Alpha葡[萄]糖苷酶 [转葡糖苷酶][黑曲霉] Alpha-Glucosidase(Transglucosidase) 货号:E-TRNGL Megazyme中文站

Alpha葡[萄]糖苷酶 [转葡糖苷酶][黑曲霉]

英文名:Alpha-Glucosidase(Transglucosidase)

货号:E-TRNGL

规格:2000 Units

市场价: 2600

Alpha葡[萄]糖苷酶 [转葡糖苷酶][黑曲霉]

EC 3.2.1.20
CAZY Family: GH31
From A. niger. In 3.2 M ammonium sulphate.
Specific activity: 70 U/mg (40°C, pH 4.5, maltose as substrate). 2.5 U/mg (phenyl α-glucoside as substrate).
Contaminants: α-amylase < 0.001%; amyloglucosidase < 0.01%.
Stable at 4°C for > 4 years.

 

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YMC-Actus Column

YMC-Actus Column

YMC-Actus HPLC色谱柱系列在高通量条件下,具有显著的耐久性。

特点:

• 耐用性强,适用DAC柱机制

• 柱子寿命长

• 非常适合实验室规模的分离和纯化

• 具有优异的分辨率

• 高通量

性能比较

稳定性试验结果、跟踪测试每100runs连续梯度的色谱柱性能测试结果如上图显示。

由于在制备分离和纯化的DAC柱领域有多年的经验,以及 YMC 的装填方法, YMC-Actus 有着优良的分辨率和耐久性。

YMC-Actus系列填料的类型和特性

填料

粒径(μm)

孔径(Å)

碳含量(%)

pH 值

特性

Pro series ODS

Pro C18

5

120

16

2 ~ 8

技术
* 分离碱性化合物

Hydrosphere C18

5

120

12

2 ~ 8

* 分离亲水化合物
* 可 100 %水作流动相

ProC18 RS

5

80

22

1 ~ 10

* 高持久性的 ODS
* 分离碱性化合物

YMC-Pack ODS series

ODS-A

5

120

17

2 ~ 7.5

* 应用广泛

ODS-AQ

5

120

14

2 ~ 7.5

* 分离亲水化合物

Other than ODS

Pro C8

5

120

10

2 ~ 7.5

* 封端技术
* 分离碱性化合物

订货信息

填料

粒径(μm)

孔径 (Å)

柱尺寸 I.D. ×长(mm)

产品型号

Pro C18

5

120

20 × 50

AS12S05-0520WX

Hydrosphere C18

5

120

20 × 50

HS12S05-0520WX

Pro C18 RS

5

80

20 × 50

RS08S05-0520WX

ODA-A

5

120

20 × 50

AA12S05-0520WX

ODA-AQ

5

120

20 × 50

AQ12S05-0520WX

Pro C8

5

120

20 × 50

OS12S05-0520WX

ZORBAX Extend-C18柱

ZORBAX Extend-C18柱

Agilent ZORBAX Extend-C18是使用一种独特的双配位C18-C18的键合技术的硅胶基质的柱子 ,以 RX-SIL为基础的反相键合相可在高、中、低PH范围内提供出色的峰形、样品的分离度及长的柱效命。 对于在高 pH溶液中更稳定或溶解度更高的化合物,采用高pH分离是较好的选择。有些物质非常适合做高PH值时流动相的缓冲液例如三乙胺、吡咯烷、甘氨酸、硼酸盐、氢氧化铵等。用LC/MS分析肽和一些小分子量的化合物时,使用pH值高的氢氧化胺比使用低pH的TFA做流动相时的灵敏度高。Extend-C18柱在pH2-11.5的范围内都很稳定,对所有的化合物都有很好的峰形,Extend-C18柱也为在低pH条件下分离提供了另一种选择。 适用于从科研到常规质量检测的各种纯化分析个案,可进行高通量的分析,是蛋白质组学分析的理想选择,同时也适用于分析各种化合物,分析范围广阔。

在高 PH值,碱性抗组织胺类药物在Extend-c18柱上得到了合适的保留值,良好的分离度和出色的峰形。

pH7 30%20mM Na2HPO4 70%甲醇pH11 30%20mM TEA 70%甲醇

ZORBAX Extend-C18柱

时间(分) 时间(分)

色谱柱: ZORBAX Extend-C18

4.6x150mm,5μm (Aglient 部件号:773450-902)

流动相:见图

流速: 1.0ml/min

温度:室温

检测: 254nm

样品:

1.顺丁烯二酸     pka

2.莨菪胺       7.6

3.伪麻黄碱      9.8

4.多西拉敏      9.2

5.扑尔敏       9.1

6.曲普立定      6.5

7.苯海拉明      9.0

聚合物基体的色谱柱 Extend-C18,4.6x250mm,5μm

4.0x250mm, 5μm (Agilent部件号:770450-902)

流速: 0.5ml/min流速:1.0ml/min

压力: 142bar 压力:213bar

ZORBAX Extend-C18柱

以硅胶为基体的 Extend-C18柱与聚合物键合相柱相比,在高PH值时,柱效更高,峰形更好,分析速度更快.而且,由于聚合物色谱柱的压力极限为180bar,因此,它只能在较低的流速下使用。

流动相 :65%20mM TEA,PH11,35%MeOH

温度:室温

检测: UV 254 nm

样品: 1.吡哆醇,2.吡定,3.N-甲苯胺,4.普鲁卡因,5.N-乙酰普鲁卡因。

产品规格

Extend-C18 窄径快速分离柱,2.1x50mm, 3.5um

Extend-C18 快速分离柱,4.6x50mm, 3.5um

Extend-C18 分析柱,4.6x50mm, 5um

Extend-C18 窄径柱,2.1x50mm, 5um

Extend-C18 微径快速分离柱,1.0x30mm, 3.5um

Extend-C18 微径快速分离柱,1.0x150mm, 3.5um

Extend-C18 快速分离柱,4.6x150mm, 3.5um

Extend-C18 微径快速分离柱,1.0x50mm, 3.5um

Extend-C18 快速分离柱,4.6x75mm, 3.5um

Extend-C18 分析柱,250×4.6mm,5um

Extend-C18 分析柱,150×4.6mm,5um

Extend-C18 窄径柱,2.1x150mm, 5um

高压 ZORBAX保护柱接头盒

Extend-C18 保护柱,12.5×4.6mm,5um

Rt/MXT-Alumina BOND/MAPD

Rt/MXT-Alumina BOND/MAPD

柱管经过Siltek处理,具有很强的惰性,对样品零吸附,确保痕量分析定量的准确性

键和密度高,分型对称度很好,对分析物业有很好的分离度

可以升温到250°C,对色谱柱进行高温老化,去除杂质

MXT柱管,韧性很好,可以调节柱架到3.5英寸,适用于便携气相

适用于石化以及下游产品的分析,如乙烯、丙烯、丁烯、丁二烯的分离分析

                                         

货号

名称

长度

内径

膜厚

框架直径

包装量

19779

Rt-Alumina BOND/MAPD

30m

0.32mm

5μm

ea.

19780

Rt-Alumina BOND/MAPD

50m

0.32mm

5μm

ea.

19777

Rt-Alumina BOND/MAPD

30m

0.53mm

10μm

ea.

19778

Rt-Alumina BOND/MAPD

50m

0.53mm

10μm

ea.

79728

MXT-Alumina BOND/MAPD

30m

0.53mm

10μm

7英寸

ea.

79728-273

MXT-Alumina BOND/MAPD

30m

0.53mm

10μm

3.5英寸

ea.

CHIRALPAK AS-3/AS-H/AS手性柱

CHIRALPAK AS-3/AS-H/AS手性柱

硅胶表面涂敷直链淀粉-三[(S)-α-甲苯基氨基甲酸酯]

CHIRALPAK  AS-3/AS-H/AS手性柱

货号

手性柱商品名

用途

内径mm

长度mm

粒径μm

20511

CHIRALPAK®AS-3

保护柱芯(*3)

4.0

10

3

20522

CHIRALPAK®AS-3

分析柱

4.6

50

3

20523

CHIRALPAK®AS-3

分析柱

4.6

100

3

20524

CHIRALPAK®AS-3

分析柱

4.6

150

3

20525

CHIRALPAK®AS-3

分析柱

4.6

250

3

20594

CHIRALPAK®AS-3

微径柱

2.1

150

3

20595

CHIRALPAK®AS-3

微径柱

2.1

250

3

货号

手性柱商品名

用途

内径mm

长mm

粒径μm

203C4

CHIRALPAK AS-H

Microflow

0.3

150

5

20311

CHIRALPAK AS-H

保护柱柱芯(×3)

4.0

10

5

20324

CHIRALPAK AS-H

分析柱

4.6

150

5

20325

CHIRALPAK AS-H

分析柱

4.6

250

5

20394

CHIRALPAK AS-H

微径柱

2.0

150

5

20335

CHIRALPAK AS-H

半制备柱

10

250

5

20345

CHIRALPAK AS-H

半制备柱

20

250

5

20375

CHIRALPAK AS-H

半制备柱

30

250

5

20233

CHIRALPAK AS

SMB(8支/套)

10

100

20

20242

CHIRALPAK AS

制备柱保护柱

20

50

20

20022

CHIRALPAK AS

保护柱

4.6

50

10

20025

CHIRALPAK AS

分析柱

4.6

250

10

20035

CHIRALPAK AS

半制备柱

10

250

10

20045

CHIRALPAK AS

半制备柱

20

250

10

超临界流体色谱用手性柱

20423

CHIRALPAK AS-H

SFC分析柱

4.6

100

5

20435

CHIRALPAK AS-H

SFC半制备柱

10

250

5

20445

CHIRALPAK AS-H

SFC半制备柱

20

250

5

20475

CHIRALPAK AS-H

SFC半制备柱

30

250

5

20455

CHIRALPAK AS-H

SFC制备柱

50

250

5

Zymo Research

ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪90年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒,其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各顶级实验室的认可,产品以”快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。

一级氨基氮[NOPA]检测试剂盒 Primary Amino Nitrogen Assay Kit (PANOPA) Assay Kit 货号:K-PANOPA Megazyme中文站

一级氨基氮[NOPA]检测试剂盒

英文名:Primary Amino Nitrogen Assay Kit (PANOPA) Assay Kit

货号:K-PANOPA

规格:100 assays (manual) / 1000 assays (microplate)

市场价: 2300

The Primary Amino Nitrogen (PANOPA) Assay Kit is suitable for the measurement and analysis of primary amino nitrogen in grape juice/must and wine.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Primary Amino Nitrogen in
grape juice, must, wine and other materials

Principle:
(room temperature)
(1) Amino nitrogen + N-acetyl-L-cysteine + o-phthaldialdehyde →
isoindole derivative

Kit size: 100 assays (manual) / 1000 (microplate)
/ 1100 (autoanalyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 2.59 mg N/L
Application examples:
Grape juice, must, wine and other materials
Method recognition: Novel method

Advantages

  • Simple format (absorbances read at 340 nm)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

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原生质体˚F Protoplast F 货号:E-PROTOF Megazyme中文站

原生质体˚F

英文名:Protoplast F

货号:E-PROTOF

规格:2.5 mL

市场价: 2900

Protoplast F is a lysing enzyme mixture containing cell wall degrading enzymes, e.g. exo-1,3-β-glucanase and endo-1,3-β-glucanase, and is used for generating protoplasts of Aspergillus niger.

The enzyme mixture is supplied as a precipitate in 3.2 M ammonium sulphate. 

Stability: > 2 years 4oC.

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β-N-乙酰己糖胺酶(原核生物) β-N-Acetylhexosaminidase (prokaryote) 货号:E-BNAHP Megazyme中文站

β-N-乙酰己糖胺酶(原核生物)

英文名:β-N-Acetylhexosaminidase (prokaryote)

货号:E-BNAHP

规格:100 U at 40°C

市场价: 2800

High purity β-N-Acetylhexosaminidase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.52
CAZy Family: GH20
CAS: 9012-33-3

beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase

Recombinant. From a prokaryote.
In 3.2 M ammonium sulphate.

Specific activity: 
~ 260 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 4.0 and 40°C; 
~ 723 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 4.0 and 60°C.

Stability: > 2 years 4°C

DESCRIPTION

β-N-Acetylhexosaminidase (prokaryote)

EC 3.2.1.52
CAZy Family: GH20
CAS: 9012-33-3

Synonyms:
beta-N-acetyl-D-hexosaminide 
N-acetylhexosaminohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 2 years at 4oC.

Specific activity:
~ 260 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 4.0 and 40oC;
~ 723 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 4.0 and 60oC.

Unit definition:
One Unit of pyruvate kinase activity is defined as the amount of enzyme required to convert one µmole of phosphoenolpyruvate to pyruvate per min in Tris.HCl buffer (100 mM) at pH 7.2 at 37oC.

Specificity:
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-β-D-hexosaminides from glycoproteins and oligosaccharides.

Applications:
For use in glycobiology research.

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α-淀粉酶(解淀粉芽孢杆菌) α-Amylase (Bacillus amyloliquefaciens) 货号:E-BAASS Megazyme中文站

α-淀粉酶(解淀粉芽孢杆菌)

英文名:α-Amylase (Bacillus amyloliquefaciens)

货号:E-BAASS

规格:2900 Units

市场价: 2000

High purity α-Amylase (Bacillus amyloliquefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.1
CAZY Family: GH13
CAS: 9000-90-2

alpha-amylase; 4-alpha-D-glucan glucanohydrolase

From Bacillus amyloliquefaciens.
In 50% (v/v) glycerol plus 0.02% sodium azide.
Supplied at ~ 140 U/mL.

Specific activity: ~ 50 U/mg (40oC, pH 6.5 on Ceralpha Reagent).

Stability: > 4 years at -20oC.

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Inertsil ODS-3 色谱柱

Inertsil ODS-3 色谱柱

Inertsil ODS-3 的填料是在高纯度球状硅胶化学修饰具有通用性的十八烷基,实施了理想的端基封尾。与其他的ODS色谱柱相比,它提供了更强的疏水性相互作用,并保留了各种化合物。在市场上众多的ODS色谱柱之中,Inertsil ODS-3凭借其通用性自推出以来就广受好评。

具有强保留型的惰性ODS色谱柱

由于良好的键合技术和端基封尾,Inertsil ODS-3具有高惰性。Inertsil ODS-3无论是使用于碱性或者酸性化合物,都能得到良好的峰形。

Inertsil ODS-3 色谱柱

Inertsil ODS-3 色谱柱

高重现性和使用耐久性

硅基硅的合成过程受到严格控制。此外,通过专有的结合和端盖技术,确保了惰性和选择性。它是一个可靠的ODS柱,它提供了良好的再现性和长列的生命周期。硅胶合成的每一道工序都经过严格把控。此外,通过专有的键合技术和端基封尾,确保了高惰性和选择性。Inertsil ODS-3具有良好的重现性以及耐久性,是一款值得信赖的色谱柱。

规格

Inertsil ODS-3 色谱柱

YMC-Pack FA/CA色谱柱

YMC-Pack FA/CA

此款色谱柱包括适用于脂肪酸、羟基羧酸分离用的FA柱和双羧酸用的CA柱两类。通过对样品进行衍生化处理,实现上述各类脂肪酸类的快速分离。

特点:

需进行2-NPH(2-Nitrophenylhydrazine)衍生

可进行高感度、高选择性的定量分析

衍生操作简单方便

短时间内实现多种成分的分离

简便的衍生化方法:

YMC-Pack FA/CA色谱柱

麦芽淀粉酶检测试剂盒 Malt Amylase Assay Kit 货号:K-MALTA Megazyme中文站

麦芽淀粉酶检测试剂盒

英文名:Malt Amylase Assay Kit

货号:K-MALTA

规格:100 assays (50 of each) per kit

市场价: 4200

The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.

Colourimetric method for the determination of α-Amylase and
β-Amylase in cereal grains, malt, food, beverages and
fermentation products

Principle:
(1) α-Amylase is measured using the “Ceralpha” Method as used
in K-CERA

(2) β-Amylase is measured using the “Betamyl-3” Method as used
in K-BETA3

Kit size: 50 assays of each
Method: Spectrophotometric at 400 nm
Reaction time: ~ 20 min (Ceralpha Method)
~ 10 min (Betamyl-3 Method)
Detection limit: 0.05 U/mL
Application examples:
Cereal flours, malts, fermentation broths and other materials
Method recognition:
“Ceralpha” Method: AOAC (Method 2002.01), AACC (Method
22-02.01), ICC (Standard No. 303), RACI
(Standard Method) and CCFRA (Flour Testing
Working Group Method 0018)
“Betamyl-3” Method: RACI (Standard Method)

Advantages

  • Very cost effective
  • All reagents stable for > 2 years as supplied
  • Only enzymatic kit available (Beta-Amylase)
  • Very specific
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

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COSMOSIL 5PBB

COSMOSIL 5PBB

COSMOSIL 5PBB 填充柱以 5溴基苄基为固定相。特别适合制备分离富勒烯,以二硫化碳或 1,2 ,4-三氯苯做洗脱剂。富勒烯在这种溶剂中的溶解性比甲苯好,COSMOSIL 5PBB 可高效分离富勒烯、金属富勒烯和其他衍生物。

填料特性

COSMOSIL 5PBB

硅胶

高纯多孔球形硅胶

平均粒径

5μm

平均孔径

120 Å

比表面积

300m2/g

固定相

5 溴基苄基

封端

几乎完全封端

碳含量

8%

分离高富勒烯

COSMOSIL 5PBB

订货信息

产品名称

尺寸

货号

COSMOSIL ® 5PBB 保护柱

4.6mmI.D.x 10mm

37987-31

10mmI.D.x 20mm

37988-21

20mmI.D.x 50mm

34375-31

COSMOSIL ® 5PBB 填充柱

4.6mmI.D.x 150mm

34274-51

4.6mmI.D.x 250mm

37980-01

10mmI.D.x 250mm

37985-51

20mmI.D.x 250mm

37986-41

转化酶(粉末)[酵母] Invertase Powder 货号:E-INVPD-5G Megazyme中文站

转化酶(粉末)[酵母]

英文名:Invertase Powder

货号:E-INVPD-5G

规格:5 grams

市场价: 3800

High purity Invertase (powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.26

β-Fructofuranosidase. Sucrase. Saccharase.

Specific activity: 300 U/mg of solid; 900 U/mg protein.

Data booklets for each pack size are located in the Technical Resources tab.

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三菱化学液相色谱柱

三菱化学液相色谱柱

产品特点

➣ 卓越的性能,球型颗粒,颗粒尺寸分布很窄

➣ 优良的技术和严格的质量控制,持久高质量的产品

➣ 粒度范围宽广,从 4 μ m 到 300 μ m ,可用于装填分析柱和制备柱

➣ 超过 40 年的丰富工艺和经验积累

选择指南

MCI-GEL 系列色谱柱一览表

性样品

MW>2000

尺寸排除

CQP10CQP30

pH范围2-12

蛋白质 生物聚合物水 溶性聚合物

离子交换

ProtEX 系列 CQA CQK

pH范围2-12

蛋白质 生物聚合物

疏水

CQH

pH范围2-12

蛋白质 生物聚合物

反相

CHP2MG

pH范围2-12

蛋白质 生物聚合物

CHP5C CHP10MG

pH范围1-13

水溶性样品

MW<2000

尺寸排除

CK02AS CK02A CK04SS CK04S

pH范围6-7

多聚糖 多聚糖

CQP06

pH范围2-12

离子交换

CK10U

pH范围1-14

氨基酸

CDR10 CA08F

pH范围1-13

有机酸 糖类

CDR10

pH范围1-13

核酸

SCA04

pH范围3-7

阴离子

SCK01

pH范围1.5-12

阳离子

反相

CHP2MG

pH范围2-12

有机化合物

CHP5C CHP10MG

pH范围1-13

有机化合物

离子排除

CK08EH

pH范围1-7

有机酸

配位体交换

CK08E 系列 CRS10 ➣ CRS15W

pH范围5-7

糖类 光学异构体

有机溶剂可溶样品 MW<2000

反相

GHP 5C GHP10M GHP2MG

有机化合物 有机化合物

➣ 反相色谱 MCI-GEL CHP 系列——在药物纯化和分析中应用最广泛

其中包括 MCI-GEL CHP 中粒径和小粒径的填料和小粒径的高压液相色谱柱

柱子型号

柱尺寸 (mm)

填 料

应 用

交联度(%)

平衡离子

粒径(µm)

氨基酸

单糖小糖

寡糖

羧酸

生理液体

CK10U

6×120

10

Nal+

5

CK08S

8×500

8

Na+

11

CK08E

8×300

8

Na+

9

CK08EC

8×300

8

Ca+2+

9

CK08EP

8×300

8

Pb2+

9

CK08ES

8×300

8

Ag+

9

CK08EH

8×300

8

H+

9

CK04S

10×200

4

Na+

11

CK04SS

10×200

4

Ag+

11

CK02A

20×250

2

Na+

20

CK02AS

20×250

2

Ag+

20

CA08F

4.6×250

8

Cl-

7

CDR10

4.6×250

多孔

AcO-

7

MCI-GEL 各种离子交换色谱柱的性能和应用

为了使广大用户更熟悉三菱化学 MCI-GEL 的离子交换柱现将离子交换的代号描述如下

三菱化学液相色谱柱

用于氨基酸分析的有 CK10U 也就是 聚苯乙烯二乙烯基苯为基体阳离子交换 柱钠型5μ

用于糖、糖醇、酸等分离的阳离子交换

MCIGEL CK08S CK08E

Na+

普通的糖分离柱

MCIGEL CK08EC

Ca++

最常用分离糖的色谱柱常推荐用于分离葡萄糖和麦芽糖

MCIGEL CK08EP

Pb++

糖醇和糖

MCIGEL CK08ES

Ag+

凝胶过滤色谱

MCIGEL CK08EH

H+

用磷酸流动相分离酸用水分离糖

➣ MCI GEL CQH 疏水作用色谱柱系列

MCI GEL CQH 系列属于疏水作用色谱柱,填料的功能基团分别为醚、丁基及苯基。

相对疏水性按下列顺序递减:

CQH3PS>CQH3BS>CQH3ES

柱子型号

柱尺寸(mm)

粒径(μm)

功能基团

CQH3ES

7.5I.D. × 75

10

CQH3BS

7.5I.D. × 75

10

丁基

CQH3PS

7.5I.D. × 75

10

苯基

➣ MCI GEL®CQP 亲水性尺寸排除色谱柱

尺寸排除色谱是依据溶质分子在溶液中尺寸大小不同而进行分离的一种液相色谱技术。色谱柱以多孔粒子为填料,不同溶质由于在填料的孔内外分布不同而得以分离。溶质分子中大于填料孔径的将被排除,因而首先洗脱下来,滞留时间短。 CQP 系列柱以疏水性聚合物为填料,用以对水溶性聚合物进行分析,例如寡糖等。

柱子型号

柱尺寸(mm)

填料

理论塔板数

排除极限

粒径(µm)

孔径(A)

(TP/柱)

(PEG)

CQP06

7.5I.D.×600

10

120

10000

~1×10 3

CQP10

7.5I.D.×600

10

200

6000

~1×10 4

CQP30

7.5I.D.×600

10

600

6000

~1×10 6

➣ ProtEx 系列离子交换色谱柱甲基丙烯酸

ProtEx 系列柱是用于分离蛋白质样品的离子交换色谱柱,填料为 5µm 、多孔的聚合物。 ProtEx 系列柱可以减少常常发生于普通蛋白质分离柱上的蛋白质非特异性吸收,这是由于填料碱性材料和离子交换基团之间的疏水层大大增加所致。

柱子型号

柱尺寸(mm)

填料

pH 值范围

粒径(µm)

功能基团

ProtEx-DEAE

4.6 I.D.×50

5

二乙基氨基乙基

2 — 12

7.5 I.D.×100

5

二乙基氨基乙基

2 — 12

ProtEx-SP

4.6 I.D.×50

5

磺酸丙基

2 — 12

7.5 I.D.×100

5

磺酸丙基

2 — 12

可吸收碳水化合物检测试剂盒 Available Carbohydrates/Dietary Fiber Assay Kit 货号:K-ACHDF Megazyme中文站

可吸收碳水化合物检测试剂盒

英文名: Available Carbohydrates/Dietary Fiber Assay Kit

货号:K-ACHDF

规格:100 assays of each component

市场价: 5300

分析物意义:  快速消化和吸收的糖及膳食纤维

Megazyme检测试剂盒优点:检测程序新颖、试剂稳定 

An integrated procedure for the measurement of available carbohydrates and dietary fibre in cereal products, fruit and vegetables and food products. Content:100 assays of each component

An integrated procedure for the measurement of Available
Carbohydrates and Dietary Fiber in cereal products, fruit and
vegetables and food products

Principle (Dietary Fiber):
(α-amylase + amyloglucosidase)
(1) Starch + H2O → glucose

(protease)
(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following
alcohol precipitation


Principle (Available Carbohydrates):
(sucrase / maltase + β-galactosidase)
(4) Sucrose, maltose and lactose → D-glucose + D-fructose
+ D-galactose


(PGI, hexokinase + glucose-6-phosphate dehydrogenase)
(5) D-Glucose + D-fructose + ATP + NADP+ → gluconate-6-phosphate
+ NADPH + ADP + H+

Kit size: 100 assays of each
Application examples:
Food ingredients, food products and other materials
Method recognition:
Dietary Fibre - AOAC (Methods 985.29, 991.42, 991.43 and 993.19)
and AACC (Methods 32-05.01, 32-07.01 and 32-21.01)

Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • High purity / standardised enzymes employed
  • Only kit available
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Simple format

 Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Pinnacle II C8色谱柱

Pinnacle II C8

产品描述:

粒径(μm):3、5,球型

孔径(Å):110

碳载量(%):7

封尾:是

PH范围:2.5 – 8

温度上限(°C):80

货号

粒径

柱长

内径

包装量

9213331

3μm

30mm

1.0mm

ea.

9213351

3μm

50mm

1.0mm

ea.

9213311

3μm

100mm

1.0mm

ea.

9213361

3μm

150mm

1.0mm

ea.

9213332

3μm

30mm

2.1mm

ea.

9213352

3μm

50mm

2.1mm

ea.

9213312

3μm

100mm

2.1mm

ea.

9213362

3μm

150mm

2.1mm

ea.

9213333

3μm

30mm

3.2mm

ea.

9213353

3μm

50mm

3.2mm

ea.

9213313

3μm

100mm

3.2mm

ea.

9213363

3μm

150mm

3.2mm

ea.

9213335

3μm

30mm

4.6mm

ea.

9213355

3μm

50mm

4.6mm

ea.

9213315

3μm

100mm

4.6mm

ea.

9213365

3μm

150mm

4.6mm

ea.

9213531

5μm

30mm

1.0mm

ea.

9213551

5μm

50mm

1.0mm

ea.

9213511

5μm

100mm

1.0mm

ea.

9213561

5μm

150mm

1.0mm

ea.

9213521

5μm

200mm

1.0mm

ea.

9213571

5μm

250mm

1.0mm

ea.

9213532

5μm

30mm

2.1mm

ea.

9213552

5μm

50mm

2.1mm

ea.

9213512

5μm

100mm

2.1mm

ea.

9213562

5μm

150mm

2.1mm

ea.

9213522

5μm

200mm

2.1mm

ea.

9213572

5μm

250mm

2.1mm

ea.

9213533

5μm

30mm

3.2mm

ea.

9213553

5μm

50mm

3.2mm

ea.

9213513

5μm

100mm

3.2mm

ea.

9213563

5μm

150mm

3.2mm

ea.

9213523

5μm

200mm

3.2mm

ea.

9213573

5μm

250mm

3.2mm

ea.

9213514

5μm

100mm

4.0mm

ea.

9213564

5μm

150mm

4.0mm

ea.

9213535

5μm

30mm

4.6mm

ea.

9213555

5μm

50mm

4.6mm

ea.

9213515

5μm

100mm

4.6mm

ea.

9213565

5μm

150mm

4.6mm

ea.

9213525

5μm

200mm

4.6mm

ea.

9213575

5μm

250mm

4.6mm

ea.

TSKgel STAT系列离子交换色谱柱

TSKgel STAT系列离子交换色谱柱

产品简介:

TSKgel STAT系列为生物高分子(蛋白质,多肽,核酸)分离用聚合物系离子交换色谱柱。

使用非多孔性填料(NPR),在通用LC系统中可实现超高速?超高分离。

TSKgel STAT系列离子交换色谱柱

具有阴离子交换色谱柱(TSKgel Q-STAT)和阳离子交换色谱柱(TSKgel SP-STAT、TSKgel CM-STAT),根据样品可选择色谱柱。

具有核酸分离用阴离子交换色谱柱。(TSKgel DNA-STAT)

具有超高速分离用色谱柱(粒径10μm、3.0mmI.D.×3.5cm)和超高分离用色谱柱(粒径7μm、4.6mmI.D.×10cm),根据用途可选择色谱柱。(TSKgel DNA-STAT的粒径为5μm、4.6mmI.D.×10cm)。

用途

➣超高速分离用色谱柱 蛋白质的超高速分离(1分以内)

生物医药品候补蛋白质的筛选

实时监控PEG化蛋白质的PEG化反应

➣超高分离用色谱柱 单克隆抗体变异体的分离,质量管理

PEG化蛋白质的宁集体分离

生物医药品的质量管理

核酸分离用色谱柱 siRNA,寡核苷酸的分离

质粒DNA、巨大DNA片段的分离

核酸医药品德质量管理 

相关阅读:TSK-GEL H系列凝胶渗透柱

菊粉内切(黑曲霉)(重组) endo-Inulinase (Aspergillus niger) (Recombinant) 货号:E-ENDOIAN Megazyme中文站

菊粉内切(黑曲霉)(重组)

英文名:endo-Inulinase (Aspergillus niger) (Recombinant)

货号:E-ENDOIAN

规格:500 Units at 40°C

市场价: 2400

High purity recombinant endo-Inulinase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.7
CAZy Family: GH32
CAS:  9025-67-6

inulinase; 1-beta-D-fructan fructanohydrolase

Recombinant. From Aspergillus niger.
In 3.2 M ammonium sulphate.
Supplied at ~ 200 U/mL. 

Specific activity: ~ 580 U/mg (60oC, pH 4.5 on inulin); ~ 300 U/mg (40oC, pH 4.5 on inulin).

Stability: > 4 years at 4oC.

暂无问题解答

暂无视频

1,4-β-D-葡糖基-D-甘露二糖加1,4-β-D-纤维二糖苷-D-甘露糖 1,4-β-D-Glucosyl-D-Mannobiose plus 1,4-β-D-Cellobiosyl-D-Mannose 货号:O-GMMTR Megazyme中文站

1,4-β-D-葡糖基-D-甘露二糖加1,4-β-D-纤维二糖苷-D-甘露糖

英文名:1,4-β-D-Glucosyl-D-Mannobiose plus 1,4-β-D-Cellobiosyl-D-Mannose

货号:O-GMMTR

规格:50 mg

市场价: 2528

CAS: 28072-83-5
          28072-82-4
Molecular Formula: C18H32O16
Molecular Weight: 504.4
Purity: > 90%

High purity 1,4-β-D-Glucosyl-D-Mannobiose and 1,4-β-D-Cellobiosyl-D-Mannose for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

Trisaccharides from hyrolysis of glucomannan. 

暂无问题解答

暂无视频

综合总膳食纤维检测试剂盒 Integrated Total Dietary Fiber Assay Kit 货号:K-INTDF Megazyme中文站

综合总膳食纤维检测试剂盒

英文名:Integrated Total Dietary Fiber Assay Kit

货号:K-INTDF

规格:100 assays per kit

市场价: 4000

产品名称:综合总膳食纤维检测试剂盒
英文名称: Inegrated Total Dietary Fibre Assay Kit
型号规格:100次
品牌:爱尔兰Megaeyme公司

比重〜1.8
在水中的溶解度:易溶
pH值:7.0
气味:无
状态:液体
保质期:十年或以上(冰箱内)

成分
三玻璃瓶
名称   CAS
a-淀粉酶(胰)酶9014-71-5 1瓶(玻璃)
淀粉葡萄糖苷酶酶9032-08-0 1瓶(玻璃)
蛋白酶9014-01-1 1瓶(玻璃)

1. 简介:
膳食纤维是一种混合的复杂的有机物质,包括
亲水性化合物,如可溶性和不溶性多糖
和非消化寡糖以及一系列非
膨胀,或多或少疏水性化合物,如cutins ,
suberins和木质素。
膳食纤维是指碳水化合物及其相类似物质的总和,包括多糖、寡糖、木质素
以及相关的植物物质。 主要有纤维素、 半纤维素、 果胶及亲水胶体物质, 如树胶、
海藻多糖等组分;另外还包括植物细胞壁中所含有的木质素;不被人体消化酶所
分解的物质,如抗性淀粉、抗性糊精、抗性低聚糖、改性纤维素、黏质、寡糖以
及少量相关成分, 如蜡纸、 角质、 软木脂等。 总膳食纤维检测试剂盒是根据AOAC
991.43,AOAC 985.29,AACC32-07和AACC 32-05方法开发的,也适用于其它方
法,如AACC Method 32-21,AACC method32-06。
2. 检测原理
总膳食纤维(华盈)确定重复样品干
和脱脂(如脂肪含量是“ 10 % )的材料。样品在熟食
〜 100 ℃的热稳定α -淀粉酶,使gelatinisation ,水解
和depolymerisation的淀粉;孵育在60 ℃与蛋白酶(以
solubilise和depolymerise蛋白质)和淀粉葡萄糖苷酶(以
水解淀粉碎片葡萄糖) ;和治疗四卷
乙醇沉淀可溶性纤维和删除depolymerised
蛋白质和葡萄糖(淀粉) 。残渣过滤;洗
78 %乙醇, 95 %的乙醇和丙酮;干;和权衡。一个
重复分析蛋白质和其他的孵育在525摄氏度
确定灰。在华盈是重量过滤,干燥
残留物的重量少的蛋白质和灰烬。
主要利用Megazyme华盈试剂盒是,它包含
高纯度酶缺乏干预活动,活动
酵素是标准化。标准化的重要性
α -淀粉酶活性的测定抗性淀粉是良好
认可。 Megazyme淀粉葡萄糖苷酶基本上没有
纤维素酶,而其他常用的准备工作包含
重大污染这一活动,从而导致
solubilisation和低估了β -葡聚糖。所有Megazyme华盈
酶提供一个随时可以使用的,稳定,液态形式。
范围:
适用于谷物,水果和蔬菜,谷物和水果
产品和食品。
酶的纯度和标准化:
的有效性和纯洁性Megazyme α -淀粉酶,蛋白酶和
淀粉葡萄糖苷酶已经评价的标准
建议AOAC方法985.29和991.43 ,和油菜
方法32-05 。 Megazyme耐高温α -淀粉酶(电子BLAAM )有一个
活动3000 U / ml的( Ceralpha方法) ;蛋白酶是提供在
1concentration为50毫克/毫升( 〜 350酪氨酸单位/毫升) ;和
淀粉葡萄糖苷酶提供的浓度为200 U / ml的
(硝基苯β – maltoside基板) (或3300 U / ml的可溶性
淀粉) 。这种淀粉葡萄糖苷酶的活动是150 %的浓度
传统上用于华盈化验,以便0.2毫升(而不是0.3毫升)是
用于试验。
Megazyme淀粉葡萄糖苷酶(电子AMGDF )基本上是缺乏
纤维素酶,而在其他准备用于华盈决心,
纤维素酶污染可高达1 %
淀粉葡萄糖苷酶(对一项活动的基础上) 。这种程度的污染
导致低估的β -葡聚糖高达10-15 % 。
方法用于测量和标准化的Megazyme
酶,可以要求。

脱脂(如大于10%)干燥后的样品经热稳定a-淀粉酶、蛋白酶和淀粉葡萄糖
苷酶酶解消化, 酶解液通过乙醇沉淀、 过滤, 乙醇和丙酮洗涤残渣后干燥、 称重,
得到总膳食纤维(TDF)残渣;酶解液通过直接过滤、热水洗涤残渣,干燥后称
重,得到不溶性膳食纤维(IDF)残渣;滤液用乙醇沉淀,过滤、干燥、称重,
得到可溶性膳食纤维(SDF)残渣。TDF、IDF和SDF的残渣扣除蛋白质、灰分
和空白即得TDF、IDF和SDF含量。
Megazyme试剂盒的主要优势在于直接提供高纯度的酶,酶的活力是标准化
的,而标准化的a-淀粉酶的活力在测量抗性淀粉是公认的。Megazyme的淀粉葡
萄糖苷酶中基本没有纤维素酶,而其他常用的酶制剂中常含有这种污染物,这将
导致溶解和低估β-葡聚糖。Megazyme试剂盒提供的酶都是稳定的液体,可以保
存到试剂盒有效期,并且是直接应用液,不需要使用前再溶解。
3. 用途
适用于检测谷物、水果、蔬菜和加工食品中的总膳食纤维(TDF) 、不溶性
膳食纤维(IDF)和可溶性膳食纤维(SDF)的检测。 

The Integrated Total Dietary Fiber test kit is suitable for the measurement and analysis of Integrated Total Dietary Fiber.
Updated to include resistant, branched maltodextrins as a component in dietary fiber.
K-INTDF- An Integrated Procedure (AOAC Method 2009.01 & 2011.25) for the measurement of Total Dietary Fiber (including resistant starch and non-digestible oligosaccharides). This method combines the key attributes of AOAC Official Methods of Analysis 2002.02, 985.29, 991.43, 2001.03.
Specific dietary fiber fractions are measured as follows:
1. Insoluble dietary fiber (IDF), Higher Molecular Weight Soluble Dietary Fiber (SDFP) and Lower Molecular Weight Soluble Dietary Fiber (SDFS) determination (AOAC Method 2011.25)
2. Total High Molecular Weight Dietary Fiber (HMWDF) and SDFS determination (AOAC Method 2009.01)
The enzymes used in these methods are of very high purity; they are effectively devoid of contaminating enzymes active on β-glucan, pectin and arabinoxylan.
Data calculators are located in the Technical Resources tab.

For the determination of Total Dietary Fiber in cereal products, foodstuffs, feeds and other materials. CODEX Type I method (2011)

Principle:
(Pancreatic α-amylase + amyloglucosidase)
(1) Non-resistant starch + H2O → D-glucose

(protease)
(2) Protein + H2O → peptides

(3) IDF (including resistant starch) and alcohol precipitated
soluble DF (SDFP) determined gravimetrically

(4) Alcohol soluble DF (SDFS) determined by HPLC

(5) Ash and residual protein determined on DF residues
and subtracted

Kit size: 100 assays
Method: Hydrolysis / removal of non-dietary
fibre components
Total assay time: ~ 3 hr work (over 2 days)
Detection limit: 0.5-100% of sample weight
Application examples:
Food ingredients, food products and other materials
Method recognition:
AOAC (Methods 2009.01 and 2011.25), AACC (Method 32-45.01 and
32-50.01) and CODEX (Type I method)

Advantages

  • The only method that is consistent with the CODEX Alimentarius definition of dietary fibre
  • High purity / standardised enzymes employed
  • All reagents stable for > 2 years
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Very competitive price (cost per test)

 Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. Is there a preferred rate of heating and cooling for the recommended crucible (Corning® No. 32940-50C or equivalent) to avoid breakages?

The crucible has the potential to break if heated or cooled too rapidly.  The graph below shows the manufacturer’s recommended heating and cooling profile forsafe use with the crucible. 
综合总膳食纤维检测试剂盒 Integrated Total Dietary Fiber Assay Kit 货号:K-INTDF  Megazyme中文站
Application Note:
Removal of Sucrose, Maltose and Fructose from F3
SUCRASE (MALTASE) + β-GALACTOSIDASE (Lot 111101)
E-SUCRBG
Sucrase (170 U), α-glucosidase (2600 U) and β-galactosidase (3000 U); freeze dried.
For use in the removal of sucrose, maltose and lactose in dietary fibre determinations.  In the Integrated Total Dietary Fibre procedure, in HPLC analysis of non-digestible oligosaccharides using the Waters Sugar-Pak column, the fructosyl-trisaccharide  β-D-Fruf-(2→1)-β-D-Fruf-(2→1)-β-D-Fruf, chromatographs at a similar point to the disaccharides, sucrose, maltose and lactose.  Accurate determination of this trisaccharide requires the hydrolysis of these disaccharides.  This can be achieved using this enzyme mixture.
PROPERTIES
1. ELECTROPHORETIC PURITY:
This is a mixture of sucrase (maltase; from Bacillus stearothermophilis):
– Single major band on SDS-gel electrophoresis (57,750) plus β-galactosidase (from A. niger)
– Single band on isoelectric focusing
2. ACTIVITY:
This enzyme mixture gives complete hydrolysis of sucrose, maltose and lactose under the defined assay conditions, with no hydrolysis of the trisaccharide, β-D-Fruf-(2→1)-β-D-Fruf-(2→1)-β-D-Fruf.
3. STORAGE CONDITIONS:
The enzyme is supplied as a lyophilised powder and should be stored at -20˚C.  On dissolution in buffer or water, the enzyme should be stored in the frozen state.  It is recommended that all buffers used for dilution contain BSA (0.5 mg/mL).
4. PREPARATION OF ENZYME FOR USE:
Dissolve the contents of one vial in 6 mL of 5 mM sodium acetate buffer (pH 5.0).  Transfer aliquots of approx. 2 mL to polypropylene tubes and store at -20˚C between use.  Can be thawed and re-frozen several times.
INCUBATION CONDITIONS:
To 1 mL of sugar mixture obtained in the Integrated Total Dietary Fibre procedure [Step I(b)] containing up to 5 mg/mL of sucrose, maltose and/or lactose and fructotriose,
add:
0.1 mL of sucrase/β-galactosidase enzyme mixture, and incubate at 40˚C, for 60 min.  Terminate the reaction by incubating the tube at 100˚C for 2 min and centrifuge the suspension in a Microfuge at 12,000 rpm for 5 min.
SAMPLE PREPARATION AND HPLC:
Analyse the supernatant solution by HPLC using a Waters Sugar-Pak® column as described in the Megazyme kit data booklet for the Integrated Total Dietary Fibre method (K-INTDF).  Calculate the amount of fructo-triose by reference to the D-sorbitol internal standard.  This amount should then be added to the determined amount of non-digestible oligosaccharides [NDO; low molecular weight  soluble dietary fibre; Soluble Dietary Fibre soluble in 78% aqueous ethanol (SDFS)].
综合总膳食纤维检测试剂盒 Integrated Total Dietary Fiber Assay Kit 货号:K-INTDF  Megazyme中文站

Q4. How should I prepare the required 78% ethanol solution?

A volume reduction occurs on mixing water with 95% ethanol (or IMS). 1 L of 78% ethanol is prepared by adding 821 mL 95% ethanol (or IMS) to 207 mL H2O.
If using a 1 L volumetric flask, this is best accomplished by placing 821 mL 95% ethanol (or IMS) into the volumetric flask. Dilute to volume with deionised water. Mix well. Check the level and if necessary add more deionised water to bring it back up to the 1 L mark. 

Megazyme 溶解淀粉 操作视频

Megazyme 试剂盒样品前处理准备操作视频

Megazyme综合总膳食纤维检测试剂盒操作视频(K-INTDF)

乙醇脱氢酶[大肠杆菌] Alcohol dehydrogenase (E. coli) 货号:E-ADHEC Megazyme中文站

乙醇脱氢酶[大肠杆菌]

英文名:Alcohol dehydrogenase (E. coli)

货号:E-ADHEC

规格:1000 Units

市场价: 2500

High purity recombinant Alcohol dehydrogenase (E. coli) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 1.1.1.1

Recombinant from E. coli. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 10 U/mg (25oC, pH 8.5).

Low Km for ethanol: due to the low Km of this enzyme, only 3 units are required per assay (TV = 2.54 mL) compared to 177 units of the yeast enzyme.

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