VECTOR 生物素苋尾状凝集素（ACL，ACA） BIOTIN-ACL

• 产品型号：  B-1255
• 简单描述
• VECTOR 生物素苋尾状凝集素（ACL，ACA） BIOTIN-ACL
  Biotinylated Amaranthus Caudatus Lectin （ACL, ACA）
  Detection of Glycoproteins using Lectins in Histochemistry,
  ELISA, and Western Blot Applications

VECTOR 生物素苋尾状凝集素（ACL，ACA） BIOTIN-ACL

The following protocols offer guidelines for assay development using lectin-based detection of glycoproteins
present in tissue sections, adsorbed onto microtiter plates, or transferred from electrophoretic
gels onto nitrocellulose or PVDF membranes.
Histochemistry:
1a. Staining procedure for paraffin sections: Deparaffinize and hydrate tissue sections through
xylenes or other clearing agents and graded alcohol series and rinse for 5 minutes in tap water.
If required, retrieve antigens using the Antigen Unmasking Solution （H-3300 or H-3301）.
1b. Staining procedure for frozen sections: Air dry sections. Immediay before staining, fix
sections with acetone. Transfer slices to buffer. If endogenous enzyme activities are present,
inactivate using appropriate methods.
2. Perform Streptavidin/Biotin blocking if required following kit instructions （SP-2002）. Do not
use SP-2001. Block non-specific binding by incubating section with Carbo-Free™ Blocking
Solution （Cat. No. SP-5040） for 30 minutes at room temperature. Blot excess blocking solution
from the sections.
3. Apply biotinylated lectin at approximay 2-20 μg/ml in PBS （10 mM sodium phosphate, 150
mM NaCl, pH 7.4） to the sections and incubate for 30 minutes at room temperature. Wash with
TPBS （PBS + 0.05% Tween™20）.
4. Prepare VECTASTAIN®
® ABC （peroxidase, Cat. No. PK-6100） or VECTASTAIN® ABC-AP
（alkaline phosphatase, Cat. No. AK-5000） reagents according to the kit instructions. Apply to the
sections and incubate for 30 minutes at room temperature. Wash with TPBS.
5. Apply an appropriate precipitating substrate for the enzyme system used in step 4. For peroxidase,
ImmPACT™ DAB （Cat. No. SK-4105） is recommended; for alkaline phosphatase, Vector® Red
（Cat. No. SK-5100）. Rinse in tap water.
6. Counterstain （optional）, clear and mount. For galactose or GalNAc-specific lectins avoid mounting
in glycerol-based mounting media.
VECTOR 生物素苋尾状凝集素（ACL，ACA） BIOTIN-ACL

ELISA:
1. Adsorb target protein to microtiter plate by placing 50-200 μl of approximay 3 μg/ml glycoprotein
solution into the desired wells. Some wells may be left untreated as negative controls. Incubate at
37 ºC for 1 hour. Wash wells three times with TPBS （PBS + 0.05% Tween™20）.
2. Block non-specific binding by filling each well to the brim with Carbo-Free™ Blocking Solution
（Cat. No. SP-5040） for 30 minutes at room temperature. Wash wells three times with TPBS.
3. Apply 50-200 μl of approximay 2-20 μg/ml biotinylated lectin in PBS to the wells and incubate
for 30 minutes at room temperature. Wash wells three times with TPBS.

4. Prepare VECTASTAIN®
® ABC （peroxidase, Cat. No. PK-6100） or VECTASTAIN® ABC-AP
（alkaline phosphatase, Cat. No. AK-5000） reagents according to the kit instructions. Apply to the
wells and incubate for 30 minutes at room temperature. Wash wells three times with TPBS.
5. Apply an appropriate non-precipitating substrate for the enzyme system used in step 4. For
peroxidase, ABTS （Cat. No. SK-4500） is recommended; for alkaline phophatase, pNPP （Cat. No.
SK-5900）.
6. Quantify the colored reaction product by spectrophotometry.
Western Blot:
1. Perform electrophoresis and transfer proteins to a membrane according to standard procedures.
2. Block non-specific binding by incubating the membrane in Carbo-Free™ Blocking Solution （Cat.
No. SP-5040） for 30 minutes at room temperature. Use a sufficient volume to compley cover
the membrane.
3. Incubate membrane in PBS containing approximay 2-20 μg/ml biotinylated lectin for 30 minutes
at room temperature. Wash with TPBS （PBS +0.05% Tween™20）.
4. Prepare VECTASTAIN®
® ABC （peroxidase, Cat. No. PK-6100） or VECTASTAIN® ABC-AP
（alkaline phosphatase, Cat. No. AK-5000） reagents according to the kit instructions. Incubate the
membrane in the reagent for 30 minutes at room temperature. Wash with TPBS.
5. Apply an appropriate substrate for the enzyme system used in step 4. For peroxidase, DuoLuX™
Chemiluminescent/Fluorescent Substrate for Peroxidase （Cat. No. SK-6604） or ImmPACT™ DAB
（Cat. No. SK-4105） are recommended; for alkaline phosphatase, Chemiluminescent/Fluorescent
Substrate for Alkaline Phosphatase （Cat. No. SK-6605） or BCIP/NBT （Cat. No. SK-5400） are
recommended.
Negative Controls
Negative controls should be run in parallel in each of the above described methodologies to validate
binding results. When applying lectins, one of the most appropriate negative controls is to preabsorb
the lectin with a concentration of a defined sugar, with which, the lectin has a known high affinity.
Vector Labs offers a series of sugars that are intended for such a purpose.
The lectin is diluted to a suitable working concentration in a solution containing approximay
200 mM to 500 mM of the sugar. This mixture is left to bind at room temperature for 30 to 60 min.
Following this absorption incubation, the mixture is substituted into the procedure in place of the unabsorbed
lectin and incubated under the same conditions. The subsequent detection procedure is followed
as for the test method. In most cases the vast majority of lectin binding to the tissue section （membrane
blot, etc.） will be eliminated. Some trace binding to the section （blot etc） may still be present under
these conditions and probably indicates presence of secondary or tertiary sugar preferences. These negative
control results should be compared with the test results to determine specificity of binding

上海金畔生物科技有限公司代理VECTOR产品，咨询 详细产品信息

sciencell少突胶质细胞培养基OM

• 产品型号：  1621
• 简单描述
• sciencell少突胶质细胞培养基OM
  货号1621
  产地美国
  缩写OM
  规格500 ml
  用途科研
  包装瓶装
  保存4℃冰箱
  库存状态：现货

sciencell少突胶质细胞培养基OM，sciencell少突胶质细胞培养基OM ， 

少突胶质细胞培养基包含500 ml基础培养基，2.5mll胎牛血清（FBS, Cat. No. 0002） 、5ml    少突胶质细胞生长因子（OGS, Cat. No. 1662）、5 ml青霉素/链霉素溶液(P/S, Cat. No. 0503) 。

少突胶质细胞培养基是专门为正常人类未成熟和成熟的少突胶质细胞体外培养设计的zui适于其生长的培养基。是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(0.5%)。该培养基缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类微血管内皮细胞体外培养中的增殖和生长，并为其达到营养平衡状态。

GE 葡聚糖凝胶SEPHADEX LH-20

• 产品型号：  17-0090-01
• 简单描述
• GE 葡聚糖凝胶SEPHADEX LH-20
  25G、100G、500G装价格优惠
  Sephadex™ LH-20
  优秀的脂类，激素，维生素，和其他生物小分子分馏。专为在极性有机溶剂和水溶剂混合物中使用。

GE 葡聚糖凝胶SEPHADEX LH-20
 

Sephadex™ LH-20

• Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules. Designed for use in polar organic solvents and aqueous solvent mixtures.
• Since Sephadex™ LH-20 has exceptional properties, we recommend that you consult the publications listed under Related Products.

葡聚糖™LH – 20技术资料：
 

Sephadex™ LH-20

Technical Information

Millipore 0.8um混合纤维素酯微孔滤膜厂家

• 产品型号：  AAWP04700
• 简单描述
• Millipore 0.8um混合纤维素酯微孔滤膜厂家：致力于在各个领域内向用户提供水平和质量稳定的产品，同时也为客户提供相关的实验服务。 公司主要代理 Abcam、R&D、Sigma、NALGENE、WHATMAN、Millipore等。

Millipore 0.8um混合纤维素酯微孔滤膜厂家说明:

商标名: MF-Millipore

数量/包装: 100

滤膜材质: Mixed Cellulose Esters

滤膜商标名: MF-Millipore

折射率: 1.51

水通量，mL/min x cm2: 190

23 °C 时的泡点: ≥1.10 bar

zui高操作温度，°C: 75

滤膜类型: 表面滤膜

滤膜孔径，?m: 0.8

可润湿性: 亲水

滤膜直径，mm: 47

滤膜代码: AAWP

滤膜颜色: 白色

Millipore 0.8um混合纤维素酯微孔滤膜厂家技术参数：

产品名称: MF-Millipore 表面滤膜

滤膜表面: 光面

重量分析溶出物，%: 4

厚度，?m: 180

空气流速，L/min x cm2: 16

孔隙率 %: 82

详细：

公司介绍：

上海金畔生物科技有限公司，是一家专注于生命科学和技术领域的企业，主要从事分子学、细胞学、免疫学、蛋白组学等实验研究的相关、耗材及仪器的销售，致力于在各个领域内向用户提供世界水平和质量稳定的产品，同时也为客户提供相关的实验服务。 公司主要代理 Abcam、R&D、Sigma、NALGENE、WHATMAN、Millipore等，和供应商有着良好的合作关系，同时致力于不断完善自身的供应体系，以期能更好的服务于国内的科研用户。