qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒

qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒

  • 产品型号:  59824
  • 简单描述
  • qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒
    对来自基因组DNA、福尔马林固定石蜡包埋(FFPE)样本、细胞或组织样本的DNA进行超快速、高效的亚硫酸氢盐转化
详细介绍

qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒

  • 优化的操作流程对胞嘧啶转化率超过99%
  • 新型Bisulfite Solution可在少至30分钟内完成有效地亚硫酸氢盐转化
  • 可用于基因组DNA、全血、培养细胞、组织样本和福尔马林固定石蜡包埋(FFPE)切片
  • 快速、简洁的操作流程,有效转化和纯化DNA
  • 独特的DNA Protect技术可防止DNA降解

EpiTect Fast Bisulfite Conversion Kits可在少至30分钟内将未甲基化的胞嘧啶高效转化成尿嘧啶,起始材料选择也有出色的灵活性。简化的样本裂解操作流程和直接的DNA亚硫酸氢盐转化使转化过程快速、省力,样本包括全血、培养细胞、组织样本、基因组DNA和福尔马林固定石蜡包埋(FFPE)切片。创新的DNA Protect技术确保有效的DNA变性而不会降解,得到高产量DNA和可重复性结果。此外,转化DNA可在少至10 µl洗脱缓冲液中洗脱,得到高浓度DNA。

qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒

 

Performance

高效亚硫酸氢盐转化 

在甲基化分析中,对DNA亚硫酸氢盐处理,将未甲基化的胞嘧啶转化成胞嘧啶是zui关键的步骤,因为反应效率会对下游分析的可靠性造成很大影响。EpiTect Fast Bisulfite Conversion Kits含有的创新型Bisulfite Solution可进行超快速、高效的亚硫酸氢盐转化,甚至可处理难分析的样本,如来自女性样本的X染色体基因组DNA,因为2个X染色体中有一个是高度甲基化的(参见”Highly efficient cytosine conversion of X chromosome DNA”)。焦磷酸测序对胞嘧啶转化定量分析表明,与其他供应商的试剂盒相比,EpiTect Fast Bisulfite Conversion Kit的转化效果明显更好,特别是当起始材料的量很有限时(参见”Superior bisulfite conversion”)。

 

订购信息:

 

Cat No./ID: 59802

EpiTect Fast Bisulfite Kit (10)

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Trial kit for 10 preps: Deparaffinization Solution, Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers

Cat No./ID: 59824

EpiTect Fast DNA Bisulfite Kit (50)

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For 50 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers

Cat No./ID: 59826

EpiTect Fast DNA Bisulfite Kit (200)

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For 200 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers

Cat No./ID: 59864

EpiTect Fast LyseAll Bisulfite Kit (50)

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For 50 preps: Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers

Cat No./ID: 59866

EpiTect Fast LyseAll Bisulfite Kit (200)

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For 200 preps: Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers