VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)

VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)

  • 产品型号:  PK-6100
  • 简单描述
  • VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)
    规格:1KIT
    试剂盒组份:

    2ml试剂A(Avidin DH溶液)
    2ml试剂B(生物素化的酶)
    1ml生物素化的二抗(1.5mg anti-IgG/0.5mg anti-IgM抗体/2.1mg通用抗体)
    3ml阻断血清
    其中standard试剂盒只含有试剂A和试剂B
详细介绍

VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)

Vector Laboratories公司是世界著名的生物学检测试剂公司。1977年首先开发出了生物素-亲和素系统,该产品系列是检测方法上的重要革命。之后经过不懈的努力,VectorLaboratories公司发展出ABC技术(Avidin : Biotinylated Enzyme ComplexTechnology,亲和素-生物素-过氧化物酶复合物技术)并建立了著名的VECTASTAIN@ ABC系统,该系统被普遍视为目前zui灵敏、zui可靠与zui有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。而该系统仍在不断地发展,以满足广大研究人员的各种不同要求(如多抗原的标记检测)

VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)

ABC技术利用生物素和卵白素具有极高亲和性的生物学特征,将卵白素和生物素化辣根酶按照一定的比例混合,形成ABC复合物。该法亦被称作三步法,*步为biotin化二抗和一抗结合,第二步为avidin(亲和素,A液)和二抗上的biotin结合,第三步为HRP偶联的biotin(B液)和avidin结合,而HRP再催化底物显色完成染色。

VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)

 

Features:

  • Advanced avidin/biotin technology:  The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
  • HIghest sensitivity, low background:  The VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system.  The Elite® ABC series is approximay 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.
  • Cost effective:  HIgher sensitivity leads to lower cost per slide
  • Available without (“Standard” kit) or with biotinylated species-specific or universal secondary antibodies
  • Available in ready-to-use formats:  Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Kit reagents.