寸(mm I.D. x cm)

备注

0023451

TSKgelguardcolumnUP-SW DC

4.6 x 2

出口端配有连接头

0023450

TSKgel guardcolumn UP-SW

4.6 x 2

 

强惰性保护柱和富集柱

强惰性保护柱和富集柱

聚乙烯乙二醇去活化层

当使用极性溶剂比如甲醇或水时,可以消除峰裂分

与Stabilwax,Rtx-225及Rt-2330毛细管柱兼容

可耐受操作温度:280°C

货号

内径

外径

长度

包装量

10065

0.25mm

0.37 ± 0.04mm

5m

ea.

10068

0.25mm

0.37 ± 0.04mm

10m

ea.

10014

0.25mm

0.37 ± 0.04mm

30m

ea.

10015

0.25mm

0.37 ± 0.04mm

60m

ea.

10066

0.32mm

0.45 ± 0.04mm

5m

ea.

10069

0.32mm

0.45 ± 0.04mm

10m

ea.

10024

0.32mm

0.45 ± 0.04mm

30m

ea.

10025

0.32mm

0.45 ± 0.04mm

60m

ea.

10067

0.53mm

0.69 ± 0.05mm

5m

ea.

10070

0.53mm

0.69 ± 0.05mm

10m

ea.

10034

0.53mm

0.69 ± 0.05mm

30m

ea.

10035

0.53mm

0.69 ± 0.05mm

60m

ea.

表位标记抗体 : Epitope Tag Antibody

表位标记抗体 : Epitope Tag Antibody

半井的抗体大多是小鼠和大鼠特异性单抗,用于融合蛋白检测和纯化。

Anti-GFP (Rat IgG2a), Mono (GF090R)

克隆: GF090R

同种型: IgG2a (Rat)

产品形态: Liquid

免疫原 : His-GFP (full length) fusion protein

应用 : Immunohistochemstry 1:1000-1:2000

Western Blotting 1:1000-1:2000

ELISA1:2000-1:20000

表位标记抗体 : Epitope Tag Antibody

表位标记抗体 : Epitope Tag Antibody

Anti-GFP (Mouse IgG1-k), Mono (GF200)

克隆: GF200

同种型: IgG1-k (Mouse)

产品形态: Liquid

免疫原 : His-GFP (full length) fusion protein

应用 : Western Blotting 1:1000-1:2000

ELISA1:2000-1:20000

表位标记抗体 : Epitope Tag Antibody

表位标记抗体 : Epitope Tag Antibody

Anti-c-Myc (Mouse IgG1-k), Mono (MC045)

克隆: MC045

同种型: IgG1-k (Mouse)

产品形态: Liquid

免疫原 : c-Myc synthetic peptide [EQKLISEEDL]conjugated with KLH

应用 : Western Blotting 1:1000-1:2000

Immunoprecipitation 1:400-1:1000

Immunocytochemstry 1:400-1:1000

ELISA1:2000-1:20000

表位标记抗体 : Epitope Tag Antibody

Anti-6xHis (Mouse IgG1a-k), Mono (HI192)

克隆: HI192

同种型: IgG1-k (Mouse)

产品形态: Liquid

免疫原 : 6xHis synthetic peptide [HHHHHH]conjugated with KLH

应用 : Western Blotting 1:1000-1:2000

ELISA1:2000-1:20000

表位标记抗体 : Epitope Tag Antibody

Anti-GST (Mouse IgG2a-k), Mono (GS019)

克隆: GS019

同种型: IgG2a-k (Mouse)

产品形态: Liquid

免疫原 : Gulutathione-S-Transferase (GST)

应用 : Western Blotting 1:1000-1:2000

Immunoprecipitation 1:400-1:1000

ELISA1:2000-1:20000

表位标记抗体 : Epitope Tag Antibody

Anti-V5 (Mouse IgG1-k), Mono (V5005)

克隆: V5005

同种型: IgG1-k (Mouse)

产品形态: Liquid

免疫原 : V5 synthetic peptide [GKPIPNPLLGLDST] conjugated with KLH

应用 : Western Blotting 1:1000-1:2000

Immunoprecipitation 1:400-1:1000

ELISA1:2000-1:20000

表位标记抗体 : Epitope Tag Antibody

Anti-HA (Mouse IgG1-k), Mono (HA124)

克隆: HA124

同种型: IgG1-k (Mouse)

产品形态: Liquid

免疫原 : Gulutathione-S-Transferase (GST)-HA[YPYDVPDYA-COOH] fusion protein

应用 : Western Blotting 1:10000-1:30000

ELISA 1:2000-1:20000

表位标记抗体 : Epitope Tag Antibody

表位标记抗体 : Epitope Tag Antibody

Anti-DYKDDDDK(Mouse IgG2b-κ), Mono

克隆: 5A8E5

同种型: IgG2b-κ (Mouse)

产品形态: Lyophilized form

特异性 : Anti-DYKDDDDK recognizes C-terminal, N-terminaland internal tagged fusion proteins.

浓度 : 0.5 mg/ml, lyophilized with PBS, pH7.4, containing0.02% sodium azide.

免疫原 : A synthetic peptide (DYKDDDDK) coupled KLH

应用 : Western Blotting 0.1-1.0 μg/ml

Immunoprecipitation 1 μg/ml

Immunofluorescent 1 μg/ml

ELISA0.05-0.2 μg/ml

表位标记抗体 : Epitope Tag Antibody

表位标记抗体 : Epitope Tag Antibody

订购信息

产品名称

应用

储存条件

货号

包装

Anti-c-Myc (Mouse IgG1-k), Monoclonal (MC045)

WB, IP, ICC, ELISA

4℃

04362-76
04362-34

50 ug
200ug

Anti-GFP (Mouse IgG1-k), Monoclonal (GF200)

WB,ELISA

4℃

04363-66
04363-24

50 ug
200ug

Anti-GFP (Rat IgG2a), Monoclonal (GF090R)

WB, IHC,ELISA

4℃

04404-26
04404-84

50 ug
200ug

Anti-GST (Mouse IgG2a-k), Monoclonal (GS019)

WB, IP,ELISA

4℃

04435-84
04435-26

50 ug
200ug

Anti-HA (Mouse IgG1-k), Monoclonal (HA124)

WB, ELISA

4℃

06340-96
06340-54

50 ug
200ug

Anti-6xHis (Mouse IgG1-k), Monoclonal (HI192)

WB, ELISA

4℃

04428-26
04428-84

50 ug
200ug

Anti-V5 (Mouse IgG1-k), Monoclonal (V5005)

WB, IP,ELISA

4℃

04434-94
04434-36

50 ug
200ug

Anti-DYKDDDDK (Mouse IgG2b-κ), Mono

WB, IP,IF, ELISA

-20℃

NU01102

200ug

Megazyme Incubation Bath MK III Megazyme Incubation Bath MK III 货号:D-IBMKIII Megazyme中文站

Megazyme Incubation Bath MK III

英文名:Megazyme Incubation Bath MK III

货号:D-IBMKIII

规格:Equipment as list in the Data Booklet

市场价: 7800

This bath is supplied with test-tube rack and 16 stirrer bars and tubes. With this bath and attachments, it is possible to stir 16 individual tubes and incubate at temperatures up to 60oC (+0.1oC). The magnetic stirrer and immersion heater must be purchased separately.

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HC-75 阳离子交换色谱柱

HC-75 阳离子交换色谱柱

HC-75阳离子交换色谱柱用于阳离子配位交换分离糖类。填料为磺酸化的苯乙烯-聚乙烯苯,粒径9μm ,柱子内径有4.1、7.8和10.0mm三种规格。316不锈钢柱体。有钙型(Ca),氢型(H)和铅型(Lead)三种。

➣ 水做流动相

➣ UV和RI检测

➣ 氢型色谱柱符合美国药典USP L17,钙型色谱柱符合美国药典USP L19,铅型色谱柱符合美国药典USP L34。

订货信息

9μm

9μm

9μm

I.D.×Length

钙型

铅型

氢型

4.1×250mm

79431

79476

7.8×100mm

79547

7.8×305mm

79436

79438

79544

10.0×250mm

79528

InertSustain C18

InertSustain C18

InertSustain C18 不仅沿承了Inertsil 系列液相色谱柱的优势(极低的柱背压、对许多分析物质具有高惰性、高柱效,而且与很多溶剂有着很好的兼容性),还能在宽PH范围情况下分析,并且不同批次的色谱柱之间具有稳定的柱效

高惰性

InertSustain C18的硅胶表表采用了新型去活化技术,并且残留硅烷醇的端基封尾化。吸附和峰拖尾减少到最小化。InertSustain C18 的高毒性可用于多种化合物,并得到尖锐的峰形。

碱性化合物

如下图显示,即使是强碱性化合物,如盐酸小檗碱(样品4)和右美沙芬(样品5),InertSustain C18也能提供高度稳定的色谱图,用于定性和定量分析。

InertSustain C18

酸性化合物

如下图所示,InertSustain C18具有中性硅胶表面,可提供出色的峰形。

InertSustain C18

螯合化合物

螯合化合物(如辛诺基替醇)通过与吸附剂表面的金属杂质结合而被吸附,从而引起严重的拖尾。由于金属会被注入的样品掩盖,这种吸附作用会随着注入的多次重复而消失。新开发的InertSustain C18硅胶几乎不含金属杂质。因此,它能够从一次注射中洗脱具有尖峰的螯合化合物。

InertSustain C18

· 在广泛的pH范围内具有高耐久性

Inertususlain C18对酸性和碱性流动相具有很高的耐受性,并且能够承受1到10的pH值。实验结果表明,在碱性条件下,Inertususlain C18保持了300小时的高效峰形,而其他品牌的”宽pH柱”则未能达到这种效果。

InertSustain C18

· 可靠稳定的重现性和性能

InertSustain C18具有出色的重现性。 它是在严格的物理质量控制下生产的,并经过严格的惰性测试以及选择性测试。

InertSustain C18

· 低背压

新型硅胶使得InertSustain C18能够在不牺牲效率的情况下以显著更低的背压进行操作。如下图所示,在市场上的许多C18柱中,InertSustainC18在低背压操作上是出类拔萃的。

InertSustain C18

规格

InertSustain C18

亲和层析填料

亲和层析填料

一、 金属螯合介质

可反复螯合不同金属,在用以结合依赖不同金属的蛋白。螯合蛋白透过外露的组氨酸,半胱氨酸和色氨酸等残基与不同金属作用。一种介质可做多种纯化工作。

二、 活化偶联介质

不同的配体可透过不同的化学基及目标配体,偶联至介质上。用来纯化与配体结合的生物分子。

三、 小配体亲和介质

四、 抗体亲和介质

重组蛋白A(rProteion A)的单抗载量高,基团脱落少。蛋白A比蛋白G专一,蛋白G能结合更多不同源的lgG

五、 颜料亲和介质

六、 外源凝集素亲和介质

七、 核酸(DNA & nucleotide)亲和介质

L-谷氨酸/氨检测试剂盒[快速] L-Glutamine/Ammonia (Rapid) Assay Kit 货号:K-GLNAM Megazyme中文站

L-谷氨酸/氨检测试剂盒[快速]

英文名:L-Glutamine/Ammonia (Rapid) Assay Kit

货号:K-GLNAM

规格:100 assays – 50 of each component

市场价: 3400

The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

UV-method for the determination of L-Glutamine and Ammonia
in cell culture media, foodstuffs and other materials

Principle:
(glutaminase)
(1) L-Glutamine + H2O → L-glutamate + NH4+

(microbial glutamate dehydrogenase)
(2) NH4+ + 2-Oxoglutarate + NADPH → L-glutamate + NADP+ + H2O

Kit size: 50 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 0.54 mg/L (L-glutamine)
0.06 mg/L (ammonia)
Application examples:
Cell culture media and cultures, dietary supplements, vegetables
and other materials (e.g. biological samples, etc.)
Method recognition: Novel method

Advantages

  • Very rapid reaction due to use of high activity glutaminase and uninhibited glutamate dehydrogense
  • All enzymes supplied as stabilised suspensions
  • Only enzymatic kit available
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

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D-葡萄糖[GOPOD法]检测试剂盒 D-Glucose (GOPOD Format) Assay Kit 货号:K-GLUC Megazyme中文站

D-葡萄糖[GOPOD法]检测试剂盒

英文名:D-Glucose (GOPOD Format) Assay Kit

货号:K-GLUC

规格:660 assays per kit

市场价: 3500

 分析物意义:常见食品组分,在某些情况下非常重要,如糖尿病产品   D-葡萄糖[GOPOD法]检测试剂盒 D-Glucose (GOPOD Format) Assay Kit 货号:K-GLUC  Megazyme中文站

Megazyme检测试剂盒优点:选择简单可用的方法,葡萄糖氧化酶/过氧化酶 /己糖激酶/6-磷酸葡萄糖脱氢酶。试剂稳定

D-葡萄糖[GOPOD法]检测试剂盒 D-Glucose (GOPOD Format) Assay Kit 货号:K-GLUC  Megazyme中文站

The D-Glucose test kit contains high purity reagents for the measurement and analysis of D-glucose in cereal extracts and for use in combination with other Megazyme kits.

Colourimetric method for the determination of D-Glucose in
foodstuffs, beverages and other materials

Principle:
(glucose oxidase)
(1) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(2) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 660 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 20 min
Detection limit: 100 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery
products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals,
feed, paper and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Widely used and accepted in clinical chemistry and food analysis

Advantages

  • All reagents stable for > 12 months after preparation
  • Very competitive price (cost per test)
  • Simple format
  • Standard included

 Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q2. I have some questions regarding the Glucose Oxidase and peroxidase in the Glucose Assay Kit. At what optimal pH do the enzymes work? Does the sample that is added to the enzyme mix show incorrect results if it is outside a certain pH range?

The test is best run at pH 7.4.  The reagent is buffered at this pH.  Using different pH values will affect results, i.e. it may take longer to reach this end point; but the same end-point value should be obtained if not too far away from this pH value.

Q3. What is the linear range of the Glucose Assay Kit?

The test is set up to measure between 10 and 100 micrograms per assay (i.e. 0.1 mL of 0.1 to 1.0 mg/mL).  You may prefer to use 3.0 mL of GOPOD reagent mixture plus 1.0 mL of sample; in this case the concentration range in the material being analysed (diluted) should be ~10 to 100 micrograms per mL.  The test is linear up to an absorbance of 1.4 (final assay volume of 3.2 mL).  If the final volume is 4.0 mL, then linearity will be up to about 1.0 absorbance units.

Q4. I have some questions on your Glucose Assay Kit. Is the colour stable at room temperature? Does the reaction continue if not read within 20 minutes?

The reaction is complete after approx. 15 min, and the colour is stable at temperatures of 20-50˚C for at least an extra hour.

Q5. Please let us know the lower limit of detection of measuring glucose using your Glucose Assay Kit (mg/mL).

1 microgram gives an absorbance of 0.01 if 3 mL of GOPOD are used.  If 1 mL of GOPOD is used, 1 microgram gives an absorbance of 0.03 OD. 

Q6. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q7. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q8. We stored the Glucose Assay Kit at 0-5°C for some weeks, because that is what is written on the package. Now we have noticed on some of the vials it says storage at –20°C. Can you tell me how long the products can be stored at 0-5°C without damage?

The kit enzymes can be stored at 0-5°C for up to 12 months, so they will be perfectly fine.

Q9. The temperature for the Glucose Assay Procedure (40 or 50˚C)-is this for incubation of the mix or reading the results at the spectrophotometer? Could I read the results at room temperature?

The temperature is just for the incubations.  It is an end-point assay, so the spectrophotometer does not need to be temperature controlled.

Q10. Can oligosaccharides or polysaccharides be measured using the kit assay?

The kit assay will only measure the non-covalently linked monosaccharide.

Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.

Rt-Alumina BOND/KCl

Rt-Alumina BOND/KCl

具有很好的重现性

可耐受的程序操作温度为200°C。更高的加热温度会导致多孔层的吸附特性发生不可逆的变化

质量控制方案:烃类测试混合物,测试色谱柱具有合适的固定相厚度和选择性;1,3-丁二烯,计算色谱柱的容量因子k,容量因子是固定相厚度的衡量工具;丙二烯和甲基乙炔的保留指数,衡量选择性;反式-2-丁烯和1-丁烯的分离度,衡量色谱柱的分离能力;1,3-丁二烯,测试色谱柱的塔板数

                                   

货号

长度

内径

膜厚

包装量

19776

30m

0.25mm

4μm

ea.

19761

30m

0.32mm

5μm

ea.

19762

50m

0.32mm

5μm

ea.

19759

30m

0.53mm

10μm

ea.

19760

50m

0.53mm

10μm

ea.

淀粉葡萄糖苷酶 Amyloglucosidase (Aspergillus niger) 货号:E-AMGFR-100MG Megazyme中文站

淀粉葡萄糖苷酶

英文名:Amyloglucosidase (Aspergillus niger)

货号:E-AMGFR-100MG

规格:100mg

市场价: 2800

High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3 
CAZy Family: GH15

From Aspergillus niger. Lyophilised powder. Electrophoretically homogenous. 

High purity. Recommended for use in AOAC Method 997.08 (fructan). 

Specific activity: ~ 35 U/mg (40oC, pH 4.5, soluble starch as substrate).

Stable at -20oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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甘露醇脱氢酶[荧光假单胞菌] Mannitol dehydrogenase (Pseudomona fluorescens) 货号:E-MNHPF Megazyme中文站

甘露醇脱氢酶[荧光假单胞菌]

英文名:Mannitol dehydrogenase (Pseudomona fluorescens)

货号:E-MNHPF

规格:500 Units

市场价: 2500

High purity recombinant Mannitol dehydrogenase (P. fluorescens) for use in research, biochemical enzyme assays andin vitro diagnostic analysis.

EC 1.1.1.67 

From Pseudomonas fluorescens. This recombinant enzyme has been expressed in E. coli and purified by affinity chromatography. Electrophoretically homogenous. 
In 3.2 M ammonium sulphate.

Specific activity: 48.8 U/mg protein on D-mannitol (pH 8.6, 40oC). Low Km, suitable for D-mannitol determination. 

Stable at 4oC for > 4 years.

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