NDSB-256蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

NDSB-256
NDSB-256

NDSB-256

NDSB-256

NDSB-256

NDSB-256

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-256

NDSB-256

Applications

  • Crystallization grade NDSB-256 for formulating screens or for optimization

Features

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability

Description

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-256
Synonyms: Dimethylbenzylammonium propane sulfonate
C12H19NO3S
Mr 257.37
Purity: ≥99% by TLC
Hygroscopic
Store at room temperature (+20 degrees Celsius)

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NDSB-256

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NDSB-256

NDSB-256

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NDSB-256

CAT NO

HR2-705

NAME

NDSB-256

DESCRIPTION

5 grams

PRICE

$52.00

cart quote

Support Material(s)

Certificate Of Analysis

References

1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).

2. A nondetergent sulfobetaine prevents protein aggregation in microcalorimetric studies. Collins et al, Notes & Tips / Anal. Biochem. 352 (2006) 299-301.

NDSB-256 NDSB-256

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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