qiagen质粒大量提取试剂盒12162 12163

qiagen质粒大量提取试剂盒12162 12163

  • 产品型号:  
  • 简单描述
  • qiagen质粒大量提取试剂盒12162 12163
    纯化至多10 mg转染级质粒或柯斯质粒DNA
    纯化得到相当于连续2次CsCl梯度离心得到的DNA纯度
    高产量质粒DNA
    经济的制备方法
    使用LyseBlue,获得的裂解效果和Z大的DNA产量
详细介绍

qiagen质粒大量提取试剂盒12162 12163

QIAGEN Plasmid Kit procedures.

Neutralized bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer. The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.

qiagen质粒大量提取试剂盒12162 12163

Performance

QIAGEN Plasmid Kit利用重力流QIAGEN阴离子交换柱技术高效纯化质粒DNA。产量可达10 mg(Giga)、2.5 mg(Mega)、500 µg(Maxi)、或100 µg(Midi)高拷贝质粒DNA(培养体积取决于质粒拷贝数、插入片段大小、宿主菌株和培养基。)QIAGEN Plasmid Kits纯化的质粒DNA适合多种应用

订购信息:

12162质粒大量提取试剂盒QIAGEN Plasmid Maxi Kit (10) 规格:10次

12163质粒大量提取试剂盒QIAGEN Plasmid Maxi Kit (25) 规格:25次