TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX* 货号17277-AAT Bioquest荧光染料

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TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*    货号17277 货号 17277 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17277

产品名称:TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

规格:5ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

TAQuest FAST qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液,针对 qPCR 和两步法 RT-qPCR 进行了优化。对于 20 uL 反应体积中的 40 个 PCR 循环,预混液可在 50 分钟内提供结果。该混合物包括含有 dNTP 的优化缓冲液和我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶,该酶旨在允许即时热启动,最大限度地减少非特异性产物的形成,从而允许室温反应设置。运行所需的 PCR 反应只需要模板和目标引物。 TAQuest FAST qPCR Master Mix 与 Helixyte Green 确保 PCR 特异性和灵敏度对所有样品类型,如基因组、质粒、病毒和 cDNA 模板。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析 DNA。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*。 

 

适用仪器


qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *无ROX* 解冻 TAQuest™ FAST qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *无 ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Fatal systemic toxoplasmosis in a 3-month-old young tibetan goat (Capra hircus).
Authors: Pavone, Silvia and Crotti, Silvia and Cruciani, Deborah and D’Avino, Nicoletta and Zema, Jacopo and Morelli, Simone and Gobbi, Marco and Madeo, Laura
Journal: BMC veterinary research (2020): 423

Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus).
Authors: Kang, Tae Sun
Journal: Food chemistry (2019): 1-8

A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants.
Authors: Sebastiani, Carla and Curcio, Ludovica and Ciullo, Marcella and Cruciani, Deborah and Crotti, Silvia and Pesca, Cristina and Torricelli, Martina and Sebastianelli, Martina and Felici, Andrea and Biagetti, Massimo
Journal: Journal of microbiological methods (2018): 12-17

A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species.
Authors: Kim, Mi-Ra and Kwon, Kisung and Jung, Yoo-Kyung and Kang, Tae Sun
Journal: Food chemistry (2018): 112-119

Development of a Sensitive Real-Time Fast-qPCR Based on SYBR® Green for Detection and Quantification of Chicken Parvovirus (ChPV).
Authors: Nuñez, Luis F and Santander-Parra, Silvana H and Chaible, Lucas and De la Torre, David I and Buim, Marcos R and Murakami, Alexandre and Zaidan Dagli, Maria Lucia and Astolfi-Ferreira, Claudete S and Piantino Ferreira, Antonio J
Journal: Veterinary sciences (2018)

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Fast quantitative PCR, locked nucleic acid probes and reduced volume reactions are effective tools for detecting Batrachochytrium dendrobatidis DNA.
Authors: Ruthig, Gregory R and Deridder, Benjamin P
Journal: Diseases of aquatic organisms (2012): 249-53

Multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness.
Authors: Buzard, Gregory S and Baker, Daniel and Wolcott, Mark J and Norwood, David A and Dauphin, Leslie A
Journal: Forensic science international (2012): 292-7

Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles.
Authors: Yoder, Kristine E and Fishel, Richard
Journal: Journal of virological methods (2008): 253-6

Detection of equine herpesvirus-1 in nasal swabs of horses by quantitative real-time PCR.
Authors: Perkins, G A and Goodman, L B and Dubovi, E J and Kim, S G and Osterrieder, N
Journal: Journal of veterinary internal medicine: 1234-8