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Products > Optimize Reagents > Optimize – Buffers > HEPES sodium Buffer
HEPES sodium Buffer
Applications
- Crystallization grade HEPES sodium buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
HEPES sodium
Synonyms: HEPES sodium salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, or N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
C8H17N2NaO4S
Mr 260.29
CAS [75277-39-3]
EC Number 278-169-7
MDL Number MFCD00036463
PubChem Substance ID 24895780
pKa 7.5 at 25°C
Useful pH Range of HEPES sodium: 6.6 – 8.2
HR2-733 is titrated to pH 7.5 using HCl
HR2-733 measured Conductivity range: 41.7 – 43.4 mS/cm at 25°C
HR2-733 measured Refractive Index range: 1.37577 – 1.37766 at 20°C
HR2-577 is the free base of HEPES sodium and is to be titrated to the desired pH using HCl
HR2-577 measured pH Range: 10.2 – 12.1 at 25°C
HR2-577 measured Conductivity range: 25.9 – 27.2 mS/cm at 25°C
HR2-577 measured Refractive Index range: 1.37097 – 1.37298 at 20°C
HR2-931-01 – 1.0 M HEPES sodium pH 6.8 is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.
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CAT NO
HR2-577
NAME
DESCRIPTION
100 mL
PRICE
$122.00
cart quote
CAT NO
HR2-733
NAME
DESCRIPTION
100 mL
PRICE
$122.00
cart quote
Support Material(s)
HR2-577 HEPES sodium SDS
HR2-733 HEPES sodium pH 7.5 SDS
Titration TableCertificate Of Analysis
Related Item(S)
- Hydrochloric acid
- StockOptions Sodium HEPES Buffer Kit
- Individual StockOptions Sodium HEPES Reagents
References
1. Crystallization and preliminary X-ray characterization of GM2-activator protein. C. S. Wright and S.-C. Li. Acta Cryst. (1997). D53, 211-212.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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