Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A (see Figure 1 in Supporting Data), B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.
Highlights
Performs under a wide range of reaction conditions
Protects RNA from degradation at temperatures up to 55°C (see Figure 2 in Supporting Data)
Note
DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity.
Specifications
CategoryName
Concentration
40 U/µL
Definition of Activity Unit
One unit of the RiboLock RNase Inhibitor inhibits the activity of 5 ng RNase A by 50%.
Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/mL BSA, 100 ng/mL RNase A, 0.1 mg/mL [3H]-RNA, 50 mg/mL yeast RNA, 8 mM DTT.
Hazardous
No
Hazardous:
No
Inactivation
Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min heating at 70°C.
Inhibition
Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound.
Molecular Weight
49.6 kDa monomer
Quality Control
The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases confirmed by appropriate quality tests.
Functionally tested in RNA and cDNA synthesis.
Shelf Life:
Shipping Condition:
Shipping Information
Source
E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor.
SpecificationName
SpecificationValue
Storage Buffer
The enzyme is supplied in 20 mM HEPES-NaOH (pH 7.5), 50 mM NaCl, 8 mM DTT, 0.03% v/v) ELUGENT Detergent and 50% (v/v) glycerol.
Storage Condition
-20 C
Storage Condition:
fermentasRNA酶抑制剂RiboLock RNase Inhibitor 火热促销中
促销产品明细：
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500
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目录价￥
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